spelled it out for ya. doesn't get much easier than that.

agent orange

How are you supposed to get the chunk of the master into the new sterilized jar without opening it, & diggin into the substrate to put the chunk in?.....and without contaminating it with one your hands, and two air borne contaminations when you open it and starting diggin around in there>

Jar 1 = 100% colonized Jar
Jar 2 = Freshly Sterilized Substrate (less than 24 hrs old)

To do the transfer lock yourself in a room, shut off all fans, close all windows and seal anything that could cause a draft. Now drench the room in Lysol, let it sit for 5 mins, then repeat. When this layer has settled start the transfer. It's also useful to use a particle mask (or something to protect you from breathing all over your work-area (use mouthwash before doing this also, cuts the possibility of contamination even more).

Open Jar 1 and Jar 2 using SLOW methodical movements and pour 10% of Jar 1 into Jar 2. Close the lids on both jars and shake Jar 2 to distribute the innoculant. This is why I like Rye Grain, it's easy to shake and it's a fast and nutrient filled (besides, Paul Stamets likes it too :P).

I usually don't worry about contaminants beyond the steps mentioned. The reason is since the mycelium is already healthy and virile, it will usually overtake and and all contaminants in Jar 2 before they get a foothold. Just be smart when doing the transfers.

Repeat for Jars 3-10 (using 10% in each jar).

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"IT's two in the morning.....Do you know where your flashlite and paper bag are?"