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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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Offlinedr4g0n
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Registered: 12/08/02
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Mixing partially colonized substrate up?
    #1301627 - 02/12/03 06:27 AM (13 years, 9 months ago)

I have a small jar i was testing a cloning experiment on, and the experiment was a sucess. Since it was small I decided to try something else. I know i run the risk of contams when opening the jar, but I decided to do it just to try an experiment. I was wondering, if you mix the mycelium around in the substrate, so that there is mycelium broken up around different parts of the jar, will it colonize the jar quicker? I think it will, as the rhyzophormic (sp?) grow grows outwards from the epicenter. If it works and is done right, and you are a risk taker, theoretically you can have a fully colonized jar in less then a week? Ill post my results, assuming this already hasnt been done


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OfflineAnnoA
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Re: Mixing partially colonized substrate up? [Re: dr4g0n]
    #1301642 - 02/12/03 06:35 AM (13 years, 9 months ago)

what kind of substrate?
You can and should shake grains, but it?s nearly impossible to shake brf+vermiculite.


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Offlinedr4g0n
sleeps withdragons

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Re: Mixing partially colonized substrate up? [Re: Anno]
    #1301748 - 02/12/03 07:51 AM (13 years, 9 months ago)

Not shake, but stir. Im using BRF with verm and a little bit of bird seed. Im saying dusrupt the mycelial network. I did it with a screwdriver, flame sterilized. I mixed it around, pushing some mycelium to the bottom of the jar, and leaving some at the top. Im wondering if the mycelium network will also start at the bottom, and reconnect in the middle. Making it colonize faster...


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Edited by dr4g0n (02/12/03 08:22 AM)


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OfflineAnnoA
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Re: Mixing partially colonized substrate up? [Re: dr4g0n]
    #1302124 - 02/12/03 10:43 AM (13 years, 9 months ago)

The chances of contaminating the whole thing are pretty high, IMO.


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Offlinedr4g0n
sleeps withdragons

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Re: Mixing partially colonized substrate up? [Re: Anno]
    #1302140 - 02/12/03 10:50 AM (13 years, 9 months ago)

you really think so? even when things like casings go uncontaminated?


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Re: Mixing partially colonized substrate up? [Re: dr4g0n]
    #1302153 - 02/12/03 10:57 AM (13 years, 9 months ago)

But casings are different. You case completely colonized substrate.

But what you are doing is handling uncolonized substrate. And this is susceptible to contaminates.


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Offlinesirreal
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Re: Mixing partially colonized substrate up? [Re: dr4g0n]
    #1302170 - 02/12/03 11:03 AM (13 years, 9 months ago)

Casings are more contam resistant because the nutrients have been mostly consumed. But, I did what you are talking about when I used a partially colonized BR jar( half pint) and transferred some myc. to birdseed. I mixed up the uncolonized part really good(about half of a jar) and incubated it for another three days then made a small casing with it. I have 16 mushrooms on that casing right now. I also have 6 fully colonised pint jars of birdseed.

I want to add that I soaked the half pint of myc. in a peroxide mixture before I made the casing. That might have helped some.


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Edited by sirreal (02/12/03 11:11 AM)


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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