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OfflineLCid
Close ToInsanity.
Registered: 09/12/01
Posts: 1,248
Loc: Seattle, WA
Last seen: 19 years, 9 months
taping holes...
    #698046 - 06/24/02 09:38 AM (22 years, 5 months ago)

i knwo thi squestion has been answered a thousand times ive spent the last hour going throught the threads but have failed to find a real answer or maybe i have

if you tape holes

pros.
Less Risk Of Contamination
Cons
Slower


what im wodnering is how much slower?

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Offlineskatepunk2k2
newbie
Registered: 04/26/02
Posts: 48
Last seen: 20 years, 5 months
Re: taping holes... [Re: LCid]
    #698101 - 06/24/02 10:09 AM (22 years, 5 months ago)

i tried one jar taped and one with foil over it...the foil jar colonized much quicker...once i pulled the tape off and covered it with foil it colonized much quicker.

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OfflineLCid
Close ToInsanity.
Registered: 09/12/01
Posts: 1,248
Loc: Seattle, WA
Last seen: 19 years, 9 months
Re: taping holes... [Re: skatepunk2k2]
    #698146 - 06/24/02 10:40 AM (22 years, 5 months ago)

y is this is air exchange relaly importaint for mycelum?

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OfflineMycelium
THC Junkie
Male
Registered: 06/14/02
Posts: 463
Last seen: 5 years, 8 months
Re: taping holes... [Re: LCid]
    #698190 - 06/24/02 10:56 AM (22 years, 5 months ago)

Because living things need air(oxygen) to live/grow

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InvisibleMushroom_Madness
enthusiast
Registered: 01/10/02
Posts: 261
Loc: Quaint New England Villag...
Re: taping holes... [Re: LCid]
    #698192 - 06/24/02 10:57 AM (22 years, 5 months ago)

yes, air exchange will contribute to faster colonization, as well as the reight temperature and moisture level my friend.

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Offlinebagmandsl
member
Registered: 03/19/02
Posts: 112
Last seen: 18 years, 10 months
Re: taping holes... [Re: LCid]
    #698288 - 06/24/02 11:57 AM (22 years, 5 months ago)

Taping is not needed just cover with foil

www.thesporehaven.com
buy 4 get 2 free all syringes $6.00

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InvisibleZestyMycelium
journeyman
 User Gallery
Registered: 06/22/02
Posts: 119
Re: taping holes... [Re: LCid]
    #698317 - 06/24/02 12:16 PM (22 years, 5 months ago)

The first time I tried I didnt tape the holes, and lost most of my jars to contamination. After I started taping holes, I have nearly a 100% survival rate, and Im still using a big stock pot for sterilization.
I cant contribute all of the success to the tape, because I know my sterilization methods have improved. One thing I would worry about is anerobic bacteria if you tape - I remove mine when the jar is about 90% colonized.
Also - if you use a heating pad to heat your incubator, your jars may dry out if they're not taped.

Just some pro's for taping...::)

-Man, that Mycelium is ZESTY!!-

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OfflineShroomNewb
enthusiast
Registered: 11/08/01
Posts: 230
Last seen: 18 years, 10 months
Re: taping holes... [Re: ZestyMycelium]
    #698911 - 06/24/02 06:25 PM (22 years, 5 months ago)

I failed like 3 times because of not taping my holes.. unless you use a elastic band or something with your foil water will get into your jars wetting your dry vermiculite barrier.. which will then attract contams.

When I innoculate I don't bother to retape the holes.

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Offlinestaindblue
enthusiast

Registered: 04/14/02
Posts: 384
Loc: GodsCountry
Last seen: 18 years, 4 months
Re: taping holes... [Re: LCid]
    #699006 - 06/24/02 07:16 PM (22 years, 5 months ago)

I never tape the holes, My jars colonize fast and i have a low contamiation rate.
The contamination I've experienced in the past was do to other factors( Sterilizing)
or the lack of sterilizing properly. Not because the holes wheren't taped i'm sure of it.

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OfflineCrabbyAss
sharp shooter
Registered: 06/02/02
Posts: 176
Last seen: 3 years, 5 months
sex [Re: Mycelium]
    #699493 - 06/25/02 12:04 AM (22 years, 5 months ago)

rancid

Edited by CrabbyAss (08/23/02 12:07 PM)

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OfflineEightball
whore consumer
Male User Gallery

Folding@home Statistics
Registered: 07/21/01
Posts: 3,013
Last seen: 10 years, 4 months
Re: taping holes... [Re: LCid]
    #699500 - 06/25/02 12:23 AM (22 years, 5 months ago)

TAPE YOUR HOLES!

i know this from experience and its not worth losing a batch of jars b/c you thought you could be superman and keep microscopic mold spores from floating in the jars. air exchange is NOT important to mycelium because high CO2 enviroments promote the vegatative stage of growth. when you birth them, you will give them a shock with the high O2 content of the air and fruiting will begin. yes its true that on average a jar with a polyfill filter or filterdisc will colonize faster, but this is mainly with pints and quarts of GRAIN substrate where the amount of air trapped inside the jar will not contain enough O2 to allow 100% colonization to take place . there is no reason for any kind of air exchange to be taking place with standard PF cakes because they have enough trapped air to supply all the mycelium that will grow in that little 1/2 pint volume. if you want faster colonization, just keep the temps in the low 80s and you will have uncontaminated colonized cakes in no time. good luck.


--------------------
If you're frightened of dying and you're holding on.you'll see devils tearing your life away.
But...if you've made your peace, then the devils are really angels
Freeing you from the earth.

Edited by Eightball (06/25/02 12:27 AM)

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InvisibleShaw

Registered: 06/27/01
Posts: 2,263
Re: taping holes... [Re: LCid]
    #699607 - 06/25/02 03:17 AM (22 years, 5 months ago)

ize used ALUMINUM INSULATION TAPE from the beginning. PC's excelent. complete percect seal. it's all i happened to have at the time and works perfect. Looks medical grade. i fold 1 edge for easier removal.


--------------------

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