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Offlinejoeinc
journeyman
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Registered: 07/01/02
Posts: 113
Loc: Brooklyn, NYC
Last seen: 11 years, 30 days
innoculation holes
    #722573 - 07/04/02 02:22 PM (15 years, 3 months ago)

I innoculated 24 jars with 4 different strains (B+, EQ, GT and the Free Fla Strain from littleguy) on monday. This is my first atempt at this so I might be a little over cautious.
My question is: Should the innoculation holes be sealed immediately after the jar is done, or should the holes be left open for some CO2 out??
right now I have them sealed and about 30% of the jars are already showing white spots on the glass. I just wanna make sure I dont cause a problem by having
the holes sealed. again.. I might just be overly paranoid about contamination. Shrooms are hard to come by here in NYC


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Invisiblephrozendata
Carpal Tunnel

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Registered: 04/24/00
Posts: 5,015
Re: innoculation holes [Re: joeinc]
    #722598 - 07/04/02 02:42 PM (15 years, 3 months ago)

They are allready colonizing so just leave them the way they are.


--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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OfflineMacey Howard
Formally MOE HOWARD
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Registered: 07/03/99
Posts: 14,165
Loc: Georgia
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Post deleted by Moe Howard [Re: joeinc]
    #722599 - 07/04/02 02:42 PM (15 years, 3 months ago)



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Offlinejoeinc
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Registered: 07/01/02
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Loc: Brooklyn, NYC
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Re: innoculation holes [Re: Macey Howard]
    #722881 - 07/04/02 06:40 PM (15 years, 3 months ago)

thanks, took your advice, took the tape off some of the ones that haven't started yet.. I guess its a learning experience


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Offlinedracon
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Registered: 08/29/01
Posts: 96
Last seen: 12 years, 3 months
Re: innoculation holes [Re: joeinc]
    #723350 - 07/04/02 09:28 PM (15 years, 3 months ago)

I loosely cover the top of the jar with tinfoil, just to ward off the stray breeze carrying bad guys. Allthough there is no need to tape it doesn't hurt, but you may have to take it off and flip if they stall


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OfflineGreyMatter
enthusiast

Registered: 03/26/02
Posts: 329
Loc: USA
Last seen: 13 years, 3 months
Re: innoculation holes [Re: dracon]
    #724106 - 07/05/02 06:08 AM (15 years, 3 months ago)

The jars I taped (of the same batch as the ones not taped) colonized slower than the others. I have never needed to use tape.


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OfflineChromeCrow
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Re: innoculation holes [Re: joeinc]
    #724183 - 07/05/02 07:22 AM (15 years, 3 months ago)

i never tape anymore. soon as i nock em up, i flip em. colonization time has been cut in half, at least, over the time it took when the holes are taped


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Offlinetchyted
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Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 3 years, 22 days
Re: innoculation holes [Re: joeinc]
    #725412 - 07/05/02 09:06 PM (15 years, 3 months ago)

okay, at the risk of taking you into the deep end too far, i'm going to reccomend that you find some plastic mason jar lids, usually found where good selection of mason jars are sold. we no longer play with the holes in the lid at all, but this may be more than you are prepared for. but using the plastic lid will make it so that if it is determined that you need to increase gas exchange, it is as simple as loosening the lid a turn or so.

in principle, you shouldn't need gas exchange at all, but usually your jar will not be perfectly sterile. the mushroom mycelium can survive in an anerobic environment. BUT they don't tolerate CO2 very well. the mycelium itself produce CO2 and so do common benign contams like yeast that often come along with your spores.

there is no universal answer to the tape or no tape question, since it must be determined if the mycelium is being smothered by CO2. in some cases more gas exchange will speed growth. in others, it will only introduce more contams.

as a general rule of thumb, if your mycelium seems to be stalled or slow growing, you need to increase gas exchange. many people use special filter discs instead of the lid with holes, and the increased gas exchange has been known to shorten collonization time by leaps and bounds, also this tends to eliminate contams on several fronts. it is one more thing to buy, however, and it is something that has to be ordered, so this is why i'm telling you to get the plastic lids. you don't need them, but it does make the process smoother.



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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: innoculation holes [Re: tchyted]
    #725485 - 07/05/02 09:36 PM (15 years, 3 months ago)

A Raccoon I know uses plastic lids w/2 holes of about 1/4 inch. One in the center of the lid & another to the side. He uses a filter disk inside the jar & innoc's through the center hole & just swivels the syringe -- to squirt one in the center & in each corner of the jar & tapes it closed whan done.

The object with one puncture is less of a chance for any contam getting in verses 4 punctures & needle removal. Once it's taped, he places a double or triple coffee filter on over the lid & double wraps a thick rubber band around it. To keep any nasties drifting around from settling on the filter disk hole.

If done in a glove box or in front of a flow hood -- he may lose 1 or 2 jars out of 100 to 200. If done at the kitchen drain board he may lose 1 or 2 out of 24.

Just figure out what works for you & go with it. Simple, clean & quick is better.
SixTango


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


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OfflineAnnoA
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Re: innoculation holes [Re: tchyted]
    #725650 - 07/06/02 12:35 AM (15 years, 3 months ago)

>the mushroom mycelium can survive in an anerobic
>environment. BUT they don't tolerate CO2 very well.

At this stage high levels of CO2 are actualy contributing to faster colonisation.
Read Stamets? TMC for more information.


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Offlineindkebr
member
Registered: 06/21/02
Posts: 169
Loc: Colorado
Last seen: 6 years, 6 months
Re: innoculation holes [Re: Anno]
    #726297 - 07/06/02 12:12 PM (15 years, 3 months ago)

NO such thing as over cautious with this hobby.


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OfflineOrbita6974
journeyman
Registered: 07/05/02
Posts: 71
Last seen: 15 years, 2 months
Re: innoculation holes [Re: indkebr]
    #726461 - 07/06/02 02:11 PM (15 years, 3 months ago)

can you take the filter disk and use it in place of the lid and clamp it down with the ring?? from what i read i think that would take alot of the guess work out of tapeing..or not tapeing because your air flow would be maximized and no contaminants can get in. or am i just a noob


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