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BRF "Agar substitute" tek
    #6854799 -

Hey,

I was just reading the BRF Agar Substitute tek, which received five stars. I assume that since it got five stars, it works nicely.

Just wondering: Any words of wisdom or caution if using the BRF agar substitute to try to isolate, even if not a pure strain, a collection of generally fast-colonizing well-fruiting strains?

Also, is there any reason one could not inoculate the BRF-"Agar" with with mycelium from a Karo-based LC?

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Re: BRF "Agar substitute" tek [Re: lsg1]
    #6855021 -

Worthless for strain isolation. It's just a small pf jar, not agar or an agar substitute. We got rid of the thread rating system. Anyone could give a thread ratings, and the system got abused to the point that the ratings were useless.

If you want to isolate strains, or isolate away from contamination, you need the flat plane of a petri dish, and lots of them. It takes several transfers to get away from contamination or towards single sector isolated strains, and using jars would be messy and take up a LOT of room. You also want the mycelium growing on top of the medium, not all the way through it as you get with a thin layer of brf.
RR


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Re: BRF "Agar substitute" tek [Re: RogerRabbit]
    #28744929 -

Wouldn't this tek be really good for truffles? In a sense you'll get some little brown rice flour Jars with 2 Injection Ports. In one and out the other😭. Like a contamination filter, but I was thinking to use this tek where you put agar in jars wit the 2 injection ports instead of opening to contamination air just use needles in the jars.

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