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Agar substitute

Illustrated agar medium substitute technique prepared with brown rice flour.

Those of you who would like to study mycelium and can't find agar, can't afford it, or whatever your reason may be, can use this little technique.

This tek is very newbie friendly, cheap to prepare, and some seasoned cultivators may find it useful as well.
Try it, you may enjoy it.

All of the ingredients combined = Total cost, $1.50 (current price of Rice). The Tyvek can be obtained at your local Post Office for freePriority Mail Envelopes .

  • Brown Rice
  • water
  • 1/2 pint jars
  • Tyvek
  • foil
  • coffee grinder

Preparation of media(for each jar):

  • 1->5 grams of Brown Rice ground into a fine powder.
  • 2->16 ml (cc) of water.
  • 3->place these ingredients in each jar you plan to use.
  • 4->place Tyvek filter on jar, apply disc/polyfill filter and ring, cover with foil.
  • 5->PC them for 15 minutes at 15 psi and allow to cool.

For a thinner layer:
  • 2.5 grams of Brown Rice ground into a fine powder
  • 8 ml (cc) of water

To inoculate with a spore syringe (multispore) simply flame needle, wipe with alcohol,  and plunge syringe in through the polyfill,  tyvek and inject 1 (one) drop of spore solution in the center of media.
Remove syringe, replace foil and incubate at 80°F - 85°F.

This colonizes 100% in about 2 weeks or less (less using the thin layer) .

Once you have the growth you are looking for you can transfer a wedge to the grain of your choice.

This is has worked well for me, and I hope you can find it useful.

by doc34

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