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OfflineLaCasta
Beyond Good andEvil

Registered: 05/23/02
Posts: 1,332
Loc: Chicago
Last seen: 7 years, 8 months
Attempting to re-innoculate
    #659141 - 06/02/02 03:10 PM (16 years, 1 month ago)

A friend of mine innoculated 1/2 pint jars and a grow bag. Noticing no growth in either its obvious the syring is to blame. I was wondering if it is possible to innoculate these same jars and grow bag with another syringe?

Thanks


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"Memories of high speeds when the cops crash, As I laugh pushin the gas while my glocks blast" -RIP Tupac


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Offlinebassplayer74
old hand

Registered: 11/24/01
Posts: 906
Loc: Tennessee
Last seen: 7 years, 9 months
Re: Attempting to re-innoculate [Re: LaCasta]
    #659187 - 06/02/02 03:47 PM (16 years, 1 month ago)

It's risky.

How long has it been since innoculation?

Keep in mind, its possible for it to take 3 weeks till you see growth.

And, its not "obvious" that the syringe is to blame. Was your substrate sterilized properly? Did you wait for it to COMPLETELY cool before you innoculated?


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If you will it, it is no dream.


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OfflineLaCasta
Beyond Good andEvil

Registered: 05/23/02
Posts: 1,332
Loc: Chicago
Last seen: 7 years, 8 months
Re: Attempting to re-innoculate [Re: bassplayer74]
    #659218 - 06/02/02 04:14 PM (16 years, 1 month ago)

The jars and bag were innoculated five days ago with a solution called theKeeper's Brew With this you are suppose to see mycelium growth within 24-72 hours and supposedly reduce the colonization time. So i assumed that it was bunk considering how long its taken and both the bags and jars show no sign of growth.


--------------------

"Memories of high speeds when the cops crash, As I laugh pushin the gas while my glocks blast" -RIP Tupac


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OfflineStainBlue
Da Man
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Registered: 04/11/02
Posts: 392
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Re: Attempting to re-innoculate [Re: LaCasta]
    #659259 - 06/02/02 04:57 PM (16 years, 1 month ago)

Did you see mycelial growth in your keeper's brew? I have never used the brew but from what I'm understanding, the brew promotes mycelial growth inside the jar. Then you innoculate your jars with the mycelial growth. I think that's how it is supposed to work, but I'm not sure. So if there was no growth in your brew you might not get growth in your jars or bag. Someone correct me if I'm wrong.


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Peace,
StainBlue


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OfflineDrummer
CelestialMechanic
Male

Registered: 08/16/00
Posts: 703
Loc: SE Michigan
Last seen: 6 days, 23 hours
Re: Attempting to re-innoculate [Re: LaCasta]
    #659359 - 06/02/02 06:30 PM (16 years, 1 month ago)

I had a similar problem with 3 bags, the syringe used was not viable. After a few weeks with no signs of growth, mycelial or contams, I re-innoculated with a viable syringe. I had very good luck the second time. I used a minimal amout of solution the second time because I did not want to make the bags too wet inside.


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People don't want other people to get high, because if you get high, you might see the falsity of the fabric of the society we live in.

-Ken Kesey


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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