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Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag

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OfflineTrippingBillies
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Registered: 04/09/02
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Sterilization through Boiling/Steaming
    #639985 - 05/22/02 05:36 AM (22 years, 13 days ago)

Hey guys, I've yet to attain a pressure cooker and have some questions regarding sterilization through boiling. In the past I've had only about a 50% success rate with my method and I would like some input. My standar operating procedure is to boil the jars for 1 hour with a low water level and sometimes I use a vegitable steamer. Last week I mad 12 jars and innoculated batches of 6, with a different syringe for each 6. I lost 6 jars to contams but can trace that back to a poorly made syringe. For my own interest please leave a detailed description of your own personal boiling/steaming methods. For all those out there, I have RTFM many times, and many versions. Just curious about everyone else's procedures for sterilization. Thanks.


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OfflineSeussA
Error: divide byzero

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Re: Sterilization through Boiling/Steaming [Re: TrippingBillies]
    #640006 - 05/22/02 05:51 AM (22 years, 13 days ago)

I don't use this method myself because I have a PC, but...

Boil the jars just like you do now. Let them sit for 12-24 hrs, and then do it again. If you still have problems, add another 12-24 hrs rest period and a third boil. This method has some scientific name which I can't remember right now.


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OfflineGaNjAShRooM
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Registered: 02/22/02
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Re: Sterilization through Boiling/Steaming [Re: Seuss]
    #640032 - 05/22/02 06:07 AM (22 years, 13 days ago)

This method has some scientific name-its called boiling your jars-lol


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OfflineAnnoA
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Re: Sterilization through Boiling/Steaming [Re: GaNjAShRooM]
    #640046 - 05/22/02 06:13 AM (22 years, 13 days ago)

This method is called tyndallization or fractional sterilisation.

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OfflineTrippingBillies
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Re: Sterilization through Boiling/Steaming [Re: Anno]
    #640054 - 05/22/02 06:18 AM (22 years, 13 days ago)

Anno, what do you do for a living, you are an informational superhighway all by yourself!


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OfflineSeussA
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Re: Sterilization through Boiling/Steaming [Re: Anno]
    #640093 - 05/22/02 06:40 AM (22 years, 13 days ago)

Thats it Anno! So how do you fit that encyclopedia in your head, and where can I pick one up?


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