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Amazon Shop: ½ Pint Jars, 10cc Syringe, Vermiculite

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Offlineahaig
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Registered: 10/15/01
Posts: 13
Last seen: 15 years, 6 months
Honey Water Tek Innoculation - Slow/No Growth?
    #608126 - 04/14/02 04:13 PM (16 years, 1 month ago)

I've started two strains through the honey water tek: mex. cubs and T3 Thai. The jars have been growing well (a substantial dense mycelium growth floats on one at the top and the other at the bottom of the jar). A few days ago I used them to innoculate 6 containers filled with alien substrate and topped with vermiculite. They are 2 1 gallon, 2 1/2 gallon and 2 quart tupperware containers. I used the microwave to sterilize them (10 minutes each). I let them cool overnight and then used the oven tek to innoculate. I used a syringe I had previous used for spores, filling it with boiling water squirting it out several times and then torching the needle with a lighter. I let the needle cool and then filled the syringe from the jars of liquid mycelium after shaking the jars (Perhaps I should not have shaken them?). I injected between 2.5-10cc in each hole (5 holes per container) depending on the size (2.5cc for 2 quart, 5cc for 1/2 gallon, 10cc for gallon). It has been 3 days, now, and I am not seeing any mycelial growth in the containers (which are sitting in an incubator that fluctuates between 83-86 degrees).

Should I just keep waiting? Should I re-innoculate without shaking the jars, making sure I get more mycelium in the syringe? The only thing that I can think of is that I somehow killed the mycelium between the jars and the containers - perhaps by shaking.

Any help appreciated.

Thanks
-Asher


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Offlineahaig
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Registered: 10/15/01
Posts: 13
Last seen: 15 years, 6 months
Re: Honey Water Tek Innoculation - Slow/No Growth? [Re: ahaig]
    #608517 - 04/15/02 12:36 AM (16 years, 1 month ago)

I went ahead and re-innoculated one of the T3 2 Quart containers. I didn't shake the mycelium and got most of it into the syringe.

See how that goes, I guess....


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OfflineParticleMan
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Registered: 02/05/02
Posts: 240
Last seen: 16 years, 27 days
Re: Honey Water Tek Innoculation - Slow/No Growth? [Re: ahaig]
    #608601 - 04/15/02 02:37 AM (16 years, 1 month ago)

the first million times i did it, i had bacteria....


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OfflineSIR_JAS
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Registered: 03/19/02
Posts: 22
Loc: kansas
Last seen: 16 years, 29 days
Re: Honey Water Tek Innoculation - Slow/No Growth? [Re: ahaig]
    #609236 - 04/15/02 06:24 PM (16 years, 1 month ago)

dude i did the same thing with making dextrose water syringes....azure and PR...it took like a month for both jars to have good mycelim...and I innoculated like 12 qt brownrice/verm jars...only 2 eventually colonized, and come to find out my azure syringe was completely contaminated thanxs to sporeworks...(and they have yet to respond to my polite emails)....prick asses


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Amazon Shop: ½ Pint Jars, 10cc Syringe, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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