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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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What is the number one cause for contamination?
    #5990410 -

Does contamination usualy happen during PC'ing/cooling of jars or when injecting with spores? I want to make sure I do this right the first time so I plan to make a glove box, alcohol lamp, and turn my bathroom into a clean room for inoculation but I just hope my cheapo PC didnt screw me up.

So what is the number one cuase for contamination?

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Re: What is the number one cause for contamination? [Re: TrippinOnAcid]
    #5990445 -

inoculation.

pc'ing correctly kills all the bad guys.

having a poor sterile practice during inoculation,
or a wet filter (verm layer or tyvek, poly-fil,...)

if you have a glove box, you don't need a "clean room"

I think besides poor inoculation, these are some major no no's:


incubating jars at too high of a temperature
incorrect moisture content inside the jars
incorrect filter arrangement on jar

any cool needle exposed to open air can transmit contams.
either keep your needle hot or covered in something soaked in isopropyl.

a glove box is a great idea, do some homework and make it right.

good luck.

later on, stale air, poor water delivery and other things also come into play.


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Re: What is the number one cause for contamination? [Re: monstermitch]
    #5991252 -

You as the cultivator are the number one cause of contamination.

Our mouths are full of bacteria and thousands escape with each breath. Our fingernails and exposed skin are teeming with bacteria. Our clothing is covered with the mold spores we've picked up from walking around, and the same holds true for our hair.

The PC is rarely the problem if used properly. Contaminants don't care if they're killed by 15psi from a cheapie or an All American.

For best results, build a glovebox to work in until you get into the hobby enough to justify a laminar flow hood. Bathrooms are FULL of black mold and fecal material/bacteria.

Use gloves and wash them with alcohol after you put them on. Flame sterilze your needles and use them while still hot. The first drop or two of solution will cool the needle, allowing the rest to flow uncontaminated. Flame the needle between jars to prevent cross-contamination, but you don't need to flame between holes in the same jar. If you're working in a glovebox, a hair net and surgical mask are not necessary, but if working in front of a flowhood, be sure to use both. Spray the air with Oust or a very fine mist of 10% bleach(which will ruin your clothes and carpet), and clean your work surfaces and tools with alcohol. If something MUST be exposed to the air, make sure it's for the minimum amount of time possible. Plan ahead so you have everything you need when the work starts. Good luck.
RR


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Re: What is the number one cause for contamination? [Re: RogerRabbit]
    #5991319 -

I made a poor mans box. I got a large box cut off the top and put saran wrap over the top taped the sides of it and all the creases made to arm holes that we some what tight but left a little room . I put everything in the box lysol, alcohol, lighter, syringes, precut foil etc. I didnt use gloves but i did spray the hell outta my arms/hands and the inside of the box leaving my arms inside the box and waited bout 30 sec with leaving the hands inside and inocculated. Of course i did the common sense stuff and flamed and alchol the needle (i know over kill) between each jar and had no contams. I gave a liberal spray of the box before putting the wrap on the top. make sure you cut the arm holes in the right spot cause it can get uncomforable if you dont.


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The are no stupid questions, only stupid people, now point and laugh every one.

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Re: What is the number one cause for contamination? [Re: firstcut]
    #5991499 -

I rarely have contaminations inside the jar. What gets me is the casing layer after a spawn run, when I put the trays in their fruiting chamber (Martha setup with one coolmist entering through 3 holes). And the contaminations are always cobweb followed by trich. The cause is due to the humidty being too high (95%+) with a lack of air exchange or stale air pockets within the container. If you notice it early, hydrogen peroxide usually does the trick.

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Re: What is the number one cause for contamination? [Re: TrippinOnAcid]
    #5993614 -

Yeah, don't get any of your cooties in the mix or you might grow tiny evil clones that will try to climb in your mouth and reproduce via mitosis. Then you will have to shoot them in the face and chop them up and make sure to avoid the necronomicon.

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Re: What is the number one cause for contamination? [Re: TrippinOnAcid]
    #5994364 -

if you believe that your PCing or boiling is the culprit for your contam issues, you can leave 1 (or all of the jars) uninnoculated for a week or so to see if they contam. if they don't, then you know that's not the problem


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please do not take everything i say here so personally. welcome to teh internets!

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Re: What is the number one cause for contamination? [Re: Optx]
    #5994594 -

my trouble comes from either

bad lc or spore print
green mold on casing after 2nd flush

i would not consider myself to be very sterile
i never wear gloves or a mask
usually 90-95% sucess rate\

i built a glovebox for my first few grows but it was so much of a pain to use i figured i could use that wasted time to do more jars and i still come out ahead.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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