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Offlinematt_420_
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Registered: 10/22/01
Posts: 29
Loc: VA
Last seen: 14 years, 9 months
best way to sterilize needle?
    #537668 - 02/01/02 11:33 AM (14 years, 10 months ago)

i was wondering what sort of precedures others were using for sterilizing there syringe? should i go so far as to put up a shower curtain around my working area? any help is greatly appreciated!

**Explore yourself to your deepest fear; after that, fear has no power, and the fear of freedom shrinks and vanishes. you are free***


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Offlinetomatofarmer
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Registered: 01/10/02
Posts: 261
Last seen: 14 years, 9 months
Re: best way to sterilize needle? [Re: matt_420_]
    #537676 - 02/01/02 11:44 AM (14 years, 10 months ago)

all i did was this on my first set of jars.
i turned oven on to warm
pulled rack out
sprayed lysol(and of corse u have santized your area with lysol and alcahol)
i flamed my needle with a lighter till red hot
then i wiped it with alcahol to cool it
i injected my jars 4 holes. i put syringe strait through tape on holes
i resterlized with lighter and alcahol every jar to avoid cross contamination
i did not retape the holes
i covered back with foil
then i put in incobation chamber(aquar. heater set up)
and that was like 8 or 9 days ago.
i got my first pins today on one jar of lipas and the pesas are not far behind
Peace and may the force be with you
-Lee


--------------------
I swear those mushrooms are going on my pizza officer!



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Offlineiangato
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Registered: 01/30/02
Posts: 220
Loc: maine, USAma
Last seen: 14 years, 7 months
Re: best way to sterilize needle? [Re: matt_420_]
    #537677 - 02/01/02 11:44 AM (14 years, 10 months ago)

hola matt, what i usually do is i sterilize the needle by makin it red hot with an alcohol lamp or a butane lighter. i find the easiest way to inoculate is by turning the stove on and pulling out the bottom rack as far as it will come out without falling out. use this as your platform to set the jars. vthe heat from the over should keep all the bad contams away. hope i helped. adios. peace.


--------------------
a blurry dot dances among the shadows
bends the light
and fizzles into my pink and glowing mind

-ian gato


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Anonymous

Re: best way to sterilize needle? [Re: matt_420_]
    #537694 - 02/01/02 12:03 PM (14 years, 10 months ago)

Your needle should already be sterile if it's coming out of an unopened pacakge.

And after innoculating the first jar, it should still be sterile. After all, your jar shouldn't have been contaminated so neither should your needle.

All this flaming and rubbing with chemicals, its a bunch of overkill and shouldn't be needed. It only takes a bit of common sense to be working in a clean area.


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Invisibleralphster44
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Registered: 01/04/01
Posts: 4,657
Re: best way to sterilize needle? [Re: matt_420_]
    #537718 - 02/01/02 12:35 PM (14 years, 10 months ago)

Post deleted by administrator.


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OfflineUnder_net
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Registered: 12/09/01
Posts: 228
Loc: kentucky
Last seen: 13 years, 10 months
Re: best way to sterilize needle? [Re: ralphster44]
    #537884 - 02/01/02 04:37 PM (14 years, 10 months ago)

same here
you can get a pack of 100 alcohol wipes for a couple few bucks


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Anonymous

Re: best way to sterilize needle? [Re: ralphster44]
    #537887 - 02/01/02 04:43 PM (14 years, 10 months ago)

Agreed. I recommend inoculating over a warm oven door, and make sure the isopropyl alcohol you use to wipe the needle is at least 91%. Peace.


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Anonymous

Re: best way to sterilize needle? [Re: ]
    #537959 - 02/01/02 06:18 PM (14 years, 10 months ago)

Actually 70% - 80% alcohol is more effective at killing germs. The higher percentages make for better flames...


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Invisibleangryshroom
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Registered: 12/18/01
Posts: 7,262
Re: best way to sterilize needle? [Re: tomatofarmer]
    #537965 - 02/01/02 06:27 PM (14 years, 10 months ago)

Tomato Farmer...

You got pins within 9 days from inoculation? How long did it take for your jars to colonize?


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Invisiblear393
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Male
Registered: 01/27/00
Posts: 696
Loc: NYC, NY, US
Re: best way to sterilize needle? [Re: angryshroom]
    #538495 - 02/02/02 12:31 PM (14 years, 10 months ago)

actually 70% is the best (due a few properties of all alcohols) for cleaning/killing.
peace, ar393


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OfflineNorthernsoul
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Registered: 11/17/01
Posts: 2,290
Loc: Inner Eye
Last seen: 5 years, 11 months
Re: best way to sterilize needle? [Re: matt_420_]
    #538524 - 02/02/02 01:07 PM (14 years, 10 months ago)

When using the oven tek, and if you have a gas oven, just turn on the front left or right burner and youll get a nice hot flame to sterilise the needle with quick. Wipe with alcohol to cool. Im a clean freak and heat the needle after every jar.


--------------------


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OfflineNorthernsoul
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Registered: 11/17/01
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Re: best way to sterilize needle? [Re: tomatofarmer]
    #538527 - 02/02/02 01:12 PM (14 years, 10 months ago)

then i put in incobation chamber(aquar. heater set up)
and that was like 8 or 9 days ago.
i got my first pins today on one jar of lipas>>>>>>

Wow, Ive heard that those lipas colonise quick, I have some B+brf jars and its been 9 days and theyre only 25%..and .theyre at 85degrees 24/7


--------------------


--------------------------------------------------------------

When it comes
I'll know, I know
Just take my clothes and leave
And I'll be gone




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Offlinematt_420_
newbie
Registered: 10/22/01
Posts: 29
Loc: VA
Last seen: 14 years, 9 months
Re: best way to sterilize needle? [Re: Northernsoul]
    #538588 - 02/02/02 02:32 PM (14 years, 10 months ago)

those suggestions helped greatly, i wiped with alcohol and heated before every jar over the stove; i innoc yesterday, but ive been wondering how much moisture should be in the jars? mine dont have any! peace

Even castles made of sand, fall into the sea, eventually


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Offlinemoxjet20
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Registered: 07/25/01
Posts: 160
Loc: santa cruz, magical land ...
Last seen: 14 years, 6 months
Re: best way to sterilize needle? [Re: tomatofarmer]
    #538731 - 02/02/02 06:19 PM (14 years, 9 months ago)

might want to be careful spraying lysol near an open flame (i.e. the oven)!!


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Offlinecookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 14 years, 7 months
Re: best way to sterilize needle? [Re: Northernsoul]
    #538864 - 02/02/02 10:05 PM (14 years, 9 months ago)

I had B+, thai, australian, and equador
eq got contam, thai got contam, aust didnt do anything, B+ took off like a son of a who re
all on PDA agar
inoculated some more pitres with the 3 that didnt do so well the first time.
Funny thing iwht them though, they worked the very besdt in the brf jars

oh well.

B+ is a fast mother fucker when it gets going on agar
like 2 days, and a .5x.5cm slab of agar will colonize a new pitre. if placed in the middle.


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