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InvisibleMr. G
journeyman
Registered: 04/24/99
Posts: 46
Loc: Treasure Coast
Glovebox for $20.00 US or less!
    #616862 - 04/23/02 11:56 PM (14 years, 10 months ago)

How to make a wet box, or a "glovebox with alcohol bath" for $20.00 or less.

This is by far the easiest and cheapest way to make a box for working with spores, cultures, clones and other specimens. This will insure that your slides are clean and proper. It is very easy and cheap. Here is what you need.

1 - 10 gallon Aquarium, $9.00 Wal-Mart.
1 ? small sheet of Plexiglass, Ace Hardware $4.00
1 ? tube clear Silicon Sealer, Ace or Wal-Mart $3.59
1 ? Utility Knife with sharp blade $4.00 (probably available already, go buy new blades)

Cut the sheet of Plexiglass down until it will fit snuggly in the top of aquarium. Most 10 gallons have a lip that goes around the inside that is recessed. Cut the Plexiglass so it fits perfectly inside the aquarium, but covers the entire top and rests on the inside lip. Then remove the Plexiglass and cut a square out of the top corner on each side of one of the longer sides. It makes no difference which side at this point, as you are going to be laying the Aquarium on it side. You will be working with the Aquarium on its side, with arms inserted in the left and right hand bottom corners. These holes should be big enough that you can easily fit your hand and arm into them. You can make one hole bigger then the other if you prefer, (for inserting plate with specimens on it into the Aquarium or Sterile Area) the floor is going to be a pool of alcohol. Make sure that you make one "cut out" big enough you can fit a small plate or glass bowl into the Aquarium with the plexiglass in place. You can keep the hole smaller if you tilt the plate, and place the plate inside the Aquarium by pushing through the hole catty cornered. Then place your specimens in the Aquarium and lay on the plate. Save your trash strip (excess Plexiglass), you will need it for a cover later. With the Aquarium in the normal position, go ahead and run a bead of silicon around the top lip inside where the plexiglass is going to rest. Lay the plexiglass down onto the siliconed area. When it is in place, go ahead and seal the entire outside edge of, but an extra bead from the outside assures it is sealed properly. One silicon bead would probably be enough, but this is to be extra sure you are sealed. The holes you cut (left and right) need to be on the same side in each corner. Let the tank dry the amount of time the silicon recommends. I would allow at least overnight.

Lay the aquarium on the side with the holes cut into it. Your hands and a small "working plate" or bowl should easily fit into holes if you cut correctly. Take the lids from two alcohol bottles and glue in each corner of the Aquarium, on what would normally be the outside near the top. This is the side that it is resting on after you lay it down. You can put a dab of silicon on the lid, lift the Aquarium and place alcohol bottle lid on each side as close to front as possible. I would glue to the glass, not the trim strip around the Aquarium. This will make the Aquarium be on its side, with a tilt toward the back. This will allow you to have a bigger pool of alcohol on the bottom. When the lids that tilt it backwards are dry, we are ready to start.

To work:

Pour in two bottles of rubbing alcohol. This should fit easily. If it doesn?t, save the excess for later use. You just need the pool of alcohol to cover the entire side it is resting on, from what would have been the top of the tank to the bottom if it was not on it?s side. You are now ready to work. The extra plexiglass piece should easily cover the holes by standing on its side and propping against the Aquarium when not in use. The natural evaporation of the alcohol will force an outward flow of air. This will keep it sterile, and the alcohol fumes will prevent bacteria, spores, etc. from entering. If you hav ae HEPA already, blow the beam of air across the front after placing specimens in the box. This will create a "force field" of air exactly like a flow hood, with the added protection of evaporating alcohol and the fumes helping to keep outside air out, and the inside sterile. If you are working, and the top or the piece you are looking down through fogs up, simply squat, and you can see perfectly through the clear lid that is glued in place. If you don?t have a HEPA, don?t run out and buy one, it is not necessary and you will have very low contamination rates from contamination occurring in the box. It works miracles people, and it is cheap and easily stored. I like it because you can actually open a Petri Dish that has a small area of mold, cut the mold out, drop into the alcohol and pick up and remove after you are done. When finished with the moldy one, you DO NOT have to clean and resterilize the entire box. The fumes kill or flush out and kill escaped spores. You are ready to work again with no problem. You can use common sense and clean and wash as necessary, but you could actually allow it to get kind of grungy and still have sterile conditions, as long as you check and refill the alcohol as necessary.

We always use gloves and a face mask, when working in the box or with trays or casing. Just using the gloves and mask will help you to eliminate a tremendous amount of contamination. With the combination of the two and good common sense, you will do extremely well. Mr.G

Dictated 5/12/96 by Mr.G, property of Foggy Mountain Farms International. Typed by Ann B.


--------------------
"Mr.G with a rose, in and out of the garden goes, country gharden in the wind and the rain whereever he goes the people all complain!"
"The Grateful Dead" Thanks boys, I miss you Jerry!
Did it hurt that much?


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OfflineTeRzMaStA
Psilocybin Pimp

Registered: 10/08/01
Posts: 1,157
Last seen: 4 days, 6 hours
Re: Glovebox for $20.00 US or less! [Re: Mr. G]
    #616876 - 04/24/02 12:09 AM (14 years, 10 months ago)

can you post a couple of pictures?


--------------------
Think for yourself; Question Authority


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OfflineDoublethink
lost cause
Male
Registered: 03/27/02
Posts: 182
Loc: PDX, OR
Last seen: 4 years, 3 months
Re: Glovebox for $20.00 US or less! [Re: Mr. G]
    #616884 - 04/24/02 12:21 AM (14 years, 10 months ago)

This sounds sweet! I wanna see pics!


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OfflineDoublethink
lost cause
Male
Registered: 03/27/02
Posts: 182
Loc: PDX, OR
Last seen: 4 years, 3 months
Re: Glovebox for $20.00 US or less! [Re: Mr. G]
    #625722 - 05/03/02 02:59 PM (14 years, 9 months ago)

Please post pics!


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