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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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I have tried everything and still cant get one pin and contamination
    #5733981 -

Hey guys, Well I just dont know what to do anymore. I have tried to grow for the past 4 months with no luck I have bought pressure cookers, the martha stewART CLOSET THING that zips up, a vicks cool humidifier plus another one i cant remember the name, 2 hepa air cleaners with one I attached to a glove box, I tried Birdseed tek, rye, Pf, I bought jars on the web tried to make my own. etc, etc,. I know most are thinking like me saying,"you got to be doing something wrong", I just can t figure out what it is. I am wondering if it is maybe my house. My wife thinks maybe I am trying to hard the closest I have come is getting a a couple to jars to fully colonize but they are so dry , and after I did the dunking nothing happens. and yeah , I have thermometer/hydrometers. has anyone had the similar problems and found a sure fire way to get them to grow. MOST OF MY CONTAMINATION is either blue green or the white fuzzy, and sometimes wet spot. I also get my spores from reputable places (I guess) that i have seen on here. Any help or comments except for blasting me saying stupid sh*t is appreciated. BTW I live on east coast near NYC

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5733992 -

have you made liquid cultures?

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Re: I have tried everything and still cant get one pin and contamination [Re: Atheist]
    #5734079 -

I'll bet the problem is the hepa connected to your glovebox. Use a still air glovebox and watch your problems go away.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734123 -

Like in a solution of malt? Yeah I think I tried once maybe but not enoughto cancel that out as an option. what do you suggest

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Re: I have tried everything and still cant get one pin and contamination [Re: RogerRabbit]
    #5734150 -

RogerRabbit said:
I'll bet the problem is the hepa connected to your glovebox. Use a still air glovebox and watch your problems go away.
RR


Or a flowhood. I'm building one, and I think it's worth the investment (maybe 150$ if you build it yourself and buy everything new).

Quote:
garygenn69
Like in a solution of malt? Yeah I think I tried once maybe but not enoughto cancel that out as an option. what do you suggest



Yeah, if you grow mycelium in a culture you can isolate a nice rhizomorphic strain to use and insure optimal fruiting. I think not as many people use liquid cultures these days with the PF Tek and its derivatives, but it's probably the most interesting way from a mycological perspective. If you choose this route you'll need some good books though; I reccommend "The Mushroom Cultivator" by Paul Stamets and " Psilocybin Mushroom Handbook: Easy Indoor & Outdoor Cultivation" by L.G. Nicholas and Kerry Ogame.

You seem to either have a REALLY messy apartment or just bad luck, but I think if you build a flowhood and be extra careful with sterilizing ALL of your equipment you should be good. Some H2O2 helps too.

Good luck and happy growing =)


--------------------
Do what thou wilt shall be the whole of the law
Love is the law, love under will
--Frater Baphomet

Edited by va_shroomer (06/10/06 02:41 PM)

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734152 -

why not just clean your workroom really well and do open air innoculations? It sounds like you went waaaaaaay overboard.


--------------------
channel your inner Larry David

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734159 -

well...

your blue/green whatever is either bruising from you cakes being too dry or it is mold.

If it is mold, your inoculation technique is bad.
RR is very correct, a glove box is a still air environment if done correctly.
disconnect that hepa and plug up all holes.
The air in your glove box is to be still (not moving at all)

the wet spot is either from not pc'ing at 15psi for 75-90 minutes or incubating over 84 degrees.
Don't incubate over 82 degrees.

PF, WBS, Rye, it is your choice.

Sounds to me like your problem is overwhelmingly your sterile techniques.

Pressure cook for 75-90 minutes at 15psi.
Study up on clean inoculation techniques.
Don't incubate your jars at a temp over 82 to be safe.

The white stuff is the good stuff, not a contam.

Try again.
Don't continue to do the same thing expecting a different result.


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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734165 -

yeah thanks , I sweAR I FEEL LIKE I HAVE TRIED to clean it well. actually the best results(meaning the closest I have ever gotten to fruiting) was cleaning with bleach instead of lysol and and that air cleaning stuff. Thanks for replying

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734171 -

http://www.shroomery.org/forums/showflat...rue#Post5368509

http://www.shroomery.org/forums/showflat...rue#Post5296250

http://www.shroomery.org/forums/favlinke...r=1&postmarker=

some words from agar that will probably help.


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Re: I have tried everything and still cant get one pin and contamination [Re: monstermitch]
    #5734248 -

One of the major sources of bacterial contamination is the breath of the cultivator. Never talk or even exhale when doing inoculations, even if you have a flowhood. Wear a surgical mask. Wear gloves and wash them with alcohol after putting them on. These are the precautions I take when using a laminar flow hood. You can't be too sterile. Failure is a lot more frustrating than the few extra steps to avoid it.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: I have tried everything and still cant get one pin and contamination [Re: RogerRabbit]
    #5734296 -

Yeah you can never be too cautious. Friggin stamets does inoculations with no glove s and no mask and he has them hairy ass arms and hands plus that beard has to trap all kinds of mold spore carrying dusts.

He says he doesn't like the restricted movement glove cause so he just cleans his hands with alcohol.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Re: I have tried everything and still cant get one pin and contamination [Re: Psychoslut]
    #5734322 -

He also has an entire wall of flowhoods three feet tall, but 20 of the 30 test tube cultures I got at the masters seminar contaminated before I even opened them the first time a year later. He knows better.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734352 -

thank you!

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Re: I have tried everything and still cant get one pin and contamination [Re: RogerRabbit]
    #5734356 -

:wtf: flowstone could make better cultures than that


ill bet its his retarded staff that does it. Ive heard nothing but bad news from fungiperfecti products, the bags barely produce etc. etc.


I highly doubt stamets personally makes everything.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

Edited by Psychoslut (06/10/06 03:37 PM)

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734364 -

Man, I swore I read somewhere to have clean air blowing through, but that makes sense if the air is not moving no contams can hop on the syringe.. I know I am pc'ing long enogh. I bough one of the i think 12 quart prestos it says if the top is moving (rocking back and forth) that is the proper psi. thanks again for replying

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734373 -

get some Oust and lots of it

the white can, unscented :thumbup:

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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
    #5734409 -

garygenn69 said:
Man, I swore I read somewhere to have clean air blowing through, but that makes sense if the air is not moving no contams can hop on the syringe.. I know I am pc'ing long enogh. I bough one of the i think 12 quart prestos it says if the top is moving (rocking back and forth) that is the proper psi. thanks again for replying


you did read that somewhere.
not everything written is correct or fool-proof.

live and learn.

you'll have much better success with still air.


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Re: I have tried everything and still cant get one pin and contamination [Re: monstermitch]
    #5737195 -

forget gloveboxes, waste of time and effort. oven tek is the boooomb. never had one contamination out of 30, try to see the germs in the air and your procedures will improve, gloves and mask a must they keep your hands from burning which can cause sudden movements which in turn doesn't allow the heat to do its job

Edited by PsyCode (06/11/06 06:21 AM)

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Re: I have tried everything and still cant get one pin and contamination [Re: PsyCode]
    #5737199 -

sorry dude but I have to disagree...


the oven tek is crap...

your just having some good luck!~


nothing beats a still air box, glove box or a flowhood!~



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!

Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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Re: I have tried everything and still cant get one pin and contamination [Re: Roadkill]
    #5737215 -

I really don't think it's luck...lol. I was chosen for this by the shroom god as you all, well, most of you. I just think the effort involved won't work well with a newbie. I heard more problems with boxes than the oven tek. The flowhood, yeh I agree, but the oven tek is very similar and with care it works fine. I also use a rice cooker to pc with 17 psi, works every time.

Edited by PsyCode (06/11/06 06:45 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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