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Anonymous
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 Innoculation and Sterilization HELP! 
#1715914 - 07/14/03 08:10 PM (20 years, 4 months ago) |
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Hi, I will be inoculating tomorrow so I need as much information as I can get right now. Will I need lysol? or can I just use rubbing alcohol to wipe everything down and take a shower and wear clean cloths etc. and inoc. using a syringe into 4 holes in the lids with a layer of tin-foil underneath and on top I boiled the jars for 1.5 hours in a pot and added water during the boiling process steam was constantly escaping the lid is this ok? or is the steam supposed to be help in during the entire process?
Thanks any information will be appriciated
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 LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: ]
#1715941 - 07/14/03 08:16 PM (20 years, 4 months ago) |
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Post deleted by administrator
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1715971 - 07/14/03 08:20 PM (20 years, 4 months ago) |
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Thanks KuSsH you have replied to almost all of my posts on this board. Do you really need to wipe every time you squirt 1\4 mL of spore solution into each hole? And would it be ok if I let my jars sit after boiling them for more than a day like 2-3 days? Also would it be just as effective to use rubbing alcohol or hydrogren peroxide instead of bleach or is bleach better? What should I wipe with bleach? working area? syringe? jars? And last of all the bleach will not harm anything in the jar even if the needle is inside of it with wet bleach still on it?
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: ]
#1716012 - 07/14/03 08:32 PM (20 years, 4 months ago) |
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716051 - 07/14/03 08:41 PM (20 years, 4 months ago) |
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So what do you use to wipe the bleach with? a old cloth?
...This might be just a little off topic but whats the average size of a hygrometer?
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 kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: ]
#1716057 - 07/14/03 08:42 PM (20 years, 4 months ago) |
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Bleach with paper towel? Alcohol evaporates and leaves water, so no need to wipe.
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716062 - 07/14/03 08:43 PM (20 years, 4 months ago) |
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Wow... 3 second reply
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716063 - 07/14/03 08:44 PM (20 years, 4 months ago) |
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average size of hygrometer... analog like fifty cent JFK coin. Digital like pack of smokes...
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716070 - 07/14/03 08:45 PM (20 years, 4 months ago) |
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Damn that one was like 4.2341 seconds
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: kukusz]
#1716073 - 07/14/03 08:46 PM (20 years, 4 months ago) |
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Post deleted by administrator
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716083 - 07/14/03 08:48 PM (20 years, 4 months ago) |
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So do you guys wear gloves when you do this isnt bleach corrosive? how do you get the bleach on the paper towel?
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: ]
#1716090 - 07/14/03 08:49 PM (20 years, 4 months ago) |
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Post deleted by administrator
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716101 - 07/14/03 08:51 PM (20 years, 4 months ago) |
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Lol ya they are pretty easy -_- ... sorry watched Dragon (the Bruce Lee movie) today.
BTW you didnt really answer my question do you use gloves or is it just a good idea?
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716104 - 07/14/03 08:52 PM (20 years, 4 months ago) |
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Get gloves at Cosco or Sams or Price Club... 2 Boxes of 100 (that's 100 total pairs for the geniuses) for under $10. Or get small zip loc baggies in bulk, and use those as ghetto gloves.
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: kukusz]
#1716111 - 07/14/03 08:54 PM (20 years, 4 months ago) |
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Post deleted by administrator
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charvo
JOURNEYMAN
Registered: 03/09/03
Posts: 654
Loc: In The Hand Of The World
Last seen: 9 years, 7 months
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716117 - 07/14/03 08:54 PM (20 years, 4 months ago) |
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if your at the point of knocking up your jars and your not exposing the grain to the air,what is the point in being so clean now all you have to do is shoot those jars up.am i missing something here.hygene is important in ones life but i think to be that clean just shoot up jars is just the wroug time to worry about it.
i would just make sure that needle is clean and blast your load in them jars good luck man.
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716120 - 07/14/03 08:55 PM (20 years, 4 months ago) |
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Hmmmmmm... Ghetto glove huh... again the gloves are only for wiping down with bleach? or should I wear these "ghetto gloves" while im actually innoculating... Damn I could just picture that ugly picture right now 
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: ]
#1716141 - 07/14/03 08:59 PM (20 years, 4 months ago) |
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I wasn't reading the top of the post... my friend uses gloves (ghetto or not) when handling cakes in a terrarium, for harvesting, etc. When innoculating, he never uses anything, since he is only touching thing like syringes and jars. But this might not be considered the safest way.
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716171 - 07/14/03 09:06 PM (20 years, 4 months ago) |
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So basically I should just wipe the work area down with bleach spray the room down with lysol give it a good minute to settle, light the syringe till red hot wipe it with bleach innoc. a hole , repeat?
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: ]
#1716177 - 07/14/03 09:07 PM (20 years, 4 months ago) |
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I would not bother with wiping post flaming the syringe to red hot. You killed _everything_ flaming the needle, wiping it just reintroduces chance of contamination.
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: kukusz]
#1716186 - 07/14/03 09:09 PM (20 years, 4 months ago) |
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Post deleted by administrator
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716193 - 07/14/03 09:11 PM (20 years, 4 months ago) |
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Hmm... So should I use bleach or alcohol?... Or wait a minute maybe both? 
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716201 - 07/14/03 09:12 PM (20 years, 4 months ago) |
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but what is the point? I could see some poor new guy flaming and alcoholing his needle until the metal is worn down... I don't think that using alcohol on the needle post flaming is required. I do agree that it probably won't introduce any new contams, but you think it's required?
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716206 - 07/14/03 09:12 PM (20 years, 4 months ago) |
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lol just burn your house down!! Veeeeeeeeery sterile, kill everything.
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: kukusz]
#1716226 - 07/14/03 09:15 PM (20 years, 4 months ago) |
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Post deleted by administrator
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716249 - 07/14/03 09:19 PM (20 years, 4 months ago) |
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Well, at first I was planning on just heating the needle till red hot every 2 jars. But LiL KuSsH speaks otherwise and I would trust someone with experience than my crazy beetch of an ass.
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: ]
#1716262 - 07/14/03 09:21 PM (20 years, 4 months ago) |
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Post deleted by administrator
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716287 - 07/14/03 09:26 PM (20 years, 4 months ago) |
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I was referring to me as crazy but if it makes you feel better you can be crazy too
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: ]
#1716292 - 07/14/03 09:27 PM (20 years, 4 months ago) |
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Does anyone *looks at KusSH* know where I can find a good dunk tek.
I thought there was one in the FAQ before but after a long hard desperate 2 minute look I failed miserbly
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: ]
#1716297 - 07/14/03 09:28 PM (20 years, 4 months ago) |
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the dunk tek here is not really necessary if you have 1/2 pint jars...
http://www.mycotopia.net/teks/dunktek.html
but I think it's what you are talking about.
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: kukusz]
#1716303 - 07/14/03 09:30 PM (20 years, 4 months ago) |
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Post deleted by administrator
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
#1716329 - 07/14/03 09:37 PM (20 years, 4 months ago) |
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K , thx kukusz, but I still have a few questions that I hope can be answered, would it be ok to use a freezer? ( the long big ass kind that sits on the floor) and if I were to do it this way, I would probably keep submerged for 6-12 hours. Also the method that says you put the cakes in jars and submerge in water, would you be able to just use the jar they are colonizing in? Just say Im about to birth my cakes I fill the jars they are sitting in with cold water refridgerate or put somewhere cool ( my friend has AC and his room is in basement should be cool enough) and just let them sit than when they are ready to be birthed I just take them out as I empty the water? And another question these cakes would float if nothing was holding them down am I correct?
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: ]
#1716342 - 07/14/03 09:40 PM (20 years, 4 months ago) |
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I would not freeze them, very bad. 6 hours with that little water and they will be ice-cubes. Yes, just use your jars that they are colonizing in. Putting them in a cool place other than fridge will work just fine. 24 hours....
Yes they would float, put the lid on 
And yes, just dump the water out of the jar (using the lid to keep the cake from ending up in your garbage disposal) and stick in fruiting chamber. Do not bother wiping the cake dry like some suggest, it will evaporate in like 30 minutes.
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
Edited by kukusz (07/14/03 09:43 PM)
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lysergic
Mycophile!
Registered: 06/09/03
Posts: 691
Last seen: 20 years, 10 days
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716355 - 07/14/03 09:42 PM (20 years, 4 months ago) |
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personally I'd recommend using an inoculation hood that I detail making in the post here http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum2&Number=1627128&fpart=&PHPSESSID=
I guarantee you if you use this, you will slice your contam rate down DRASTICALLY, or I give you a 100% cash refund!
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In response to an attack killing 15 American Servicemen
PsiloKitten said:
Just give em a little more time, the iraqis are making great progress. And this is unorganized. Wait till they get organized.
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: lysergic]
#1716362 - 07/14/03 09:45 PM (20 years, 4 months ago) |
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KusSH would you recommend I wait to get lysol before innoculation or just procede with bleach and flaming the syringe.
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kukusz
Owner of belowhot monkey!
Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 13 years, 11 months
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Re: Innoculation and Sterilization HELP! [Re: ]
#1716373 - 07/14/03 09:48 PM (20 years, 4 months ago) |
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Oh so you gotta ask KuSsh, huh? Pffffffttt...
my friend never used lysol ever, but it probably is safer...
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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.
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Anonymous
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Re: Innoculation and Sterilization HELP! [Re: kukusz]
#1716379 - 07/14/03 09:49 PM (20 years, 4 months ago) |
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Lol, sorry kukusz thanks for your help and opinions
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Deleted [Re: ]
#1716393 - 07/14/03 09:51 PM (20 years, 4 months ago) |
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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amazonherbman
Stranger
Registered: 07/14/03
Posts: 3
Last seen: 19 years, 11 months
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 Re: Peroxide and HoneyH20 [Re: LiL_KuSsH]
#1717862 - 07/15/03 10:32 AM (20 years, 4 months ago) |
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I need to find out what the ratio for injecting peroxide into honey water spawn. would you use 1-2cc straight into 1 pint or like 5cc of a 1:9 mix???
any advice would be greatly appreciated
ps. the H202 kills spores.. right, but does it also kill growing contams?
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LiL_KuSsH
Lost In The Trip
Registered: 05/07/03
Posts: 3,001
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Post deleted by administrator
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amazonherbman
Stranger
Registered: 07/14/03
Posts: 3
Last seen: 19 years, 11 months
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Re: Peroxide and HoneyH20 [Re: LiL_KuSsH]
#1721201 - 07/16/03 10:16 AM (20 years, 4 months ago) |
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thanks,
What i meant was a growing culture in honeywater, which might be at risk for contamination. adding peroxide to that ....
so the primary use would be to protect your mushies when fruiting - by adding it to a cool mist humidifier or similar setup right?
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cyndyl
mycologist wannabe
Registered: 05/26/03
Posts: 193
Loc: far away from home
Last seen: 12 years, 10 months
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Re: Innoculation and Sterilization HELP! [Re: ]
#1721231 - 07/16/03 10:34 AM (20 years, 4 months ago) |
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I've been using those new Chorlox bleach towelettes that come in a roll in a despenser so I don't spread the germs around with a dirty rag. I wipe down once, throw away towelette and wipe a second with another, then spray with 91% ISA.
Love those towelletes!
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There's no such thing as a stupid question,
but they're the easiest to answer!
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