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InvisibleLiL_KuSsH
Lost In The Trip
I'm a teapot

Registered: 05/07/03
Posts: 3,001
Deleted [Re: kukusz]
    #1716186 - 07/14/03 09:09 PM (20 years, 10 months ago)

Post deleted by administrator

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716193 - 07/14/03 09:11 PM (20 years, 10 months ago)

Hmm... So should I use bleach or alcohol?... Or wait a minute maybe both?  :shocked:

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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 14 years, 5 months
Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716201 - 07/14/03 09:12 PM (20 years, 10 months ago)

but what is the point? I could see some poor new guy flaming and alcoholing his needle until the metal is worn down... I don't think that using alcohol on the needle post flaming is required. I do agree that it probably won't introduce any new contams, but you think it's required?


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.

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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 14 years, 5 months
Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716206 - 07/14/03 09:12 PM (20 years, 10 months ago)

lol just burn your house down!! Veeeeeeeeery sterile, kill everything.


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.

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InvisibleLiL_KuSsH
Lost In The Trip
I'm a teapot

Registered: 05/07/03
Posts: 3,001
Deleted [Re: kukusz]
    #1716226 - 07/14/03 09:15 PM (20 years, 10 months ago)

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716249 - 07/14/03 09:19 PM (20 years, 10 months ago)

Well, at first I was planning on just heating the needle till red hot every 2 jars. But LiL KuSsH speaks otherwise and I would trust someone with experience than my crazy beetch of an ass.

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InvisibleLiL_KuSsH
Lost In The Trip
I'm a teapot

Registered: 05/07/03
Posts: 3,001
Deleted [Re: ]
    #1716262 - 07/14/03 09:21 PM (20 years, 10 months ago)

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716287 - 07/14/03 09:26 PM (20 years, 10 months ago)

I was referring to me as crazy but if it makes you feel better you can be crazy too

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: ]
    #1716292 - 07/14/03 09:27 PM (20 years, 10 months ago)

Does anyone *looks at KusSH* know where I can find a good dunk tek.
I thought there was one in the FAQ before but after a long hard desperate 2 minute look I failed miserbly

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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 14 years, 5 months
Re: Innoculation and Sterilization HELP! [Re: ]
    #1716297 - 07/14/03 09:28 PM (20 years, 10 months ago)

the dunk tek here is not really necessary if you have 1/2 pint jars...

http://www.mycotopia.net/teks/dunktek.html

but I think it's what you are talking about.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.

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InvisibleLiL_KuSsH
Lost In The Trip
I'm a teapot

Registered: 05/07/03
Posts: 3,001
Deleted [Re: kukusz]
    #1716303 - 07/14/03 09:30 PM (20 years, 10 months ago)

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716329 - 07/14/03 09:37 PM (20 years, 10 months ago)

K , thx kukusz, but I still have a few questions that I hope can be answered, would it be ok to use a freezer? ( the long big ass kind that sits on the floor) and if I were to do it this way, I would probably keep submerged for 6-12 hours. Also the method that says you put the cakes in jars and submerge in water, would you be able to just use the jar they are colonizing in? Just say Im about to birth my cakes I fill the jars they are sitting in with cold water refridgerate or put somewhere cool ( my friend has AC and his room is in basement should be cool enough) and just let them sit than when they are ready to be birthed I just take them out as I empty the water? And another question these cakes would float if nothing was holding them down am I correct?

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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 14 years, 5 months
Re: Innoculation and Sterilization HELP! [Re: ]
    #1716342 - 07/14/03 09:40 PM (20 years, 10 months ago)

I would not freeze them, very bad.  6 hours with that little water and they will be ice-cubes.  Yes, just use your jars that they are colonizing in.  Putting them in a cool place other than fridge will work just fine.  24 hours....

Yes they would float, put the lid on :smile:

And yes, just dump the water out of the jar (using the lid to keep the cake from ending up in your garbage disposal) and stick in fruiting chamber.  Do not bother wiping the cake dry like some suggest, it will evaporate in like 30 minutes.


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.

Edited by kukusz (07/14/03 09:43 PM)

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Offlinelysergic
Mycophile!
Registered: 06/09/03
Posts: 691
Last seen: 20 years, 6 months
Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716355 - 07/14/03 09:42 PM (20 years, 10 months ago)

personally I'd recommend using an inoculation hood that I detail making in the post here  http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum2&Number=1627128&fpart=&PHPSESSID= 

I guarantee you if you use this, you will slice your contam rate down DRASTICALLY, or I give you a 100% cash refund!

:smile:


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In response to an attack killing 15 American Servicemen
PsiloKitten said:
Just give em a little more time, the iraqis are making great progress. And this is unorganized. Wait till they get organized.

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: lysergic]
    #1716362 - 07/14/03 09:45 PM (20 years, 10 months ago)

KusSH would you recommend I wait to get lysol before innoculation or just procede with bleach and flaming the syringe.

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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/09/03
Posts: 519
Loc: East Coast
Last seen: 14 years, 5 months
Re: Innoculation and Sterilization HELP! [Re: ]
    #1716373 - 07/14/03 09:48 PM (20 years, 10 months ago)

Oh so you gotta ask KuSsh, huh?  Pffffffttt...

:tongue:

my friend never used lysol ever, but it probably is safer...


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716379 - 07/14/03 09:49 PM (20 years, 10 months ago)

Lol, sorry kukusz thanks for your help and opinions

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InvisibleLiL_KuSsH
Lost In The Trip
I'm a teapot

Registered: 05/07/03
Posts: 3,001
Deleted [Re: ]
    #1716393 - 07/14/03 09:51 PM (20 years, 10 months ago)

Post deleted by administrator

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InvisibleLiL_KuSsH
Lost In The Trip
I'm a teapot

Registered: 05/07/03
Posts: 3,001
Deleted [Re: LiL_KuSsH]
    #1716422 - 07/14/03 09:56 PM (20 years, 10 months ago)

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Offlineamazonherbman
Stranger
Registered: 07/14/03
Posts: 3
Last seen: 20 years, 5 months
Re: Peroxide and HoneyH20 [Re: LiL_KuSsH]
    #1717862 - 07/15/03 10:32 AM (20 years, 10 months ago)

I need to find out what the ratio for injecting peroxide into honey water spawn. would you use 1-2cc straight into 1 pint or like 5cc of a 1:9 mix???

any advice would be greatly appreciated
ps. the H202 kills spores.. right, but does it also kill growing contams?

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