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Anonymous

Innoculation and Sterilization HELP!
    #1715914 - 07/14/03 10:10 PM (13 years, 7 months ago)

Hi, I will be inoculating tomorrow so I need as much information as I can get right now. Will I need lysol? or can I just use rubbing alcohol to wipe everything down and take a shower and wear clean cloths etc. and inoc. using a syringe into 4 holes in the lids with a layer of tin-foil underneath and on top I boiled the jars for 1.5 hours in a pot and added water during the boiling process steam was constantly escaping the lid is this ok? or is the steam supposed to be help in during the entire process?
Thanks any information will be appriciated


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: ]
    #1715941 - 07/14/03 10:16 PM (13 years, 7 months ago)

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1715971 - 07/14/03 10:20 PM (13 years, 7 months ago)

Thanks KuSsH you have replied to almost all of my posts on this board. Do you really need to wipe every time you squirt 1\4 mL of spore solution into each hole? And would it be ok if I let my jars sit after boiling them for more than a day like 2-3 days? Also would it be just as effective to use rubbing alcohol or hydrogren peroxide instead of bleach or is bleach better? What should I wipe with bleach? working area? syringe? jars? And last of all the bleach will not harm anything in the jar even if the needle is inside of it with wet bleach still on it?


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: ]
    #1716012 - 07/14/03 10:32 PM (13 years, 7 months ago)

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716051 - 07/14/03 10:41 PM (13 years, 7 months ago)

So what do you use to wipe the bleach with? a old cloth?

...This might be just a little off topic but whats the average size of a hygrometer?


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 2 months
Re: Innoculation and Sterilization HELP! [Re: ]
    #1716057 - 07/14/03 10:42 PM (13 years, 7 months ago)

Bleach with paper towel? Alcohol evaporates and leaves water, so no need to wipe.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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Anonymous

Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716062 - 07/14/03 10:43 PM (13 years, 7 months ago)

Wow... 3 second reply


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 2 months
Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716063 - 07/14/03 10:44 PM (13 years, 7 months ago)

average size of hygrometer... analog like fifty cent JFK coin. Digital like pack of smokes...


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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Anonymous

Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716070 - 07/14/03 10:45 PM (13 years, 7 months ago)

Damn that one was like 4.2341 seconds


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: kukusz]
    #1716073 - 07/14/03 10:46 PM (13 years, 7 months ago)

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716083 - 07/14/03 10:48 PM (13 years, 7 months ago)

So do you guys wear gloves when you do this isnt bleach corrosive? how do you get the bleach on the paper towel?


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: ]
    #1716090 - 07/14/03 10:49 PM (13 years, 7 months ago)

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Anonymous

Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716101 - 07/14/03 10:51 PM (13 years, 7 months ago)

Lol ya they are pretty easy -_- ... sorry watched Dragon (the Bruce Lee movie) today.
BTW you didnt really answer my question do you use gloves or is it just a good idea?


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 2 months
Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716104 - 07/14/03 10:52 PM (13 years, 7 months ago)

Get gloves at Cosco or Sams or Price Club... 2 Boxes of 100 (that's 100 total pairs for the geniuses) for under $10. Or get small zip loc baggies in bulk, and use those as ghetto gloves.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: kukusz]
    #1716111 - 07/14/03 10:54 PM (13 years, 7 months ago)

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Offlinecharvo
JOURNEYMAN
Male

Registered: 03/09/03
Posts: 654
Loc: In The Hand Of The World
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Re: Innoculation and Sterilization HELP! [Re: LiL_KuSsH]
    #1716117 - 07/14/03 10:54 PM (13 years, 7 months ago)

if your at the point of knocking up your jars and your not exposing the grain to the air,what is the point in being so clean now all you have to do is shoot those jars up.am i missing something here.hygene is important in ones life but i think to be that clean just shoot up jars is just the wroug time to worry about it.

i would just make sure that needle is clean and blast your load in them jars good luck man.


--------------------


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Anonymous

Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716120 - 07/14/03 10:55 PM (13 years, 7 months ago)

Hmmmmmm... Ghetto glove huh... again the gloves are only for wiping down with bleach? or should I wear these "ghetto gloves" while im actually innoculating... Damn I could just picture that ugly picture right now :frown:


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 2 months
Re: Innoculation and Sterilization HELP! [Re: ]
    #1716141 - 07/14/03 10:59 PM (13 years, 7 months ago)

I wasn't reading the top of the post... my friend uses gloves (ghetto or not) when handling cakes in a terrarium, for harvesting, etc. When innoculating, he never uses anything, since he is only touching thing like syringes and jars. But this might not be considered the safest way.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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Anonymous

Re: Innoculation and Sterilization HELP! [Re: kukusz]
    #1716171 - 07/14/03 11:06 PM (13 years, 7 months ago)

So basically I should just wipe the work area down with bleach spray the room down with lysol give it a good minute to settle, light the syringe till red hot wipe it with bleach innoc. a hole , repeat?


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 2 months
Re: Innoculation and Sterilization HELP! [Re: ]
    #1716177 - 07/14/03 11:07 PM (13 years, 7 months ago)

I would not bother with wiping post flaming the syringe to red hot. You killed _everything_ flaming the needle, wiping it just reintroduces chance of contamination.


--------------------
All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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