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Offlinemerlink
IntergalacticTraveler

Registered: 04/30/03
Posts: 90
Last seen: 12 years, 5 months
Re-sterilize syringe between each quart jar?
    #1781337 - 08/05/03 01:02 PM (14 years, 15 days ago)

Should my buddy re-sterilize the syringe he is using after he innoculates a single quart jar? (innoculate --> sterilize --> innoculate --> sterilize --> etc.)

-Merlin


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"Once you have an experience, you experience it for the rest of your life ? as time is a man-made relativity, expiration of experience is a thought." - Merlin


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Re-sterilize syringe between each quart jar? [Re: merlink]
    #1781346 - 08/05/03 01:05 PM (14 years, 15 days ago)

My friend wipes the needle with an alcohol saturated pad between jars. Works for him.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Invisiblehoushroom
shroom cowboy

Registered: 05/07/03
Posts: 678
Loc: space city
Re: Re-sterilize syringe between each quart jar? [Re: merlink]
    #1781349 - 08/05/03 01:06 PM (14 years, 15 days ago)

it wouldn't hurt. instead of flame sterilizing every time try using just a lint-free cloth soaked in alcohol. that way you won't kill spores in the needle that haven't been exposed to air.


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Offlinenone
newbie
Registered: 08/02/03
Posts: 34
Last seen: 13 years, 11 months
Re: Re-sterilize syringe between each quart jar? [Re: houshroom]
    #1781730 - 08/05/03 03:05 PM (14 years, 15 days ago)

when you flame steralize the needle, everything that is in the needle at that time is killed including the spores.

Everything in the syringe that is not in the metal needle is left untouched. the metal needle is connected the syringe with a plastic fitting so flaming it doesnt harm the contents of the syringe.

I flame and then I dunk in alochol, give it a little dripity drip (let that alochol drip out or it will kill the spores).

always wait a few seconds before putting into jars after flame because dunking or wiping will cool it down but sometimes not enough,.


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Amazon Shop: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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