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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: I have tried everything and still cant get one pin and contamination [Re: PsyCode]
#5737221 - 06/11/06 04:45 AM (17 years, 7 months ago) |
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I saw a photo doc on the air movement of using the oven tek...
written by Anno. <-- my mentor!~
and it wasn't good...the air movement went right into the jar.
and the shroom and fishing gods...
call me the chosen one!~

tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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PsyCode
Stranger
Registered: 02/22/06
Posts: 36
Last seen: 14 years, 8 months
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Re: I have tried everything and still cant get one pin and contamination [Re: Roadkill]
#5737226 - 06/11/06 04:50 AM (17 years, 7 months ago) |
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that may be true, but my oven dishes out a high degree of heat that dissapates all particles from the work area, disinfected gloves and mask can't contaminate the air, thus the air, although clean I might add, are stopped by the dry verm anyway. I don't know what it is but I never get contamination...even on my very first try, that must be a SIGN!
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Roker
Stranger



Registered: 11/02/05
Posts: 343
Loc: outer spiral arm
Last seen: 7 years, 10 months
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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
#5737254 - 06/11/06 05:41 AM (17 years, 7 months ago) |
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Hi Gary,
I know where you're comming from, It took me three contaminated attempts before I found a method that worked for me (BTW i'm a bit of a lazy bastard and cleanliness is much closer to god than it is toO me!). I hope the following works as well for you as it does for me.
assuming you have a syringe ready to go?
I made up some mya agar in little plastic pots with small holes drilled in them. I taped these with sellotape then pc'd for 10 minutes.
flamed the needle then inoculated the cooled agar through the tape. then quickly taped over the hole - gave each container a shake and left it alone for a week or two.
then I went to Rush Wayne's website and bought his 1st pdf book. He is the originator of the H202 method of myclelial cultivation and worthy of support (in my opinion) so I won't give you proportions of h202 you can find all that from Dr Wayne or from reading up on the shroomery.
I made up some agar with peroxide and transferred some of the first lot of unperoxided agar onto it. It looks really crazy for a few days - the myc grows away from the peroxide agar into little islands until it releases enough enzymes to break the peroxide down and start to spread.
The great thing about working with peroxide is you don't have to worry so much about hygine. Once you've got your myc growing on peroxide agar then you can just give your workbench a quick wipe down, close the windows and get to it.
next step is make up some substrate (I like WBS). let it cool then add some h202 in some water and give it a good shake around(compensate for the added water when you pack your jars prior to PC'ing). Do this step quickly - no point in tempting fate.
Then slice up your agar and throw a few pieces into the jars - and leave them alone until colonised. Don't be tempted to shake them, I tried and got contamination, but only with the ones I shook. The others were fine, they just took a while to colonise. It probably took about four weeks to completly colonise my pint jars. I know that a lot of the growers here have full colonisation in 5 days but I was just gratefull to have no contams after a month that I didn't care!
It worked for me, I hope it works for you too.
Good luck
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garygenn69
Stranger

Registered: 01/25/06
Posts: 8
Last seen: 14 years, 7 months
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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
#5737288 - 06/11/06 06:55 AM (17 years, 7 months ago) |
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thnaks, you are right
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: I have tried everything and still cant get one pin and contamination [Re: garygenn69]
#5737341 - 06/11/06 09:17 AM (17 years, 7 months ago) |
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http://www.shroomery.org/forums/favlinke...r=1&postmarker=
I'm with Anno, Roadkill, and agar on this one. I really think you all should be too. They are 100% correct. The oven tek for inoculation is bogus. Your line of thought is flawed. sorry. You are getting lucky in my opinion. but whatever, just trying to help. I suggest you all ditch the oven method for real.
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