Liquid Mycellial Culture Question :-) - Mushroom Cultivation

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Mklangelo
enthusiast

Registered: 10/30/01
Posts: 297
Loc: Continental United States
Last seen: 22 years, 2 months

Liquid Mycellial Culture Question :-)

#529984 - 01/24/02 09:16 AM (22 years, 2 months ago)

Will a jar innoculated with a tissue culture liquid colonize faster than one done with simply a Mycellial Honey Water? Please assume of course that all other things are equal.

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[red] Life:[/red][blue] Live it foward, understand it backward...[/blue]

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Shdwstr
FSRCanada

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 3 months

Re: Liquid Mycellial Culture Question :-)

[Re: Mklangelo]
#530037 - 01/24/02 10:01 AM (22 years, 2 months ago)

A mycellium liquid culture will start to colonize a substrate immediately after inoculation. A liquid tissue culture is in a sterile solution to prevent mycellial growth, it will require an extra day for colonizing to begin. Depending on the strain, some liquid tissue cultures could start to form mycellium within hours.

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SouthernGent
veteran

Registered: 01/12/02
Posts: 1,331

Re: Liquid Mycellial Culture Question :-)

[Re: Shdwstr]
#530041 - 01/24/02 10:07 AM (22 years, 2 months ago)

really? can you buy liquid or is it legal?

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Shdwstr
FSRCanada

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 3 months

Re: Liquid Mycellial Culture Question :-)

[Re: SouthernGent]
#530063 - 01/24/02 10:27 AM (22 years, 2 months ago)

Sorry... you'll have to make your own liquid cultures.

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Mklangelo
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Registered: 10/30/01
Posts: 297
Loc: Continental United States
Last seen: 22 years, 2 months

Re: Liquid Mycellial Culture Question :-)

[Re: Shdwstr]
#530090 - 01/24/02 11:02 AM (22 years, 2 months ago)

I knew this dude that said it used to work like a charm with his Cambodians. Was he exagerating? Of course he was reffering to the Tissue Culture method.

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[red] Life:[/red][blue] Live it foward, understand it backward...[/blue]

Edited by Mklangelo (01/24/02 11:04 AM)

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Shdwstr
FSRCanada

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 3 months

Re: Liquid Mycellial Culture Question :-)

[Re: Mklangelo]
#530101 - 01/24/02 11:16 AM (22 years, 2 months ago)

He wasn't exagerating. Mycellium liquid cultures colonize quickly, overcoming contams. By using a tissue culture you get the added benifits of more uniform flushes and fruits due to the cloning.
You can also make a mycellium culture using your tissue culture.

Excellent results!

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ar393
old timer

Registered: 01/26/00
Posts: 702
Loc: VT

Re: Liquid Mycellial Culture Question :-)

[Re: Shdwstr]
#530133 - 01/24/02 12:08 PM (22 years, 2 months ago)

umm, once you have they honey water going, what do you think it is? its a liquid mycelium culture....#$#%$&^$
peace, ar393

Edited by ar393 (01/24/02 12:47 PM)

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Mklangelo
enthusiast

Registered: 10/30/01
Posts: 297
Loc: Continental United States
Last seen: 22 years, 2 months

Re: Liquid Mycellial Culture Question :-)

[Re: ar393]
#530247 - 01/24/02 02:34 PM (22 years, 2 months ago)

Yes, but not a Liquid TISSUE culture.

We were just differentiating between liquid cultures produced from spores and those from tissue.

:-)

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ar393
old timer

Registered: 01/26/00
Posts: 702
Loc: VT

Re: Liquid Mycellial Culture Question :-)

[Re: Mklangelo]
#530829 - 01/25/02 06:40 AM (22 years, 2 months ago)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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