best liquid/agar medium?

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gray1
addict

Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 23 years, 10 months

best liquid/agar medium?

#318497 - 05/16/01 10:16 AM (24 years, 9 months ago)

would those who are in the practice of liquid mycelial culture and agar mycelial culture please share their favortie media recipies?

is malt extract/water sufficient?

will fungi grow on LB, TB, and/or YPD?

c12h16n24ohdmt

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egghead
veteran

Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 23 years, 10 months

Re: best liquid/agar medium?

[Re: gray1]
#318511 - 05/16/01 11:01 AM (24 years, 9 months ago)

You could follow Adam Gottlieb's liquid agar tek. In that, you grow mycelium in this shakeable liquid agar suspension. To harvest, you just strain/filter it.

He gives these two agar formulas in the book.

Malt Extract Agar
20 grams malt extract
100 mg potassium phosphate dibasic (K2HPO4)
100 mg calcium carbonate
9.5 grams agar
1 L distilled water

Potato Dextrose Yeast Agar
250 grams of washed, unpeeled potatoes
10 grams dextrose
1.5 grams yeast
15 grams agar

Slice potatoes 1/8 inch thick
Rinse slices clean in cool tap water
Final rinse with distilled water
Cook in distilled water until tender
Strain and set aside cooking water
Rinse potatoes with distilled water
Retain rinse water, discard potatoes
Add distilled water to make 1 litre
Bring liquid to slow boil
Add dry ingredients

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