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OfflinePanTrop
enthusiast
Registered: 11/03/99
Posts: 288
Loc: Washington
Last seen: 20 years, 10 months
Re: bulk substrate/ Liquid mycelium
    #66978 - 11/29/99 11:06 AM (23 years, 15 days ago)

The Best source for info on the liquid mycelium culture teks is in Paul Stamets book "The Mushroom Cultivator" Liquid is definalty faster than jars, Im new and will be using agar as my next attemp but right now Im useing jars and wish I had just used the petri dishes and gone about the liquid path. About the Oven bag tek, dont waste your time with it, I tried it, and it went bad. Moe Howard a very respected moderator has tried it many many times with no sucess. Now it is possible to innoculate a Spawn Bag using the liquid Mycelium tek. Its too long to explain in a post but its in the book I mentioned earlier. But here is my bulk tek

Take a colonized MMGG/PF jar and crumble it to bb sized pieces add as much of the crumbled mycelium cake as you want into a spawn bag fill that spawn bag with sterile substrate seal, shake around to get the mycelium disributed and wait for colonization.....as for how long that will take. DOnt know this is my fist attempt at this bulk tek. I will post results when I have them in about 2-3 weeks though. to speed up your colonization of your jars try adding them to a container filled with water just enough so the water is about half way up the jars add a heating pad to the bottom of the container add towels to adjust the heat and that will help you want about 80-85F. Also if you havent, take the tape off! it only slows down the growth.

Hope that helped :smile:

------------------
"My mind dont work if my spine dont jerk"
-Quote from Eminem-

~PanTrop~



--------------------
" My mind dont work if my spine dont jerk!"


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Invisiblemycofile
Pooh-Bah
 User Gallery

Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: bulk substrate/ Liquid mycelium [Re: PanTrop]
    #66980 - 11/30/99 10:23 AM (23 years, 14 days ago)

If your new, and frustrated by failure, bulk is not the answer to your problems. Straight PF or MMGG is. The best way to speed colonization is heavy spore innoculation and proper incubation at around 82 degrees F.
Oven bags can be used succesfuly, but the official tek isn't the way to use them. Once you have some experience, improvise the tek a little.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Anonymous

Re: bulk substrate/ Liquid mycelium [Re: PanTrop]
    #66981 - 11/30/99 10:30 AM (23 years, 14 days ago)

Hey PanTrop, what about contaminization? How sterile was your environment where you added the mycelium to the substrate?

Please keep me informed. I hate doing so many jars and am messing with autoclave bags. So far I've melted one and made another bag of goop.

Am going to regress to brf/verm for the next attempt.



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OfflinePanTrop
enthusiast
Registered: 11/03/99
Posts: 288
Loc: Washington
Last seen: 20 years, 10 months
Re: bulk substrate/ Liquid mycelium [Re: PanTrop]
    #66982 - 11/30/99 11:37 AM (23 years, 14 days ago)

BuffaloTrance,

I have not actual done the Bulk tek I spoke of its my own version of a bulk tek....This will be my first time actually doing this....But, my sterile conditions will be the same as when I carried out my inoculations.....Work in a dentists office :smile: use an oven to help help keep contams away and the basice inoculation sterilzation will also be done.....with exposed mycelium and no Laminar flow hood I dont want to lose a bag or bags due to contams :smile: who knows if my version of a bulk tek works maybe it will replace Hips Bulk Tek :smile: Could Happen......

------------------
"My mind dont work if my spine dont jerk"
-Quote from Eminem-

~PanTrop~



--------------------
" My mind dont work if my spine dont jerk!"


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