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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Laminar Flow Hood, Petri Dish, Pressure Cooker

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OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 11 years, 2 months
Oven Tek for Inoculation
    #526192 - 01/20/02 03:12 AM (14 years, 10 months ago)

Does anyone here have experence with the Oven Tek where you use a oven set on low heat and use a pulled out grill for use as a flow hood? I have read much here via searching, it seems like a cheap reliable method for use in handling prints and syringies.

Just curious as to other's opinions.

Thanks


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>>Jammer>>


Edited by Jammer (01/20/02 03:25 AM)


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OfflineSThomas
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Registered: 10/16/01
Posts: 251
Loc: Ottawa, Ontario
Last seen: 14 years, 7 months
Re: Oven Tek for Inoculation [Re: Jammer]
    #526208 - 01/20/02 03:23 AM (14 years, 10 months ago)

Works like a charm, I use a little higher heat...but zero contams using this method so far. Cheers


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Offlinefresh357
addict

Registered: 09/02/01
Posts: 493
Last seen: 14 years, 7 months
Re: Oven Tek for Inoculation [Re: SThomas]
    #526214 - 01/20/02 03:32 AM (14 years, 10 months ago)

excellent idea...i'll never innoculate without it...it works too well. I use a little higher heat too...just be careful not to burn yourself.
stomas...cant you get a finer girl for your pic?


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OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 11 years, 2 months
Re: Oven Tek for Inoculation [Re: fresh357]
    #526223 - 01/20/02 03:45 AM (14 years, 10 months ago)

Im glad you two both brought up the subject of the heat. Some oven teks say to use the "lowest heat setting" while others say to use a "low heat setting".

Can anyone here advise as to how high the oven can be turned up before it's too hot to work around (granted, a stupid question) or how hot that it can be set untill the spores risk being killed.

(I live to ask stupid questions like this)

:)


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>>Jammer>>


Edited by Jammer (01/20/02 05:06 AM)


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Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/24/00
Posts: 5,015
Re: Oven Tek for Inoculation [Re: Jammer]
    #526283 - 01/20/02 04:56 AM (14 years, 10 months ago)

This method works excellent. CaptainMaxMushrooms, who is the orgin of the tek I believe, reports having a open jar on the rack of a oven set at *blah* tempurature for 20 or 30 minutes and no contamination. Also, I have made many syringes via. oven tek with no problem. Overall, a great low cost sterility tek.


--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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OfflineCheshirePhat
member
Registered: 12/13/01
Posts: 121
Last seen: 14 years, 4 months
Re: Oven Tek for Inoculation [Re: Jammer]
    #526387 - 01/20/02 07:47 AM (14 years, 10 months ago)

where can i get this tech??

does it work for BRF cakes??

is it somewhat like the PF tek but instead of a pressure cooker its an oven??

can i have a url to it please??

ta cheering


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[orange]"Lets fuck this kat"[/orange] -Elroy "tasmanian babes fiasco"


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OfflineNumba9
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Re: Oven Tek for Inoculation [Re: CheshirePhat]
    #526405 - 01/20/02 10:03 AM (14 years, 10 months ago)

I think you`re getting confused CheshirePhat. The oven tek is just used for innoculating your jars or agar transfers to petri dishes.... The hot air rising keeps air-born contams away from the work. The oven is not used in place of a pressure cooker for jars. I agree with the others... The oven tek works like a charm. i`ve done over 50 jars with no contams . I put my jars right inside the oven on a plate. Oven set low... about 150 or 140 no hotter... flame your needle in an alcohol flame and have a paper towel with lysol to cool the needle and further sterilize before entering the jars. Great tek... I`ll never use anyhing else now.



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Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


Edited by Numba9 (01/20/02 10:07 AM)


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OfflineSThomas
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Registered: 10/16/01
Posts: 251
Loc: Ottawa, Ontario
Last seen: 14 years, 7 months
Re: Oven Tek for Inoculation [Re: Numba9]
    #526512 - 01/20/02 01:34 PM (14 years, 10 months ago)

That girl is actually my fiancee...but turning the original into that size has distorted her a bit.


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OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 11 years, 2 months
Re: Oven Tek for Inoculation [Re: SThomas]
    #526989 - 01/21/02 01:32 AM (14 years, 10 months ago)

Want to hear something weird? When I first seen that pic under your handle I mentioned to my wife how some guys post pics of there girlfriends like you did. Right away she asked me "Thats probley not his girlfriend. How do you know it's his girlfriend"- and I didnt know how I knew.... And now I know that I asumed right...

really weird!


--------------------
>>Jammer>>


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OfflineSThomas
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Registered: 10/16/01
Posts: 251
Loc: Ottawa, Ontario
Last seen: 14 years, 7 months
Re: Oven Tek for Inoculation [Re: Jammer]
    #527152 - 01/21/02 08:47 AM (14 years, 10 months ago)

Yes that is my baby...she is also 7 months pregnant, with our first child...pretty excited about that. Cheers


--------------------
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OfflineCheshirePhat
member
Registered: 12/13/01
Posts: 121
Last seen: 14 years, 4 months
Re: Oven Tek for Inoculation [Re: SThomas]
    #527176 - 01/21/02 10:07 AM (14 years, 10 months ago)

heres an idea.......

when she goes oh tommy my water's broke.....

down about 20 grams and zoom to hospital.......

therefore shes great and if you fry your already there.....

it'd be fun

right?????????


--------------------
[orange]"Lets fuck this kat"[/orange] -Elroy "tasmanian babes fiasco"


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OfflineJammer
Computers areMORE Addictive!

Registered: 11/05/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 11 years, 2 months
Re: Oven Tek for Inoculation [Re: SThomas]
    #527837 - 01/22/02 02:18 AM (14 years, 10 months ago)

Congratts man!


--------------------
>>Jammer>>


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InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Oven Tek for Inoculation [Re: Jammer]
    #527862 - 01/22/02 03:07 AM (14 years, 10 months ago)

The highest heat setting that should be used can be found by increasing the temp untill you see the hair on your hands starting to burn, turn down the heat a tad from there and you've got it:).

Joshua 


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Offlinetomatofarmer
enthusiast
Registered: 01/10/02
Posts: 261
Last seen: 14 years, 9 months
Re: Oven Tek for Inoculation [Re: Joshua]
    #527966 - 01/22/02 07:42 AM (14 years, 10 months ago)

congrats my baby is 9 weeks old and she is a mess! but god was being very good whn he gave her us..we named her Dakota Ray Christian(we call her koti)
dude if u cultivate make sure the baby is out of it,they will take your kid for that u know...i dont cultivate but i got afoaf whop does..lol
peace
Lee


--------------------
I swear those mushrooms are going on my pizza officer!



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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Laminar Flow Hood, Petri Dish, Pressure Cooker

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