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Offlinegray1
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dmso & secondary metabolite biosynthesis
    #481848 - 12/06/01 12:59 PM (16 years, 13 days ago)

wonder if dmso has a similar effect in fungi.



J Antibiot (Tokyo) 2000 Oct;53(10):1145-53 Related Articles, Books, LinkOut

Enhanced production of microbial metabolites in the presence of dimethyl sulfoxide.
Chen G, Wang GY, Li X, Waters B, Davies J.
TerraGen Discovery Inc, Vancouver, BC, Canada.
Bacterial strains grown in the presence of low concentrations of dimethyl sulfoxide (DMSO) exhibit significant qualitative and quantitative alterations in the production of secondary metabolites. This effect was confirmed for a variety of biosynthetic families, including chloramphenicol (chorismate), thiostrepton (peptide) and tetracenomycin (polyketide), and for natural and recombinant strains of streptomycetes; a similar effect was seen with antibiotic-producing bacilli such as B. circulans. Increase in antibiotic production was not the result of a change in the growth rate of these organisms, since yields of biomass were similar in media with and without DMSO (up to 3%). We suggest that the addition of compounds such as DMSO provides a means of examining the full biosynthetic potential of microbes and might be used to promote secondary metabolite production. The mode of action of DMSO is not known, but in the cases studied it may act at the level of translation.
PMID: 11132960 [PubMed - indexed for MEDLINE]



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OfflineMAIA
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Re: dmso & secondary metabolite biosynthesis [Re: gray1]
    #481894 - 12/06/01 01:53 PM (16 years, 13 days ago)

"promote secondary metabolite"
Let me guess, "make more psilocyn and psilocybin".
If it could be applied to mushroom production in particular, this find could bring a new concept of "do it yourself genetical mutation" ... interesting.
Where can we find DMSO anyway ?

Peace,
MAIA


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Offlinegray1
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Re: dmso & secondary metabolite biosynthesis [Re: MAIA]
    #481908 - 12/06/01 02:11 PM (16 years, 13 days ago)

dmso is widely available in scientific labs and also through mail-order to the public
it's kind of a cure all pancea pharmaceutical but not recognized by the gov.
try dmso.org for more info.


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Offlinehoneyroasted
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Registered: 07/01/01
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Re: dmso & secondary metabolite biosynthesis [Re: gray1]
    #482058 - 12/06/01 04:28 PM (16 years, 13 days ago)

i'm thinking about ordering some of this - it's like 7 bucks for a pint. how much would you suggest adding on the first try?

anybody else have any thoughts?


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Offlinegray1
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Re: dmso & secondary metabolite biosynthesis [Re: honeyroasted]
    #482071 - 12/06/01 04:36 PM (16 years, 13 days ago)

i'll have to get ahold of that paper befoer i could even guestimate.
library-ho...........................


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Offlinehoneyroasted
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Re: dmso & secondary metabolite biosynthesis [Re: gray1]
    #482959 - 12/07/01 12:32 PM (16 years, 12 days ago)

bump


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