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Invisiblemrdasani
enthusiast
Registered: 10/19/01
Posts: 224
Very important question about change in Tek.
    #453711 - 11/10/01 06:23 AM (15 years, 1 month ago)

I already filled up my 12 jars and am waiting for the colonization of the mycelium. I wanted to produce more mycelium culture so I thought of a possible short cut to the whole process and would like to now instead of use jars, make the same ration ammount in oven bags to make a more bulk ammount. Is this a wise decision? I need to know if using bags of substrate would work. And if this would affect the rate of the colonization of mycelium....which I believe is highly posssible. More space means more time to colonize and more production of mycelium.

I was thinking of doing it like this:

Put the same substrate in a bag and sterilize it like usual, probably a bit longer. Then I would try colonizing the entire bag of subrate and use that as casing. Would this work or is the risk of contamination a very good possibility?

This really is just a way to avoid getting more jars...
Being extra care ful though, i see this as a possibility.

My main concern is this: IT TAKES 30 DAYS FOR A JAR TO FILL WITH A NETWORK OF MYCELIUM. I DON'T WANT TO HAVE ABOUT THREE FLUSHES FROM EACH CAKE AND THEN HAVE TO WAIT AN ADDITIONAL 30 DAYS TO START OVER, I WAS THINKING IF I OVERLAPP SOME PROCESSES, I COULD TOSS THE USED CASING AND PUT A FRESH NEW CASING IN THE PANS (OR CAKES). is this a good idea?


--------------------
"Come on you raver, you seer of visions,
come on you painter, you piper, you prisoner, and shine!" - Pink Floyd (Wish You Were Here 1975)

"Never underestimate the power of denial." -from American Beauty.


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Anonymous

Re: Very important question about change in Tek. [Re: mrdasani]
    #453713 - 11/10/01 06:26 AM (15 years, 1 month ago)

check out hippies bulk tek, but use a PC, not the oven, not boiling water... only a PC is gonna work for this one, also, consider a humidified colonization chamber... the bags can and will dry out.

also, on colonization time, jars seem faster, the bags I've seen start out slow.

get a print from the FSR, (took me 5 days after mailing the buck to ralphster, he's on top of his shit!)

get some syringes, go at it, be liberal with the spores on them bulk cakes.


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Invisiblemrdasani
enthusiast
Registered: 10/19/01
Posts: 224
Re: Very important question about change in Tek. [Re: Anonymous]
    #453716 - 11/10/01 06:39 AM (15 years, 1 month ago)

Also I'm wondering about the abhorts (the small dead pins that form on the top of the casing) Is this why some teks suggest the use of casing soil on top of the mycelium layer (To easily clean off the abhorts without losing the mycelium layer)? If I got this wrong, what is the Case Soil for then??? I am not too sure whats the purpose of casing soil above the substrate.


--------------------
"Come on you raver, you seer of visions,
come on you painter, you piper, you prisoner, and shine!" - Pink Floyd (Wish You Were Here 1975)

"Never underestimate the power of denial." -from American Beauty.


Edited by mrdasani (11/10/01 06:41 AM)


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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: Very important question about change in Tek. [Re: mrdasani]
    #453722 - 11/10/01 07:15 AM (15 years, 1 month ago)

Mainly to provide moisture.


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Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 14 years, 7 months
Re: Very important question about change in Tek. [Re: mrdasani]
    #454467 - 11/10/01 11:45 PM (15 years, 1 month ago)

You can prepare large amounts of substrate in oven bags and use small amounts of colonized substrate from a jar to inoculate the bags but your sterile technique has to be nearly perfect. Are your 12 jars 100% contam free? If so, you might think about working on a larger scale but usually large scale teks result in large scale disasters. The best bulk tek is spore print- to agar- to jars of grain- to pasteurized straw. You could mix one or more of your colonized jars with pasteurized straw and let the straw colonize to produce a larger batch of substrate. Pasteurized straw is much more resistant to contams than more of the same grain substrate so the step from grain to straw can be carried out with little attention to sterility.


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