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Amazon Shop: ½ Pint Jars, Agar, HEPA Filter, Laminar Flow Hood, Petri Dish, Pressure Cooker, Rye Grain

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Offlinefirsttimer101
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So you need a glovebox or flowhood to work with agar?
    #4045885 - 04/12/05 02:59 PM (12 years, 3 months ago)

I like working with agar because it's simple and hard to screw up but my recent experience has made it obvious that I can't open my jars to pop a piece of agar inside the jar. There must be so many spores floating around in this old house that even a few seconds of exposure is enough to contaminate the jars. Do most of you who work with agar use a flowhood or glove box?


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Invisibleeric_the_red
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Re: So you need a glovebox or flowhood to work with agar? [Re: firsttimer101]
    #4045915 - 04/12/05 03:03 PM (12 years, 3 months ago)

it's not worth working with agar without a flowhood or glove box.

the cost of contaminated jars/cultures/plates can quickly equal, then exceed the price of a box or hood. put that money towards something good.


--------------------


Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave


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Invisibleohmatic
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Re: So you need a glovebox or flowhood to work with agar? [Re: firsttimer101]
    #4045916 - 04/12/05 03:04 PM (12 years, 3 months ago)

no flowhoods/gloveboxes are optional if you are like me,
that is a total isopropanole junky with boxers on his hair,
gloves and facemask and working at the speed of light.

if you can manage this, check out drooldonkeys peroxide agar tek,
which led me to this, entirely without glovebox/flowhood:


and just to be fair, since noone's perfec:

peace ohm :mushroom2:

*edit*
the peroxide agar tek can be found here:
http://www.shroomery.org/index/par/23462

and dont mind eric's post up there, he says its not worth it but it can be
if you work super sterile and fast.
take my pictures as proove ..

*edit* #2
for love and peace :smile:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (04/12/05 04:00 PM)


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Invisibleeric_the_red
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Re: So you need a glovebox or flowhood to work with agar? [Re: ohmatic]
    #4045999 - 04/12/05 03:18 PM (12 years, 3 months ago)

Quote:

ohmatic said:
he simply does not have the experience
to tell as it seems




don't be presumtious. you know nothing of my experience. i have about 9 years of agar experience. i also have pictures of agar work years ago, without a flowhood. i never said it wasn't possible. i said it's not worth it.

Quote:


(please dont take this as an offence)




why should i take it any other way? you went out of your way to go back and edit a post to say that i lack experience and my words have no merit. so, please explain how this was meant to be taken, you wise individual.


edit:
maybe you think working without a flowhood or glovebox is the way to go. that doesn't mean the opposing opinion of the majority of those that work with agar is wrong.


--------------------


Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave


Edited by eric_the_red (04/12/05 04:03 PM)


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Invisibleohmatic
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Re: So you need a glovebox or flowhood to work with agar? [Re: eric_the_red]
    #4046195 - 04/12/05 03:59 PM (12 years, 3 months ago)

im sorry you are offended by my post as i seriously didnt mean to offend you.
please take a look at the picture of the dish showing aspergillus niger
to prove that i do get contams occassionally aswell.

btw, there is no need for you either to call me an idiot.

oh and actually i went to edit the post in order to include the link
to the drooldonkeys peroxide agar tek .. not for offending you.
to make you feel better, ill edit my post a 2nd time and remove the thingie
driving you so mad :smile:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleRoadkill
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Re: So you need a glovebox or flowhood to work with agar? [Re: firsttimer101]
    #4046286 - 04/12/05 04:13 PM (12 years, 3 months ago)

Yes you can work with agar without using a floowhood or a glovebox.

You would have to clean the living crap out of your work area though...and make sure that you don't have any drafts of air what so ever.

Your contam rate will be higher too.

I used to do agar work without using either...but I was extremely sterile in everything that I did.

I'd suggest building a cheap glove box...it will be a really good investment.

take care!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinegratefulredhead
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Re: So you need a glovebox or flowhood to work with agar? [Re: Roadkill]
    #4046364 - 04/12/05 04:37 PM (12 years, 3 months ago)

I've had little to no problems working with agar without either. However, I do use a hepa filter and work downstream from that.


--------------------


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InvisibleRoadkill
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Re: So you need a glovebox or flowhood to work with agar? [Re: gratefulredhead]
    #4046411 - 04/12/05 04:50 PM (12 years, 3 months ago)

Quote:

However, I do use a hepa filter and work downstream from that.




That would still be like using a flowhood...just a cheap one.

What are your contam rates like?


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinegratefulredhead
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Re: So you need a glovebox or flowhood to work with agar? [Re: Roadkill]
    #4046423 - 04/12/05 04:52 PM (12 years, 3 months ago)

True, Road. That is basically like a flow hood. Actually, I've never had any contams with agar (so far), and very few with the grain that I've transfered too. I'm having problems further down the line with casing, though... But that doesn't have much to do with this topic..


--------------------


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InvisibleRoadkill
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Re: So you need a glovebox or flowhood to work with agar? [Re: gratefulredhead]
    #4046444 - 04/12/05 04:55 PM (12 years, 3 months ago)

Quote:

I've never had any contams with agar (so far), and very few with the grain that I've transfered too.




That is great.

:thumbup:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinegratefulredhead
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Re: So you need a glovebox or flowhood to work with agar? [Re: ohmatic]
    #4046459 - 04/12/05 04:58 PM (12 years, 3 months ago)

ohm--nice pics. how'd you light those??


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Offlinefirsttimer101
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Re: So you need a glovebox or flowhood to work with agar? [Re: gratefulredhead]
    #4047751 - 04/12/05 10:39 PM (12 years, 3 months ago)

Thanks for the help guys..I've recovered from this morning's disappointment at finding all my jars contamed...gotta try again. I've spent too much money on pressure cooker, huge bags of rye, 15 or so petris, agar, HEPA filters, etc to give up on this.
I've probably spent about $700 CAD on this so far, which is quite a bit for me right now.

Now to construct a glove box..I hope this works!


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Offlinegratefulredhead
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Re: So you need a glovebox or flowhood to work with agar? [Re: firsttimer101]
    #4048649 - 04/13/05 02:52 AM (12 years, 3 months ago)

Go for it! This little endeavor is filled with ups and downs... Send us pics, should you succeed!


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OfflineMindzpore
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Re: So you need a glovebox or flowhood to work with agar? [Re: firsttimer101]
    #4049235 - 04/13/05 07:56 AM (12 years, 3 months ago)

Quote:

firsttimer101 said:
Do most of you who work with agar use a flowhood or glove box?



now to be honest i have to start by stating that i do not do agar work. but in regards to the glovebox... here is a little tips that should work for that as well. "the clean bag", its something i came up with long ago, when i was thinking of writing a " cheap-ass, lo-tech" TEK for cultivation. I've used it plenty of times (when i still used to grow) for inoculations, making monocultures or anything that needs clean environs. anyway here it is.

-The Clean Bag-

the only things you need are:
-a (large) see-through plastic bag,
-one (or two) rubber band(s),
-one plastic/latex glove,
-spraybottle with disinfectant (h2o2).
-a bottle of sterilized water (optional).

sterilize all the things you will be working with and put them into the plasticbag (if you lay the bag on a table, you can easily arrange the things on the table, inside the bag).

wash hand, dry, put on glove. make sure there is some air in the bag. put the rubber bands on your gloved arm, and put your hand at the mouth of the bag. use rubber band to secure the mouth of the bag around your wrist.

pull the bag and rubberband a little to the side and insert nozzle of spraybottle, and spray everything inside the bag to sterilize it. use your gloved hand to make sure it gets everywhere. remove spraybottle (and if your paranoid use second rubberband to secure/double seal the bag around your wrist.

wait 20 minutes (read a book).

by leaning the table and placing your wrist lower than the edge of the table, you can easily drain out the disinfectant (avoid getting it on your naked wrist).

if you are going to work with spores (or anything sensitive to your disinfectant), this is a good time to use the optional waterbottle to rinse items and insides of bag. drain as before.

now. you should have a relatively clean working space inside the bag. and do whatever procedures you need to. i find it easiest to work with the gloved hand and hold items through the plastic bag with the outside hand.

when your finished, close all jars/dishes and just open the bag.


it doesn't get much cheaper, easier, and stealth than this.

EDIT: suitable h2o2 concentration is around 6-7%.


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".


Edited by Mindzpore (04/13/05 08:34 AM)


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Offlinegoofy98
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Re: So you need a glovebox or flowhood to work with agar? [Re: Mindzpore]
    #4049356 - 04/13/05 08:45 AM (12 years, 3 months ago)

wouldnt that be 6-7% of 3% H202?


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Re: So you need a glovebox or flowhood to work with agar? [Re: goofy98]
    #4049359 - 04/13/05 08:47 AM (12 years, 3 months ago)

you want the total concentration of h2o2 within the agar not to exceed 1% of 0.3% h2o2.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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OfflineMindzpore
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Re: So you need a glovebox or flowhood to work with agar? [Re: ohmatic]
    #4053448 - 04/14/05 05:48 AM (12 years, 3 months ago)

yes, on or in stubstrate (like agar) you want lower ratios. but im not talking about actual agarwork, this is just about creating a sterile environ, forthe working.

but to sterilize the insides of the bag, outsides of jars etc. one needs higher conc. i of course assume that you have the sense to close all jars, sporesamples or whatever while spraying and transfering to/from bag.


--------------------
Mindzpores words of wisdom:
"If you think something is foolproof, you just haven't met proper fools".

Wiccan_Seeker said:
"It is better to adjust to become a better listener than to keep on cranking up the volume".


Edited by Mindzpore (04/14/05 05:51 AM)


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Amazon Shop: ½ Pint Jars, Agar, HEPA Filter, Laminar Flow Hood, Petri Dish, Pressure Cooker, Rye Grain

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