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Registered: 10/15/99
Posts: 1,396
Last seen: 5 months, 8 days
strain domestication
    #320644 - 05/19/01 02:32 AM (17 years, 4 months ago)

I was looking over an old Koh Samui post, and read that Ryche would run his wild prints through a few generations before distribution. Has anyone else noticed a change in behavior through 3-4 spore print generations?

As soon as the spores hit the agar the germinations and matings are selected that succeed on artificial media; doesn't this happen every time you inoculate anything with spores, regardless of whether it's 3rd generation or a wild print? Does pinning or fruiting really improve? Certainly recombination will change the makeup slightly each time, PF and B+ a couple of the long term results. How many generations did it take to come up with these strains? Many times many, I'd wager.

I had great success with an agar-birdseed-dung-multispore inoc. from a wild print, and cloned the nicest fruit; After reading Ryche's post, I'm wondering if it would be better to go through spore generations a couple times?? I've never played with anything but supposed 'domesticated' strains before. This wild guy was as fast as any on agar and birdseed, the more 'artificial' of my substrates.

The way I imagine the process, you would have more of the genes intact the closer you are to a wild outdoor specimen--representing recent selection for and against the variety mother nature has to offer. Could be wrong.

I'm sure Ryche or other vendors have their experiences, I'd like to hear;

Muchas grassy ass;

The above information is complete fiction.

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Registered: 07/25/00
Posts: 19
Last seen: 16 years, 9 months
Re: strain domestication [Re: Suntzu]
    #320787 - 05/19/01 11:27 AM (17 years, 4 months ago)

OK wild print multispore innoculation yeilded a nice fruit and you cloned it but the rest of the flush probably had something to be desired right? What you did is fine but what happens when you lose the culture? If you go back to the original wild print then you will get the same inconsistent fruiting. Take a print from one of the clones and multispore innoc, take a print from the nicest one and multispore again. The point of running it through a few generations is to get good, more consistant fruiting from your multispore innoculations. It should only take a few generations to accomplish as long as you only use prints from the nicest and healthiest fruits.

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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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