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Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Multispore vs. clone & woodlovers question
    #542303 - 02/06/02 09:13 AM (22 years, 2 months ago)

A followup question to all the talk of pure strain isolation methods and genetics:

What about woodlovers like P. cyan. and azurescens? Is it a waste of substrate to innoculate wood chips with a multispore culture, or will the fruiters win out like they tend to do with cubensis?

- AE

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InvisibleOlgualion
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Registered: 11/13/01
Posts: 1,253
Loc: Currently Earth...
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Re: Multispore vs. clone & woodlovers question [Re: altarego]
    #543784 - 02/07/02 02:00 PM (22 years, 2 months ago)

I am also wondering this...

And also, when isolating a strain on agar from multispore, how can one be sure he has a fruitable strain? In an ideal world, we could clone a fruitbody on agar to set up a bed. But if one wants to set up a bed with a single strain from multispore, it would suck if it can't fruit. Is there something to look for in the mycelium on agar such as rhizomorphism in cubensis?


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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 19 years, 18 days
Re: Multispore vs. clone & woodlovers question [Re: Olgualion]
    #544051 - 02/07/02 05:59 PM (22 years, 2 months ago)

I would say go multispore. Get as much variation as you can and then clone and collect prints from those that rise. I say this only because it is harder to test for fruiting ability with these guys as opposed to cubes. Ya know?


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InvisibleZen Peddler
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Registered: 06/18/01
Posts: 6,379
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Re: Multispore vs. clone & woodlovers question [Re: Olgualion]
    #545172 - 02/08/02 05:59 PM (22 years, 2 months ago)

forgive me if this is a stupid point, but i was wondering how one can isolate a good fruiting strain of Pan cyans - there arent really much in the way of rhizos to look for


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InvisibleNighted
Ghost

Registered: 11/09/01
Posts: 698
Loc: Funkytown
Re: Multispore vs. clone & woodlovers question [Re: altarego]
    #545958 - 02/09/02 04:37 PM (22 years, 2 months ago)

Ask yourself this question: Do I plan on taking prints to preserve my species for future cultivation?

I suggest you set up 2 or more patches, half using multispore cultures for future print making and the rest from isolates.



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Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: Multispore vs. clone & woodlovers question [Re: Nighted]
    #546034 - 02/09/02 06:22 PM (22 years, 2 months ago)

Seems there are no definitive answers here, so I'll be the guinea pig.

I have multiple agar plates with multi-strain azure and cyanescens cultures, which I will put on rye, then woodchips, then into beds.

I'll check back in the Fall with my results.

- AE

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