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Offlineamyloid
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Registered: 03/08/03
Posts: 980
Last seen: 7 years, 10 months
Re: Moved: Multi-Spore vs. Cloning [Re: ]
    #1476167 - 04/20/03 12:10 PM (18 years, 7 months ago)

yes, and yes, which is why many cultivators use 2 pure monokaryon strains, with good rhizomorphic traits, to innoculate spawn.


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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Offlineamyloid
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Re: Moved: Multi-Spore vs. Cloning [Re: BigJohnson]
    #1476170 - 04/20/03 12:11 PM (18 years, 7 months ago)

>>Again, do you think it would be possible to isolate a pan spore and a cube spore, introduce them to agar, and obtain a dikaryon from the colony? I doubt it, but I suppose it would depend on the number of chromosomes each have. Anyone know where this information can be found?

no, i fairly positive two differnt species wont "mate".


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Offlineamyloid
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Re: Moved: Multi-Spore vs. Cloning [Re: ]
    #1476187 - 04/20/03 12:18 PM (18 years, 7 months ago)

>>but may not fruit. only 1 in 4 pure strains of cubensis is capable of fruiting.

what is a pure strain? not a pure culture, considering no pure culture(a single monokaryon colony) would fruit.

>>an easier and faster way to get a pure fruiting culture is to clone a fruitbody in my opinion.

what tek are you using that fruits faster then spore+agar - pinning stage???


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Offlineamyloid
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Re: Moved: Multi-Spore vs. Cloning [Re: ]
    #1476195 - 04/20/03 12:23 PM (18 years, 7 months ago)

just as cells in your body can change genetically and become cancerous, growing myc can have variation in growth trends, making seperate cultures of rhizo myc. growth will definitly encourage more rhizo growth hence more pins.


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Offlineamyloid
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Re: Moved: Multi-Spore vs. Cloning [Re: poke smot!]
    #1476209 - 04/20/03 12:28 PM (18 years, 7 months ago)

maybe due to degreneration of the culture over time, also how many times have u seen that happen? i havent noticed a differnce in myc. growth with differnt spawn used to innoculate agar. also keep in mind that the starchy insides of rye grain encourages thick fluffy/cottonty (tomentose) type of myc.


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
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Re: Moved: Multi-Spore vs. Cloning [Re: BigJohnson]
    #1476314 - 04/20/03 01:08 PM (18 years, 7 months ago)

THE MUSHROOM CULTIVATOR will guide you and correct alot of misinformation you are hearing .


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
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Re: Moved: Multi-Spore vs. Cloning [Re: tripndicular]
    #1476551 - 04/20/03 03:41 PM (18 years, 7 months ago)

Okay, I have Stamet's book on the way :smile:

If anyone knows of a book that's a little more "cutting edge," please post it up. It doesn't even need to be about cultivating per say. I just want to learn more about fungidae; if dikaryotes exchange genes in a colony, the number of chromosomes typical for a haploid, etc.

If I had access to a microscope, I'd really enjoy germinating a pan spore and a cube spore on the same plate to see if the two form a dikaryote. I believe Anno said he had heard someone describing a multispore with the two producing very potent fruits, though that could be for reasons other than the two forming a dikaryote.


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Should the US relegalize drugs?

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Anonymous

Re: Moved: Multi-Spore vs. Cloning [Re: BigJohnson]
    #1479721 - 04/21/03 07:22 PM (18 years, 7 months ago)

A pure culture is one free of contamination, both visible and latent. Pure culture is also used to describe a culture made up of a single Dikaryon.

A fruit as a clone source is going to result in a culture exactly like the fruit it was taken from, less any random mutations LEADING to sectoring.

A colonized grain from within a multispore jar may have more then one Dikaryon present. When this is placed on agar, it can result in more then one Dikaryon visibly sectoring away from each other. The result can be more then one Dikaryon being isolated from the single grain. If the multiple Dikaryons are isolated away from eachother and replated. You can wind up with Multiple pure Cultures coming from a single transfer.

To avoid any False Dikaryons(non fruiting, or Sterile Dikaryons), it would be more advisable to grow out the multispore to fruits, and clone the best fruit with the ability to sporulate, if you are after spores, or non sporulating if spores are not a priority.

Anastamosis describes the interaction between two distinct Dikaryons when they come in contact. They can and do exchange (intact) haploid nuceli with eachother. (The same Strain will do so at a higher rate, then the interaction between different strains).

Without overcoming many barriers(structural, chemical, genetic, etc...) breeding a Pan with a Cubensis will be impossible. Simply placing a spore of each on a plate will not result in mating.

There are many books that might interest you, but the more specific your interest the better the books. General books, that incompass all aspects of Mycology tend to be extremely limited in the depth they go into each section. Understanding which area of mycology you are interested in will help narrow your search for a good book. Taxonomy, morphology, physiology, genetics, or cultivation.




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OfflineBigJohnson
Whoa! You guysneed to lightenup!

Registered: 02/04/03
Posts: 716
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Re: Moved: Multi-Spore vs. Cloning [Re: ]
    #1479862 - 04/21/03 08:10 PM (18 years, 7 months ago)

Thanks teo! That's what I've been looking for! Shrooms for you :smile:


--------------------
Should the US relegalize drugs?

http://www.lp.org/issues/relegalize.html


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