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OfflineDrOuD
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Registered: 06/15/04
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Last seen: 17 years, 1 month
Karo/Honey + PF + H2O2 (comments?)
    #3038991 - 08/23/04 11:42 AM (17 years, 10 months ago)

Seeing the standard Karo/Honey Tek, where you add H2O2 after germination to keep out contaminates, leads me to want to try a PF type cake with an H2O2 solution.

Normally the H2O2 would kill the spores. But we're not using spores, are we?

Has anyone tried this and had success?

The only problem that might occur is the break-down of H2O2 during pressure cooking. Does anyone know if this happens or how to avoid it? Would slightly dry cakes and an inoculation of H2O + H2O2 after sterilization work better?

If this isn't a duplicate idea, I hereby dub it the Nospore Tek and will write a nifty little guide.


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Invisiblemycopsycho
Tit Inspector.
Male
Folding@home Statistics
Registered: 06/17/04
Posts: 3,712
Loc: Going Nowhere Fast
Re: Karo/Honey + PF + H2O2 (comments?) [Re: DrOuD]
    #3039362 - 08/23/04 01:42 PM (17 years, 10 months ago)

i don't know about that, never tried or thought of it. but i can say that dunking in h2o2 is always good.


--------------------
I Am The Sickness.

Diploid: I think adults have a right to make stupid decisions and it's nobody else's fucking business.


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OfflineSpudz76
Got mycelium on the brain...
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Registered: 02/08/04
Posts: 391
Last seen: 12 years, 3 months
Re: Karo/Honey + PF + H2O2 (comments?) [Re: DrOuD]
    #3040407 - 08/23/04 07:08 PM (17 years, 10 months ago)

I have experimented with this on a few cakes which seemed to dry out before 100% colonization (incubator too hot). I made a mix of distilled water and H2O2 (wasn't measuring, but not a huge amount of H2O2), and injected the cake in four spots, so far it looks like it didn't so much absorb it like I had hoped, but I think they dried up too much before I tried to save them, and likely need to be dunked, ripped up, and cased or similar.

In general I swear by H2O2 when dealing with anything mycelium-culture related where spores are not involved. The option of leaving the water ratio low and then evaluatively adding liquids including H2O2 later when innoculating (with liquid mycellium) could work very well and certainly dispel most contamination problems both by making it easier to get the right water content (many times, either too much boils out or drips in depending on sterilization methods/errors) and also by using liquid mycelium which can take over a jar much faster (and thus be 'safe' on its own) well before a spore innoculation would. I personally have used spores on my first cakes but will likely never recede to spores again unless some mass infection makes me have to start over and none of my storage cultures work anymore, I like this to happen as fast as possible as do most people (it seems, in general - lots of "why no pins after 3 days WTF" and "no germ after 2 days what gives" posts).

EDIT: and, IMO, if you use enough H2O2 and live culture innoculations, you could skip the pressure cooker steps entirely. Yes I know this is probably a controversial statement, but I have done many none-too-careful setups (mostly EXPECTING them to contam) but so far nothing happened besides the intended stuff (mycellium, of intended type, growing well). So a substrate mix based on PF original, involving half of the water replaced by H2O2, and skip the PC steps, innoc with mycelium, done.


Edited by Spudz76 (08/23/04 07:17 PM)


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