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Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 3 months
H2O2 killed cakes :-(
    #1868066 - 08/31/03 12:53 PM (13 years, 3 months ago)

I just did a casing, and sprayed everything down with a <1% H2O2 solution and it completely did away with my cakes. No colonization whatsoever. When the solution hit the cakes, it turned bubbly-white, which i assume was the enzymes in the mycelium breaking down the h2o2. At first i thought my problem was that the peat moss was too acidic that i was using to case. Then i transplanted the colonized mycelium to a pure verm casing but the mycelium had already died.
The mycelium was definately alive when i cut it apart into whopper-sized pieces because a lil shroom popped out in the jar the day beforehand. The only thing that i couldve done to completely kill the mycelium IMO is spraying the H2O2. Its not like only parts of it werenet colonizing, there was absolutely no colonization. Im posting this as a warning, as everything ive read on the Shroomery has said that live, colonized mycelium can break down H2O2 very easily and that H2O2 will kill spores and contams, but NOT mycelium.
Anyone had any similar experiences with hydrogen peroxide?
~MC


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OfflineHaveABeer420
G.O.D.
Male

Registered: 04/29/03
Posts: 666
Loc: The Carnival of Souls
Last seen: 1 year, 29 days
Re: H2O2 killed cakes :-( [Re: mattch1]
    #1868070 - 08/31/03 12:54 PM (13 years, 3 months ago)

What ratio did you mix your H2O2 with water?  :smirk: 


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: H2O2 killed cakes :-( [Re: mattch1]
    #1868080 - 08/31/03 01:00 PM (13 years, 3 months ago)

My friend routinely dips colonized agar wedges in 3% h2o2 for a few seconds before transfering to other plates or jars. It slows mycelial growth temporarily but they always recover.

As an experiment he once misted a pinning casing with straight 3% h202 to see what would happen. The pins all stopped growing and aborted a couple of days later.


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Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 3 months
Re: H2O2 killed cakes :-( [Re: dog]
    #1868105 - 08/31/03 01:09 PM (13 years, 3 months ago)

i bought it as a 3% solution. Then i diluted it about 5-10 h2o:1 H2o2. pretty weak IMO.


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Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.


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OfflineHaveABeer420
G.O.D.
Male

Registered: 04/29/03
Posts: 666
Loc: The Carnival of Souls
Last seen: 1 year, 29 days
Re: H2O2 killed cakes :-( [Re: mattch1]
    #1868147 - 08/31/03 01:21 PM (13 years, 3 months ago)

10:1 is what my pit bulls have always been told.  They were also told to be pretty exact about it, if your solution was in fact 5:1 then you are probably right about it killing the myc.  I wish you better luck in your future mycological endeavours, atleast you'll never make that mistake again.  :smirk: 


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I don't charge by the inch I charge by the foot, think I'm lying bitch here take a look...100% U.S.D.A grade A beef, heres my card call me.


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Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 3 months
Re: H2O2 killed cakes :-( [Re: HaveABeer420]
    #1868930 - 08/31/03 07:16 PM (13 years, 3 months ago)

This was a diluted solution of an already diluted 3%!! solution thou....


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Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.


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Anonymous

Re: H2O2 killed cakes :-( [Re: mattch1]
    #1868944 - 08/31/03 07:22 PM (13 years, 3 months ago)

your supposed to dilute 3% 10:1...you had waaay too much h202...you shouldn't see bubbling when you spray your cakes


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 6 years, 11 months
Re: H2O2 killed cakes :-( [Re: ]
    #1868998 - 08/31/03 07:49 PM (13 years, 3 months ago)

When green meanies show up on my friend's casings he sprays them with like 4:1 water to 3% solution H2O2, it bubbles and fizzes, and the casing does fine... so different shit happens to everyone...


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 3 months
Re: H2O2 killed cakes :-( [Re: kukusz]
    #1869271 - 08/31/03 09:56 PM (13 years, 3 months ago)

Of course something else couldve happened to the mycelium. The only thing I did that affected ALL of the mycelium was cut it up into whopper sized pieces, spray it with h2o2 water, put on h2o2 sprayed potting mix, and put h2o2 sprayed potting mix on top. After that I incubated at around 85 degrees for 5 days. I saw absolutely no growth, so, thinking the problem was with the pH of the potting mix. I took out all the pieces of mycelium and put them in the middle of a vermiculite sandwich. Thats it. Nothing abnormal except for the transfer. Again, I saw absolutely no growth after 5 days. When I recased, I took one piece of mycelium and put it in between 2 pieces of karo soaked cardboard, and, again, NO growth.... so, the only thing i can think is the h2o2.......
~MC :-(


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Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 6 years, 11 months
Re: H2O2 killed cakes :-( [Re: mattch1]
    #1869900 - 09/01/03 01:56 AM (13 years, 3 months ago)

My friend has had issues like these before - make sure you don't have light cobweb forming in there, it sounds more like contam then killing ALL of the mycelium.


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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Offlineluigi
journeyman
Registered: 07/15/03
Posts: 71
Last seen: 7 years, 4 months
Re: H2O2 killed cakes :-( [Re: kukusz]
    #1870546 - 09/01/03 07:22 AM (13 years, 3 months ago)

I always use h2o2 almost 1/2 and 1/2 and never had a problem.What strain was it?It sounds like contam to me.


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Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 3 months
Re: H2O2 killed cakes :-( [Re: luigi]
    #1870799 - 09/01/03 11:57 AM (13 years, 3 months ago)

even with contams, the mycelium would have at least recuperated a little bit. I also doused everythign with h2o2, which supposedly kills contam spores.... contams are a possibility, but if it was, it completely killed ALL of the 1/2 pint of mycelium before it could even recuperate from cutting... Also, i used VERY sterile technique... but contams are always a possibility... :-(
~MC


--------------------
Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.


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