Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour

Jump to first unread post. Pages: 1
Offlineacidhead1279
Master Of AllThings Hip
 User Gallery

Registered: 03/01/03
Posts: 532
Loc: somewhere on the other si...
Last seen: 11 days, 1 hour
karo and cloning
    #2299218 - 02/04/04 01:26 AM (13 years, 1 month ago)

so i want to make an isolate from the best fruit. i know how to prepare the karo and have made liquid inoculants from spores, but i dont know how to take a sterile culture from the fruit body. can anyone send me in the right direction?


--------------------
Farewell FRSE!  We had such good times together.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineGratefulDread
Never pass up anopportunity topee.

Registered: 10/21/02
Posts: 366
Last seen: 12 years, 6 months
Re: karo and cloning [Re: acidhead1279]
    #2299283 - 02/04/04 01:53 AM (13 years, 1 month ago)

Go Here and instead of using the BRF paste use the syringe to innoculate your karo jar.


--------------------
Before you criticize someone, you should walk a mile in their shoes.
That way, when you criticize them, you're a mile away and you have their shoes.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineacidhead1279
Master Of AllThings Hip
 User Gallery

Registered: 03/01/03
Posts: 532
Loc: somewhere on the other si...
Last seen: 11 days, 1 hour
Re: karo and cloning [Re: GratefulDread]
    #2299304 - 02/04/04 02:02 AM (13 years, 1 month ago)

um i think i asked the wrong question... is it possible to say take a tissue culture from the stem of a fruit body, dip it in h2o2, shake it off, and then drop in some sterilized karo? on the karo thread DeanOfMean posted a pic of something along these lines but doesnt really elaborate.


--------------------
Farewell FRSE!  We had such good times together.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineGratefulDread
Never pass up anopportunity topee.

Registered: 10/21/02
Posts: 366
Last seen: 12 years, 6 months
Re: karo and cloning [Re: acidhead1279]
    #2299344 - 02/04/04 02:18 AM (13 years, 1 month ago)

you would be using the tissue from the stem of a fruitbody. just the grinding then soaking in H2O2 would minimize the contaminant likelyhood. You could also use any tek for cloning with agar but with any of those you would have to open the lid of the karo jar and expose it to contaminants. by grinding the fruitbody up, soaking it in H2O2, putting it in a syringe and injecting your karo jar with it you are lessening your karo's exposure to contams.


--------------------
Before you criticize someone, you should walk a mile in their shoes.
That way, when you criticize them, you're a mile away and you have their shoes.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* stems into h202 soak for karo clone ADoobie 895 7 02/01/05 01:18 AM
by ADoobie
* when cloning
( 1 2 all )
forevrgrounded98 1,801 26 11/06/03 03:14 AM
by Rose
* differences betwen strain isolation by transfers and by cloning?
( 1 2 3 4 all )
FGL 9,454 62 10/06/09 10:13 PM
by feelfunny
* Cloning with out agar, ??? Sulli 876 18 08/16/08 05:47 PM
by Peterthinks
* Cloning from dried Caps!
( 1 2 all )
kiddd038 2,741 32 10/09/08 09:21 PM
by djmako7
* ghetto cloning tek? Techn9cian 360 3 05/07/09 09:37 PM
by Techn9cian
* Explain to me the joys of substrain isolation (karo jars etc) IfIWereARichMan 1,398 14 03/24/10 06:09 PM
by rberg79
* Honey Water Cloning GratefulDread 2,353 3 12/03/09 07:49 PM
by Kush44

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
545 topic views. 35 members, 128 guests and 32 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Sporeworks
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.016 seconds spending 0 seconds on 14 queries.