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Offlineacidhead1279
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karo and cloning
    #2299218 - 02/04/04 01:26 AM (13 years, 10 months ago)

so i want to make an isolate from the best fruit. i know how to prepare the karo and have made liquid inoculants from spores, but i dont know how to take a sterile culture from the fruit body. can anyone send me in the right direction?


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OfflineGratefulDread
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Re: karo and cloning [Re: acidhead1279]
    #2299283 - 02/04/04 01:53 AM (13 years, 10 months ago)

Go Here and instead of using the BRF paste use the syringe to innoculate your karo jar.


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Offlineacidhead1279
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Re: karo and cloning [Re: GratefulDread]
    #2299304 - 02/04/04 02:02 AM (13 years, 10 months ago)

um i think i asked the wrong question... is it possible to say take a tissue culture from the stem of a fruit body, dip it in h2o2, shake it off, and then drop in some sterilized karo? on the karo thread DeanOfMean posted a pic of something along these lines but doesnt really elaborate.


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OfflineGratefulDread
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Re: karo and cloning [Re: acidhead1279]
    #2299344 - 02/04/04 02:18 AM (13 years, 10 months ago)

you would be using the tissue from the stem of a fruitbody. just the grinding then soaking in H2O2 would minimize the contaminant likelyhood. You could also use any tek for cloning with agar but with any of those you would have to open the lid of the karo jar and expose it to contaminants. by grinding the fruitbody up, soaking it in H2O2, putting it in a syringe and injecting your karo jar with it you are lessening your karo's exposure to contams.


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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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