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First timer: growing too quick to be target mycelium? * 1
    #29722339 -

Hi,

Apologies in advance for the noob post.

First time grower here. I inoculated 2 bags and 2 agar plates 4 days ago 5/11.

Both were supposedly spore syringes but 1 appeared to have mycelium strands which I was chuffed about from my prior reading. I inoculated the bag and put a drop on the agar, however after I sealed the plate I dropped the plate and the droplets covered the lid… haha I know, dumb ass. But had a little look around 50+ hours of inoculation and it looks like that plate has one little very fine spider web structure looking like thing developing on it. 2 nights passed, checked again 9/11 and it’s growing at a pretty quick rate. Is it too soon?




Second syringe was clear with specks, so assuming it was legit spores in liquid. Inoculated bag and plate. But I notice there is growth on that plate too, but suspiciously there are two tiny little circles that are smaller than the other plate. Is that more significant of contamination since I only did one drop? First pic is 50+ hours and second pic is after an additional 2 nights. The centre looks dark in the pics, but there is so much condensation on the lid and so it’s hard to get a good view, but shining flashlight through side, all the centres currently look white. On the bag of this one, there is the tiniest amount of white growing around a couple pieces of grain. Most people would probably miss it when viewing.




I tried to make everything sterile but I did not have a SAB. I tried to jimmy one up in a very dodge manner, turning a small table on its side after sanitising, cling wrapping around it leaving a small gap at the bottom and sanitising again. I closed all windows, vacuumed, wiped everything down, left it for an hour to let the air settle and then I closed the door and blocked the under door crack to try minimise air flow. Then I sanitised everything with alcohol. So I did try my best, but admittedly I am super new and likely imperfect. It was also pretty windy outside and hindsight I probably should have waited for a day that it wasn’t. Plus my dodgy SAB was so small, I had to keep taking my hands in and out of it. Probably worth noting that it is pretty hot here at the moment at 27 degrees Celsius… I know everything likes to grow in this humid heat.

Thoughts from experienced growers - is this likely contamination?

I’m guessing it’s just going to be a time will tell thing. I have ordered a SAB that is arriving today and will put another couple drops on agar to see how they grow.

Thanks for taking the time to read and response. :smile:


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Re: First timer: growing too quick to be target mycelium? [Re: CeeGood] * 1
    #29722393 -

It's certainly not too soon and yes you're correct it is a time will tell


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Re: First timer: growing too quick to be target mycelium? [Re: cronicr] * 1
    #29722409 -

I am a little more optimistic about the specimen that looked like liquid culture in the syringe, but everything I read about spores is that it takes weeks for germination - is that not correct? And the specimen that has several small patches, I only put one drop of liquid on the agar, yet growth in several places. I think I inoculated bag first and then agar, which looking back was dumb.

I wasn’t expecting to see anything for weeks and I’m unsure whether to be excited or disappointed. 😂

Posting grow bags as I might make this a little journal of progress.

Day 5 - grow bag inoculated with what I suspect was liquid culture




Day 5 - grow bag inoculated with spore syringe




--------------------
See no evil, hear no evil 🙏

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Re: First timer: growing too quick to be target mycelium? [Re: CeeGood] * 1
    #29722413 -

Fresh spores can germinate in a day or 2 , older spores may take longer


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Re: First timer: growing too quick to be target mycelium? [Re: CeeGood]
    #29722705 -

As cronicr mentioned, fresh spores can germinate on agar in a few days while older spores can take 1 - 2 weeks to germinate.

I noticed the grains in your grow bags have a bacterial problem. This might be an issue later on in your cultivation. If you see bags of grain with slimy yellow-ish liquid on the grains, its likely bacterial and it is best to not use that type of grain as bacteria is a form of contaimination.

The mycelium on some of your plates looks really transparent. I would wait more spores germinate before doing any transfers. I find the best mycelium is the pure white type.

And that syringe with the floating mycelium could also be liquid inoculate (LI), which is mycelium suspended in clean water. This is one of my favorite ways to inoculate substrate. If its clean, it works great! Very fast colonization.


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Re: First timer: growing too quick to be target mycelium? [Re: LogicaL Chaos]
    #29722715 -

Thanks for the info folks!

@LogicaL - I’m not seeing any yellow liquid or yellow in either of my bags. Are you seeing that or what specifically says bacterial issue in my bags? Do you see it in both??? 😭

Yes, growth looks thin, but I assumed because it’s only early? Will it thicken with time?

Also, no one sort of mentioned whether it’s normal to have several colonies. I feel like that might be a sign that it’s contam? With the one plate being dropped, it makes sense that there is an additional colony growing to the side, but the one plate with several spots seems suspicious since I only placed one drop… maybe 2.

I bought the grow bags (all in one) from a large online retailer… should I not and just attempt my own grain? Was just trying to make it as simple as possible for my first time.

Also, I purchased pre-poured agar plates, but looking at getting stuff to make my own… what’s better - glass petri or just buying bulk disposable plastic?


--------------------
See no evil, hear no evil 🙏

Little lady in the southern hemisphere :whiteflag:

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Re: First timer: growing too quick to be target mycelium? [Re: CeeGood]
    #29723879 -

Day 7 update

Culture 1: Plate growth looks very thin but little hair like structure forming towards the outside. Someone suggested perhaps mouldy sample. Concerned about grow bag as the white at the bottom of the bag appeared 2 days ago, but no more advancement. Wondering if it’s because I should have used the entire 10ml syringe during inoculation, as I’m pretty sure there was mycelium in the syringe. Going to try and be more patient with this one, but if I don’t see any progress in a week, should I inject more LC or perhaps sterile water?





Culture 2: I think looks more promising despite being more concerned initially. Plate has lots of growth that is all white, but I’m still concerned about grow bag.




I inoculated 2 more agar dishes in my new SAB 2 days ago. Starting to see tiny growth on plate. Feeling a bit more confident in these ones as I did everything I could to prevent contam. Looking forward to watching how these ones develop in comparison to my first two. Will wait until I have a good sample before I inoculate anymore grain.




--------------------
See no evil, hear no evil 🙏

Little lady in the southern hemisphere :whiteflag:

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Re: First timer: growing too quick to be target mycelium? [Re: CeeGood]
    #29723922 -

First culture looks way off to me. The powdery dot look in the grow bag isn't a good sign, either.

Second one looks like what you want, but I echo Logic's concern about bacteria. A close-up would help more, but wet-looking grains partly pressed against the bag is one of the telltales. For the plate, take transfers from the leading edge of the growth if you haven't already.


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Re: First timer: growing too quick to be target mycelium? [Re: MethodMyst]
    #29723955 -

Bummer about the PE plate and bag!

I’m suspicious of the GT bag… maybe the side of the bag looks how I expected, but the bottom looks weird and maybe I’m seeing a hint of yellow? Close ups of that bag.




Is it not too soon to do a transfer? They are all so small, I’m not even sure which colony I would take a culture from to transfer?

I still have about 5ml in my PE syringe, thinking about trying to expand the LC since the bag and plate aren’t looking good?


--------------------
See no evil, hear no evil 🙏

Little lady in the southern hemisphere :whiteflag:

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Re: First timer: growing too quick to be target mycelium? [Re: CeeGood]
    #29724978 -

You should try to germinate the PE LC on another plate, and certainly don't try to expand it. What you have on the first plate is mold. However, it may have entered due to a hole in you're sterile technique.

Keep making transfers from that second plate: move a small piece from the leading edge of the mycelium in attempt to clean it up as much as possible.:mushroom2:

Spores are inherently dirty, and shouldn't be sent to grain. LC can be dirty as well and should be tested on agar before sending it to grain, for good measure. The benefit of agar being we can see the contaminates and isolate the cleaner sections accordingly

Keep us posted

:camping:

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Re: First timer: growing too quick to be target mycelium? [Re: Agar Wedge]
    #29725145 -

So I did do another PE LC on another plate and it grew exactly the same. I literally bleached the crap out of everything, I even sprayed bleach water in the air and ran two air purifiers in the room for hours prior. Then I bleached the counter again and let it sit, then I 70% alcohol and then set up SAB. I 70% the inside twice with the air purifiers off, 70% wiped everything I put into the SAB and then I sprayed everything with 70% in the box again and just let it sit closed up for an hour. And when I went to work on it, sprayed my gloves etc and was so careful. So the fact that the same growth occurred from both the GT and the PE (which were from different suppliers) has me thinking either that the contam is in the syringes or I am growing what I am supposed to.

Sample 1 PE 5/11





Sample 2 PE 9/11




Agar slice transferred from sample 1 PE on 11/11, photo taken around 24 hours later



Sample 1 GT 5/11





Sample 2 GT 9/11





Agar slice transferred from sample 1 GT on 11/11 (noticed next day that slice was on the lid instead of agar so I shook the plate until the slice landed on the agar. Growth is roughly 12 hours after relocation.



Some photos make the cultures look dark in the centre, but it’s just because I’m photographing from behind the agar due to condensation. But everything is white so far. I’m still cautiously (and maybe ignorantly) optimistic… 🤞


--------------------
See no evil, hear no evil 🙏

Little lady in the southern hemisphere :whiteflag:

Edited by CeeGood (11/12/26 07:40 AM)

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Re: First timer: growing too quick to be target mycelium? [Re: CeeGood]
    #29725179 -

Lookin pretty good !

Thanks for joining us.  Appreciate you posting.


Time for some transfers. Just grab what looks nice and lets to to t1!


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