Doozberry said:

You think those are for sure knots on the sides there?  Idk why, but they look more flat/squished than regular primordia/knots to me.

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Fiet said:

guys, if you ic a species ore 3 which one you would order from that list?

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Rasmodius said:

I'm gonna UTFSE, but first, I'm gonna not:

What's everybody's tek like for getting spore print sheets? Do people cut dedicated ones and PC a bunch at a time or somethin? I usually just unroll direct from the thing, assuming it's sterile-ish (a-la-wedge-bags) on the inside of the roll from the factory, and put a shoebox over it. But for prints where I actually wanna try hard, seems like surely something better can be done.

Now off to search....

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Doozberry said:

Hey first time poster but long time lurker here. The quick question I have is in regards to a frustrating streak of shoebox failures I've been having.  Specifically involving whatever is going on in these boxes. It has  been throwing me off because it looks sort of similar to knot formation.  I really haven't been able to find any other pictures of this contam/bacteria/mutation whatever it is?  I know 2 weeks is a little early for APE pins, so I'm just gonna let these ride for at least another 2 weeks.  I've had multiple shoeboxes stall at this exact stage without showing much knotting or any pinning at all and its driving me a little nuts!

Here is the surface of one of the SB's and the side of the SB with the strange bumpy stuff.  This one has exposed grains on top (like I usually run).



and this second one is the same aside from it being dubtubbed like in Mushboys SB tech which I wanted to try out as an unrelated experiment.  Only other difference is the light top layer of coir which was added at spawn.



Other info:
Strain : APE
innocced pressure cooked corn with LC syringe from vendor on 10/08
s2b on 10/27 using field capacity coir, 1:1 ratio.
standard 6qt sb
temp: 69-71f
lights: 12/12 6000k
misting: havent needed to yet.
Smell is good, surface conditions are good ( I think anyway), overall stuff appears to be fairly similar to many of the other successful APE shoebox grows I've done. 
I only open em once a week for pictures.


In any case, I've gotten over my irrational anxiety and started my agar journey.
I can accept that this is probably due to bacterial issues because of using a lc syringe directly to grain.  It's just confusing to me as I've had great results with this exact method for years, though I get it can be a crap shoot. 


I would still love to learn or understand this problem in a little more depth beyond just "LC to grain = bad". (as I said, I've begun agar) Could this be environmental?  Are these boxes fine?! Am I crazy?!



Lastly

thank you to all the TC's on this site.  I wouldn't be where I am today if it weren't for all the free information on here. 

thank you in advance to anyone who can shed some light on this.

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Welcome to the shroomery Doozberry!

Appreciate you signing on man. I had a culture once that did this constantly, all the jars, all the tubs, couldn't get anything past what you are showing. Indeed frustrating. I think you are correct in regards to it being a compromised LC. I couldn't clean that culture up for the life of me and ended up getting a new print and moving on without the same issue.

Great job starting you're agar journey. Gonna work out really nice for you

Thanks for sharing!

Agar Wedge said:

Doozberry said:

Hey first time poster but long time lurker here. The quick question I have is in regards to a frustrating streak of shoebox failures I've been having.  Specifically involving whatever is going on in these boxes. It has  been throwing me off because it looks sort of similar to knot formation.  I really haven't been able to find any other pictures of this contam/bacteria/mutation whatever it is?  I know 2 weeks is a little early for APE pins, so I'm just gonna let these ride for at least another 2 weeks.  I've had multiple shoeboxes stall at this exact stage without showing much knotting or any pinning at all and its driving me a little nuts!

Here is the surface of one of the SB's and the side of the SB with the strange bumpy stuff.  This one has exposed grains on top (like I usually run).



and this second one is the same aside from it being dubtubbed like in Mushboys SB tech which I wanted to try out as an unrelated experiment.  Only other difference is the light top layer of coir which was added at spawn.



Other info:
Strain : APE
innocced pressure cooked corn with LC syringe from vendor on 10/08
s2b on 10/27 using field capacity coir, 1:1 ratio.
standard 6qt sb
temp: 69-71f
lights: 12/12 6000k
misting: havent needed to yet.
Smell is good, surface conditions are good ( I think anyway), overall stuff appears to be fairly similar to many of the other successful APE shoebox grows I've done. 
I only open em once a week for pictures.


In any case, I've gotten over my irrational anxiety and started my agar journey.
I can accept that this is probably due to bacterial issues because of using a lc syringe directly to grain.  It's just confusing to me as I've had great results with this exact method for years, though I get it can be a crap shoot. 


I would still love to learn or understand this problem in a little more depth beyond just "LC to grain = bad". (as I said, I've begun agar) Could this be environmental?  Are these boxes fine?! Am I crazy?!



Lastly

thank you to all the TC's on this site.  I wouldn't be where I am today if it weren't for all the free information on here. 

thank you in advance to anyone who can shed some light on this.

---

Welcome to the shroomery Doozberry!

Appreciate you signing on man. I had a culture once that did this constantly, all the jars, all the tubs, couldn't get anything past what you are showing. Indeed frustrating. I think you are correct in regards to it being a compromised LC. I couldn't clean that culture up for the life of me and ended up getting a new print and moving on without the same issue.

Great job starting you're agar journey. Gonna work out really nice for you:)

Thanks for sharing!

---

Ah so you think they're no good?
thats what Ive been worried about.  Its been 2 or 3 spore syringes from a vendor, to be clear, not my own LC. Other times their stuff has been great but maybe they had a bad batch or 2?

Otherwise, Ive had to "dial in" a new area recently and I thought it was something I was doing wrong, but Ive been following well established EZAF teks of Bod's for awhile now and  I'd like to think theres not really much to dial in with these un-modded Shoe-boxes and some good ol field capacity coir.

I started agar 7 days ago with one of their APE syringes, heres how it looks now.  You think its worth trying to transfer any of these 3 plates or or should I just throw these out and grab a few more syringes from a different vendor maybe?

I got the pix from the underside, as there is some condensation on the top of the dishes. (Been keeping em upside down)  Please ignore my horrible saran wrap job! I got some parafilm now, lol.

CloudNyne420 said:

So i was wanting to start growing sum bears head n sum different oyster verities but i need hard wood substrate for those does any1 know what would be best for these n any brands tht work better then others?

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CloudNyne420 said:

So i was wanting to start growing sum bears head n sum different oyster verities but i need hard wood substrate for those does any1 know what would be best for these n any brands tht work better then others?

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I have gone through a few brands, some claim to be hardwood and clearly softwood (mainly) with a little hardwood in there, but I use Competition blend from Pittboss primarily, but any of the HWFP from Pitt work fine, Hickory, Oak, whatever, but the Comp bag is usually on sale more than the others.  40lbs for 15 bucks these days, I can make 20 ish blocks depending on if I make up a few bigger ones (7.5lbs is at capacity).  I am a tent grower, I couldn't speak on trying them in tubs or some other type of fruiting chamber.  I have left blocks to colonize too long and have the fruits start growing through the 2T filter, even creep through the ziptie at the top of the bag at times.  One of my FAVS to grow. 






<edit>  I thought I uploaded this pic... invitro style

Fiet said:

guys, if you ic a species ore 3 which one you would order from that list?

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CloudNyne420 said:

Awesome thanx for the info also did u have to add any wheat bran or soy hulls for xtra nitrogen i was reading it said tht could help boost yields jus wondering if r what exactly u used??

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