Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com APE Liquid Culture For Sale

Jump to first unread post Pages: 1
Liquid cultures from isos
    #29641237 -

I received some Albino Penis Envy Isolate that I tried using to make liquid culture.

I am unaware if my previous successes were from isolate or not but they dont seem to be germinating this time. Is there a reason for that? Also. Ive tried seeking answers elsewhere but it is still unclear to me.

Thank you for any insight. Its been a couple weeks now with no activity. If itd be better to just put it to grain Id like to get on the path of least resistance.

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: gaianempress]
    #29641314 -

germinating where, in the LC or in agar? Is it an LC mix you've used before? What sugar source?

LC to grain isn't without some risk, but for quality vendors that risk is quite low, if you're willing to gamble some lost time and a few cents worth of grain, fire away.

depending on your location this time of year, they may have gotten very, very hot in a mail truck or something too. quite a few heatwave casualties across the pond lately.


--------------------
.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.
:salute: CAKE WARS III STARTS SEPTEMBER 1st :salute:

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: gaianempress] * 1
    #29641316 -

I wouldn't go to LC's if you're looking for the path of least resistance. You're adding more frustration that is unneeded.

What I would do though is get set up to do agar to check your vendor bought items before putting anything to grain.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: Rasmodius]
    #29641548 -

Thank you for your responses. So..I get spores syringes from a vendor and then usually add 1-11/2 ccs to a 200 ml jar of sterilized water and light malt extract to make liquid cultures to get more from my syringe. Have had great success in the past with this..I thought about the heat during transfer..:/

So I could take isolates and essentially do the same as the multispores as long as they are still viable?

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: gaianempress]
    #29641569 -

Spore syringe to LC isn't a great plan. Also "isolate" is marketing fluff, but it looks like they already got your money...


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: KlarnEvil9]
    #29641588 -

It might not be a "great plan " but its def worked for me almost everytime Ive done it effectively turning 1cc of a 10cc syringe into 20 other 10cc syringes.. im ballin on a budget amigo, lol. I appreciate you giving some.insight tho. I def feel the marketing fluff and yea, they most certainly got me..

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: gaianempress] * 2
    #29641595 -

Russian roulette can similarly seem like a "great plan because it worked out for me" 5 times...

If you're "ballin on a budget" why the fuck are you giving $ to vendors who are trying their best to fuck you out of said $???

If you hang out in this community long enough, you will gain access to the marketplace. And the knowledge required to make use of all that entails. Reddit ain't got shit on that, nor do vendors.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: gaianempress]
    #29641601 -

To address your original post - if you had success doing this, then you bought vendor LC. Not a spore syringe. That shit doesn't work. Well, maybe 0.00000001% of the time it is possible, so you better get to work to make that non-zero percentage come true.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: KlarnEvil9]
    #29641952 -

I dont know what im missing.. Ive only gotten multispore syringes, eith purply spores that I use to inoculate a light malt extract & water solution. Wait about a week and have hundreds of angelic floating bodies that I have inoculated grains with and had a plethora of successful yields for years except for this isolate..

I use vendors because I haven't leveled my game up to spore printing and being self sustainable..yet.

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: gaianempress] * 1
    #29642067 -

If you're new to this hobby, You're not ready for LC yet.


--------------------
~ I used to get high on life, until I realized life was cut with morons ~
* You need 2 wake up and smell the music! *
- We are all computer data in a materialistic world -
| Sometimes you have to lose yourself, to find anything |

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: Blazer420] * 1
    #29642523 -

Ive literally used spores syringes to make LCs and have had many yields from them..what do u mean? Also..be cool if people would stay on original topic instead of telling me what I am and am not ready for. I came here for insight.."im not ready" isnt good enough. Thx anyway, tho

Extras: Filter Print Post Top
Re: Liquid cultures from isos [Re: gaianempress]
    #29642540 -

Maybe your isolate cooked in the mail? Do you have supplies for testing the isolate on agar or BRF cakes to test for viability? Pictures of the syringe, without vendor info, and your LC would be helpful. Include your method for preparing LC.
You grow using methods that aren’t recommended here  and the responses are based on that. You’re looking for insight and members are looking for info so they can troubleshoot and be helpful.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com APE Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Liquid Culture Questions - Clear LC's vs Dark, Cloudy LC's (more complex nutrients) bruiser9811 143 4 08/04/26 03:45 AM
by bruiser9811
* Easy grow kits vs. Liquid culture. Shroomhead2 110 2 08/05/26 02:06 PM
by cjbinks
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )		 agar 56,605 43 07/08/23 07:04 AM
by Screwup
* Liquid Culture Challenges Leimana 10,071 4 05/31/05 11:33 PM
by Corporal Kielbasa
* 1st Liquid Culture, arrrgh! Seril 15,572 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid Culture Recipes MrMaddHatter 25,531 13 03/29/05 04:53 AM
by Sbealzebub
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 6,823 12 05/08/05 03:55 PM
by lordoftheshroomz
* Clear liquid cultures ? mickywilliams2 3,974 11 10/12/05 05:02 PM
by Bamaman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
154 topic views. 17 members, 569 guests and 54 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.008 seconds on 16 queries.