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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Orange growth on the glass outside an almost fully colonized PF cake — metabolite or contam?
    #29640538 -

Hey everyone,

This is my first attempt at growing mushrooms, so I’m still learning how to read the mycelium.

I’m using a PF Tek-style setup with 360 cc wide-mouth jars, a BRF/vermiculite substrate, and a dry verm layer. The jars were inoculated with a GT liquid culture syringe and have been colonizing for roughly four to five weeks. With a temperature between 18 and 22 °C.

Most of the jars look fine. The jar in the pictures is almost fully colonized and should be ready for birth after a few more days of consolidation.

The strange thing is this orange growth on the inside of the glass, above the top of the cake. It appears to be completely separate from the substrate and I don’t see it growing through the cake. It first appeared a two weeks ago and grew a little at first, but it has remained basically unchanged since then.

There is also a thin halo of white mycelium around the orange spot, almost as if the mycelium has grown over or around it.

I have noticed some white mycelium growing on the glass in other jars as well, probably from a small amount of nutrient residue left on the glass during inoculation. So I’m wondering if this orange spot could be growing on a similar patch of residue, or if it is actually a bacterial or fungal contam.

The dark specks visible in the photos look like vermiculite particles to me. They are not spreading, and I don’t see any obvious green or black mold in the cake.

My questions are:

1. Does this look more like a metabolite/exudate, mycelium growing on leftover nutrient residue, or an actual contam?
2. Has anyone seen mushrooms produce orange or yellow metabolites on the glass like this?
3. If the cake itself looks clean, would you birth it carefully and keep it separate, or would you consider the jar too risky?
4. When I eventually remove the cake, should I simply leave the orange spot alone and remove the cake without letting it touch the glass, or is there any better way to handle it?

I only have one fruiting chamber, so I’d rather not risk my other clean cakes for the sake of one questionable jar.

Thanks in advance for taking the time to read this and for any advice you can share. I really appreciate the help from this community.

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Re: Orange growth on the glass outside an almost fully colonized PF cake — metabolite or contam? [Re: SMDJ] * 1
    #29641322 -

ooooh that's a little unhappy, whatever it is. the white halo makes it look like it might be a pocket of myc that's dumping a shitload of metabolites, but that would mean something is stressing it out. and being near the rim like that, maybe something it's fighting off.

The darker spots on the left side of the pic are also looking a little wet / undercolonized, and there's a good amount of condensation in there.

You're right that it's not worth risking the other grows.

If you did want to try it in a quarantined area just to see, I'd open the jar carefully and with a gloved hand and an damp alcohol paper towel try to just get underneath and bleach that entire spot off the glass, and scrub it clean a bit. Probably do the whole inside rim and lip for good measure. Let the alc evap off so it doesn't stress the rest of the myc needlessly. Clearing the road before the rest of the cake goes over it, basically.


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.~`  𝔅 𝔞 𝔤 𝔢 𝔩  🥯  𝔅 𝔦 𝔱 𝔢 𝔰  `~.


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Re: Orange growth on the glass outside an almost fully colonized PF cake — metabolite or contam? [Re: Rasmodius]
    #29641355 -

I've had stuff show up like that in grain jars with bacterial issues, usually with older/weaker cultures.

There's definitely some bacteria causing stress in your jar. The things Rasmodius listed above are the signs. I've had decent flushes from bacterial spawn, but they are way more prone to turning green too. If I had only have only one fruiting chamber, I'd leave that one out.

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Re: Orange growth on the glass outside an almost fully colonized PF cake — metabolite or contam? [Re: hummingbird]
    #29641872 -

Thanks a lot for the detailed replies, both of you. I’m going to read up on the “high bacterial load” issue, since I have a few other jars that don’t show any visible contamination, but do have some damp-looking areas and loose vermiculite around the edges. I had assumed the loose vermiculite was simply caused by not mixing it thoroughly enough with the BRF, but now I’m wondering if it could be related to excess moisture or bacterial issues. I’ll keep a close eye on those jars and definitely keep this one out of the fruiting chamber so I don’t risk the other grows. I really appreciate the advice.

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Re: Orange growth on the glass outside an almost fully colonized PF cake — metabolite or contam? [Re: SMDJ] * 1
    #29641886 -

I’d like to see the other jars. How did you prepare them? If all the jars have shoulders then how are you going to birth them?

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Re: Orange growth on the glass outside an almost fully colonized PF cake — metabolite or contam? [Re: The Tao]
    #29642226 -

Hey, thanks for asking!

I prepared these using an adapted PF Tek method, based mainly on this guide:

https://www.shroomery.org/forums/showflat.php/Number/24179086

I couldn’t find suitable wide-mouth jars without shoulders, so the best option I found has an 84 mm body diameter and an 82 mm opening. My plan is to let the cakes fully colonize and consolidate, hoping they shrink enough to pass through the opening when I birth them.

If they don’t come out cleanly, my contingency plan is to break them up and use them as spawn for a small bulk substrate rather than forcing them out of the jars.

All of the jars were inoculated on the same day, but they are progressing at very different rates. I plan to wait until each cake has completed about one week of consolidation, and then move each one to fruiting individually when it is ready, rather than putting all of them into fruiting conditions at the same time.

Because temperatures are relatively low here, I’ve been using the air conditioner to help stabilize the room, but the jars have mostly been colonizing between 18 and 21°C.

I also considered importing proper shoulderless jars, but the shipping cost alone would have been roughly $80 because of their weight, which made them far too expensive. These jars were much cheaper and seemed worth trying.

I’ll attach pictures of the other jars as well. They don’t show any obvious contamination, but some have damp-looking areas and loose vermiculite around the edges. One theory I have, although this is just a beginner’s guess, is that as the colonized cake shrinks, some of the vermiculite from the top layer falls into the gap that forms between the cake and the glass. I’m not sure whether that makes sense, though.

I initially assumed the loose vermiculite was simply caused by not mixing it thoroughly enough with the brown rice flour, but after the feedback about possible bacterial load, I’m taking a closer look at them.

If you have successfully birthed cakes from this type of jar, I’d be very interested to hear how you handled it. If you need pictures from different angles of any particular jar, just let me know and I’ll be happy to take them. Thanks for taking an interest and for taking the time to help me figure this out.








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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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