AstroSporeFiend said:

jee3apha said:

AstroSporeFiend said:

SAB work is based on the principle of still air, not sterility.

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Hey, I never said this. I mean, I wholeheartedly agree with what Mr. Piggy said there, but I can’t take credit for someone else’s comments.

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Mr Piggy said:

jee3apha said:

Yep, I know, but paper towels can be (and are) dirty. I put plates caps there during operations, caps that will then be moved over sterile agar, hence I think it can't hurt to have it soaked with isopropyl.

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Just caught this, this is where contamination is coming from.  DO NOT set the lid face down on anything, and alcohol is not sterile.  Alcohol can in fact introduce contamination to your work.  You should only be lifting the lid off the plate momentarily to inoculate or transfer, do not set it down.  If you do have to set it down, absolutely never place it face down on anything.

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Dave Bennett said:

If you don't like losing dick swinging contests don't challenge people to dick swinging contests.

Show me a brand new Tek for an "impossible" to cultivate species that you developed.

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There he is, there's the prick under the polite veneer. 

See Dave, the thing is, lots of people were growing them before you.  You seem incapable of acknowledging this even though you constantly post in an indoor semi cultivation thread that you did not start.  Who started it, why were there so many pages of posts before you showed up?  Why are there already documented grows of semis indoors on this very website?  Doesn't sound all that impossible to me

You're not the first, you're not special, and you don't actually know that much about cultivation.

You're just an AI addict with pretty steep AI psychosis that has been convinced by a fucking program that he's the smartest guy alive

jee3apha said:

Mr Piggy said:

And again, it would really help if we could see your SAB setup.  Kitchen counter is a perfectly fine place to do work, it's where I started.

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Will take a picture tomorrow! Nothing fancy, it's an IKEA SAMLA 65 L with 2 holes.

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Here it is:



The black spot near the right hole is there because I tried to solder a crack. The fire is actually my stove, outside the picture. The light on top is turned off because if fucks with my camera, but it's super bright, that was quite an improvement.

Dave Bennett said:

When I searched the forum I couldn't find a single post from anyone who got Liberty Caps to fruit indoors.

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Kloakk said:

Dave Bennett said:

When I searched the forum I couldn't find a single post from anyone who got Liberty Caps to fruit indoors.

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That is because most of the indoor semis grows is only found in journals. Sorry for being a lazy cunt and not providing links, but they are there. That said, yours is the most successful one that I've found, I believe the key component in your grows was limited resources.

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ReverendMyc said:

Here is a post on page 8 of the semi thread that shows several indoor grows dating back as far as 20 years. Many were not super good flushes, but it is difficult to call it not "a single post from anyone who got Liberty Caps to fruit indoors". This is the thread that Dave's 4th - 24th posts are in. He had a cool accomplishment, but his first post on page 30 of the thread while claiming to be the first when this comes from the same thread on page 8, takes some of the credibility away from his research and reading comprehension abilities.

I am not saying that his arguments are not reasonable. My contention all along has been that evidence based claims are more valuable than research based claims because you can limit the variables to the actual application rather than similar but unequal conditions. However, in several cases in various threads when members point out errors or flaws in some of the reasoning to Dave, he doesn't not address the specific critiques, not of himself but of the examples presented, and instead switches tactics. That has been the cause of much of the animosity from my observations. Not everyone is willing to take the time to spell all of this out. Some people are far more succinct than I, though possibly less diplomatic in their discourse.

I agree that being able to grow one species or another of mushroom does not qualify a person to address the complexity of organic chemistry. But I do not agree with spouting anything as fact without compelling evidence. Synthesis of and extrapolation from similar but unequal studies will never be compelling evidence in the face of experimental results that contradict them. That is the starting point of discovery, not the conclusion.

What the experienced users who are getting their feathers ruffled seem to be taking offense to are the many claims given without hard evidence that contradicts all of their accumulated experience. Not to say that there may not be ways to improve, but claiming one method is better than another without direct evidence is going to rub people the wrong way. What those experienced users share with new growers are methods that have been proven simple and successful over many years of accumulated practices. We do make changes in recommended best practices over time with sufficient proof of improvement. But claims without direct evidence will be met with reasonable and healthy skepticism as they should be. That is no different in the scientific community than it is on this forum.

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Oh for sure, I was clear with Dave, his approach is counter productive, because it causes more confusion than clarity or help, it was ignored, which is fine, but I moved on.

I do believe that Dave has a place here though, he just needs to, like Rasmodius said, learn to read the room. Most of the new members don't need the nitty gritty, like nietzche said: "What good is the molecular structure of water when one is drowning".

The molecular structure of water is interesting for some of us, but not while we're drowning. That said, besides the fact that Dave keeps focusing on one test without the whole picture I like his posts. I believe he is right for the most part.

Does heat degrade potency? Most likely, but so what? So does oxygen and water, the thing about Dave is that he makes a statement without context, and that will create confusion for those that don't want to get down to the root of the thing. I have tried to take it up between the lines of my post, but here we are.

At least piggy got Dave to react like a human, so that means he's not a machine, thank Ra. Aaaaaaaaanyway I'm looking forward to when you have the time to do more test with heat drying

I'm just here to learn and share what little I know

blakeyface said:

[...] Finally I went to AI, described the whole scenario and Ai says that panaeolus bisporus is illegal to grow and it won't conspire to cultivate illegal mushrooms. Then I went to another Ai, described the whole scenario but said that my spore print was pan agaricus. Ai confirmed that what I had was some version of 'filamentous fungi' probably some version of mold and that, yes, mold could cross trenches and burrow through cabins just as easily as mushroom mycelium. So, was there nothing that I could do to clean it up? It said that the one strategy that works for spore prints riddled with mold spores is by altering the PH of the agar, that I should use calcium hydroxide to raise the PH of my agar to 8.5 or 9.0, which greatly inhibits mold. Long story short: the popcorn fully colonized and 5 trays are now colonizing. I really hope this works...hope, too that I find other prints of bisporus. If any of my story is off, I make no pretense of expertise

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blakeyface said:

one more thing. years ago, when I used to grow cubensis, I never did a casing. Then I began doing pans, which, from what I'm told, benefit from a casing. Most advice has been to pasteurize the casing and to make it alkaline so as to inhibit mold. When AI instructed me to make the agar with a high PH, it felt like an aha moment...high PH doesn't hurt the mushroom mycelium but it stunts mold. So are you suggesting that a higher PH to casing is bunk too?
Like I said, this was a suggestion from AI, not me, I am not a theoretical mycologist...no dog in this race

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