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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: Dave Bennett] * 1
    #29597640 -

Dave Bennett said:
Apologies! I completely missed that the blue plates were water agar. You can scratch what I said about a potential fungal contam unless it's growing way faster than cube mycelium normally does (like filling the plate in a matter of three or four days). Cube mycelium grows thin and wispy like that on water agar, especially if the transfer is small which limits the available nutrients for any growth. So it looks like it's good news all round.

Some agar brands seem to be more prone to showing halos but if you wanted to enhance their visibility on agar add some soluble starch, like potato or corn starch, to your agar recipe. Making the agar slightly softer helps the enzymes diffuse out more efficiently too. Exactly the same process is happening when mycelium consumes the dye or colourant in agar plates too.




Hogwash.  Seriously.  Almost everything you say is
Hogwash.

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: BeefExtreme]
    #29598361 -

Can you elaborate? Saying something someone else says is hogwash then not correcting them is not productive. Everything they've said has made sense so far, please elaborate. It would save me a great deal of time :smile:


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Knowledge above all none sense.

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: No-Pilot-555] * 1
    #29598385 -

No-Pilot-555 said:
Can you elaborate? Saying something someone else says is hogwash then not correcting them is not productive. Everything they've said has made sense so far, please elaborate. It would save me a great deal of time :smile:



We've got a new issue emerging in this era of academic interest in psilocybes coincideing with the rise of AI.

People are turning up with mountains of very convincing-sounding text, and it's never quite clear how much of it is even coming from their own experience and outlook versus just whatever AI told them the tl;dr from a bunch of articles was.

And it creates a real conundrum for a lot of us. Arguing with this slop point-by-point just fuels the fire. When you're talking to someone who can't see the forest for the trees, arguing over individual trees isn't very productive. And saying that someone who sounds nice and helpful is spewing out hot garbage makes us sound like dicks. Especially because chatgpt NEVER sounds like a dick.

What Beef just quoted there is just a whole lot of wildly overconfident statements about how things work when making agar. I mean, literally every single statementt there is either something that I haven't found to be true in my own experience with agar work, some half-assed theory that probably came from hallucinating AI, or both.

Oh, and just for the record, no, you can't always see bacteria or even tell that they're in the mix. I mean, actually, they're basically always in the mix. They are literally the oldest and smallest form of life in existence.


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ACAB  🖕🧊


Edited by B Traven (06/07/26 12:26 PM)

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: No-Pilot-555]
    #29598397 -

You're welcome! It sounds like we're very similar in our love of mycology and the desire to understand as much as possible about the organisms we're fascinated by and working with. I grow purely for pleasure too and as I can grow way more than I'm going to take in a year in one grow the majority of my work is experimental. There's still so much to discover about the Psilocybes and huge gaps in the research which means that even amateur mycologists and citizen scientists can make discoveries that are new to science and make meaningful contributions to the scientific knowledge of the genus.

Going back to your question, yes, if the thin ring of mycelium growing out from the transfer in the blue water agar isn't growing really fast and more like a normal cube mycelium growth rate it's likely to be cube mycelium. Try taking a transfer of the leading edge and put it on nutrient agar and see what it does. Try bog standard MEA agar. 2g agar/2g LME, 100ml water as some of the abnormal growth you're seeing on the other plates could be due to excessive nutrients. A agar sugar content over 2 to 4% (depending on species) is known to inhibit and cause abnormal growth.

It was a pleasure chatting you you too!


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Edited by Dave Bennett (06/07/26 12:48 PM)

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: No-Pilot-555] * 3
    #29598472 -

No-Pilot-555 said:
Can you elaborate? Saying something someone else says is hogwash then not correcting them is not productive. Everything they've said has made sense so far, please elaborate. It would save me a great deal of time :smile:



B pretty much summed it up but yeah man, bacteria isnt always visible and theres been a lot of great developments to separating bacteria and healthy myc . Cabin sequestering etc. i apologize for not clarifying.

Anyhow, had i been around from the get-go on your thread i would have started by saying:

Hi! Welcome to the shroomery and thank you for being with us.

Your cultures really look pretty good for the most part but some of that growth pattern seems a little fishy.  Could have a sneaky bactera in there so what i would recommend is just doing a few transfers of the ropey looking myc you have onto a standard 2% agar recipe. (2% lme/ 2% agar) do this until you have a culture that you can feel 100% good about. 

All the rest of the speculative stuff is whats hogwash.

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Ignore this my phone does not like the mobile version of this site, sorry! [Re: No-Pilot-555] * 1
    #29599528 -

This was an error ignore.


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Knowledge above all none sense.

Edited by No-Pilot-555 (06/08/26 09:26 PM)

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: B Traven] * 1
    #29599557 -

That's extremely extremely unfortunate that AI is so rampant that it's even up on the shroomery just spewing nonsense. Wow that's upsetting. I actually thought I had a pretty good idea on what to do and where to go from this point.

Thank you greatly for elaborating. I usually double check and confirm things that I find when researching but I was being a little too trusting. I'll keep my eyes out from now on. Just a shame we even have to.

Either way thank you for going about it the way you did and explaining it to me before I took of regurgitating the trash.

Based off my OP though, I was just trying to get as much info as I could and find out if my plates looked sus or not. With the spiky mic and the weird thin/translucent myc, I was worried. Especially since they looked like they came from health cultures.

I understand that about the bacteria and thank you for further explaining it. I use microscopy usually to make sure and test things when it comes to my samples and cultures. Of course you always see bacteria I just wanted to make sure I didn't have some sort of infestation going on with the spiky mic and the weird thin/translucent myc or either of those being something I should worry about.

Thank you though, I will keep my eyes out for this slop in my future endeavors :smile:


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Knowledge above all none sense.

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: BeefExtreme] * 1
    #29599571 -

No no it's all good. If you'd have said they were just using some sort of AI I'd have been completely understanding. Yesterday morning I was going over this post and happened to look at their picture and realized it was AI and started getting sus myself. Some a disgusting thing to see in a field like this too. I don't want to get into that whole thing though, thank you regardless.

I have tested cabin sequestering (currently I am at least.) and looks promising so far. Thank you for the welcome! :smile: sounds good to me. Was mostly referring to the spiky mic and the weird thin/translucent myc. Trial and error! Just exciting times trying to revive some old genes. I'm loving it. Just want to get to the bottom of some of these pics for future reference.

Thanks again, on the heads up. I'll be a bit more speculative and check people's pages out and such. I'm going to completely discount what they said in this thread.


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Knowledge above all none sense.

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: No-Pilot-555] * 1
    #29599577 -

I mean, dave isnt totally wrong always, he just has a way of making things more complex than needed.

Just transfer till you get a pretty culture, thats it!

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: BeefExtreme]
    #29599579 -

Okay good to know, thank you :smile:


--------------------
Knowledge above all none sense.

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: Dave Bennett] * 2
    #29599951 -

Dave Bennett said:
I grow purely for pleasure too and as I can grow way more than than I'm going to take in a year in one grow the majority of my work is experimental.




Funny with all the bad advice we have seen you give you have yet to post pictures of a single grow.

Edited by RockinRobot (06/09/26 09:02 AM)

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Re: Believe to have bacterial plates and not sure what happened please help! Old gene library revival [Re: RockinRobot]
    #29599957 -

RockinRobot said:
Funny with all the bad advice we have seen you give you have yet to post pictures of a single grow.



Plenty over in the semilanceata thread. And even though Dave might now give the best advice because not everyone wants to get down to the molecular level of understanding how to grow mushrooms and that his answers can come across as confusing because he gives an in depth answer, I would say most of them isn't wrong, at worst they are counterproductive because the person on the other end don't understand them.



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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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