Home | Community | Message Board


Azarius
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Petri Dish

Jump to first unread post. Pages: 1
OfflineBD_Man
Stranger
Registered: 01/28/02
Posts: 5
Loc: IN
Last seen: 14 years, 9 months
Black Dots Under Mycelium(sp) in petri plates
    #534402 - 01/28/02 10:33 PM (14 years, 10 months ago)

Under the mycellium there's several little black dots they're about the size of lead for a mechanical pencil (.07). you can't see them unless you look from the bottom of the plate, or cut it open as i did when i made some new petri plates (with care to avoid the dots). any help would be great as it what they are and cause. Thanks


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSuntzu
Geek
 User Gallery

Registered: 10/15/99
Posts: 1,394
Last seen: 1 month, 4 days
Re: Black Dots Under Mycelium(sp) in petri plates [Re: BD_Man]
    #534520 - 01/29/02 01:09 AM (14 years, 10 months ago)

Are you growing on peroxide or antibiotic media?

Sometimes bacterial contaminants won't prosper in a treated petri dish, but find a nice little nook under growing mycelium. Worst case scenario, but still a possibility, is that it is an anaerobic/capnophilic bacterium that can ONLY thrive in the low O2 environment under the growing mycelium. This would also be consistent with the slow growth rate of the suspect contaminant.
No matter the case, you are 'probably' ok transferring mycelium as long as you stay away from the black points. 'Probably', meaning that the relative size and growth rate of fungal hyphae might allow a few bacterial cells to hitch a ride to the outer growth areas. If it is really an anaerobe, though, this is quite unlikely. If the antibiotic does its job, this is also quite unlikely.
Another likely scenario is that it is just a black precipitate, though such things are usually much smaller than a pencil lead.
If you have some more plates, it might be a good idea to attempt a tiny transfer of periphery tissue [perhaps while making some test inoculum].

**just read, you are doing transfers. . .I bet they'll be fine, take extra care to make it a small amount.


Edited by Suntzu (01/29/02 01:11 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 30 days, 10 hours
Re: Black Dots Under Mycelium(sp) in petri plates [Re: Suntzu]
    #534817 - 01/29/02 11:24 AM (14 years, 10 months ago)

Was your agar lab grade or home brew? If it wasn't lab grade, you might have had some particles in suspension that settled as the agar cooled. As small as they are, you might not have noticed them until looking closely for contaminates. Just a thought....


--------------------
Just another spore in the wind.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* Black Mold! ( Little black dots ) shroomeritus 2,869 4 07/22/01 04:58 PM
by Psilocybe Ryan
* Black Dots/Inoculation Site sawred 1,405 4 07/24/01 02:28 AM
by auto59009
* Black dots?? TheReverend 1,550 6 08/23/01 03:04 PM
by TheReverend
* 3 little black dots SHiZNO 993 5 02/01/04 01:08 PM
by SHiZNO
* black dots
( 1 2 all )
PuffPuffGive 2,506 24 08/06/03 06:46 PM
by PuffPuffGive
* Black dot. SCIOpenEyedDream 724 3 09/29/04 03:11 PM
by MAIA
* Black dots, Is this pin mold? navyseals101 1,947 12 02/10/08 01:20 PM
by navyseals101
* Black dots...
AppalachianGuy
685 4 10/23/08 10:10 PM
by noobieshroomie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
2,204 topic views. 1 members, 1 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.003 seconds on 14 queries.