Home | Community | Message Board

Avalon Magic Plants
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Petri Dish

Jump to first unread post. Pages: 1
Registered: 01/28/02
Posts: 4
Loc: IN
Last seen: 16 years, 5 months
Black Dots Under Mycelium(sp) in petri plates
    #534402 - 01/28/02 10:33 PM (16 years, 5 months ago)

Under the mycellium there's several little black dots they're about the size of lead for a mechanical pencil (.07). you can't see them unless you look from the bottom of the plate, or cut it open as i did when i made some new petri plates (with care to avoid the dots). any help would be great as it what they are and cause. Thanks

Post Extras: Print Post  Remind Me! Notify Moderator
 User Gallery

Registered: 10/15/99
Posts: 1,396
Last seen: 3 months, 3 days
Re: Black Dots Under Mycelium(sp) in petri plates [Re: BD_Man]
    #534520 - 01/29/02 01:09 AM (16 years, 5 months ago)

Are you growing on peroxide or antibiotic media?

Sometimes bacterial contaminants won't prosper in a treated petri dish, but find a nice little nook under growing mycelium. Worst case scenario, but still a possibility, is that it is an anaerobic/capnophilic bacterium that can ONLY thrive in the low O2 environment under the growing mycelium. This would also be consistent with the slow growth rate of the suspect contaminant.
No matter the case, you are 'probably' ok transferring mycelium as long as you stay away from the black points. 'Probably', meaning that the relative size and growth rate of fungal hyphae might allow a few bacterial cells to hitch a ride to the outer growth areas. If it is really an anaerobe, though, this is quite unlikely. If the antibiotic does its job, this is also quite unlikely.
Another likely scenario is that it is just a black precipitate, though such things are usually much smaller than a pencil lead.
If you have some more plates, it might be a good idea to attempt a tiny transfer of periphery tissue [perhaps while making some test inoculum].

**just read, you are doing transfers. . .I bet they'll be fine, take extra care to make it a small amount.

Edited by Suntzu (01/29/02 01:11 AM)

Post Extras: Print Post  Remind Me! Notify Moderator
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 1 month, 25 days
Re: Black Dots Under Mycelium(sp) in petri plates [Re: Suntzu]
    #534817 - 01/29/02 11:24 AM (16 years, 5 months ago)

Was your agar lab grade or home brew? If it wasn't lab grade, you might have had some particles in suspension that settled as the agar cooled. As small as they are, you might not have noticed them until looking closely for contaminates. Just a thought....

Just another spore in the wind.

Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* Black Mold! ( Little black dots ) shroomeritus 3,444 4 07/22/01 04:58 PM
by Psilocybe Ryan
* Black Dots/Inoculation Site sawred 1,586 4 07/24/01 02:28 AM
by auto59009
* Black dots?? TheReverend 1,717 6 08/23/01 03:04 PM
by TheReverend
* 3 little black dots SHiZNO 1,123 5 02/01/04 01:08 PM
* black dots
( 1 2 all )
PuffPuffGive 3,090 24 08/06/03 06:46 PM
by PuffPuffGive
* Black dot. SCIOpenEyedDream 811 3 09/29/04 03:11 PM
* What is the color of mycelium? OCMsurfer 3,108 4 06/27/02 06:25 AM
by OCMsurfer
* Tiny black spot holio1 1,023 2 04/27/04 06:45 PM
by holio1

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Tmethyl, Pastywhyte, bodhisatta
2,457 topic views. 0 members, 5 guests and 1 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Vesp.co, Inc.
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.006 seconds on 19 queries.