Black Dots Under Mycelium(sp) in petri plates - Mushroom Cultivation

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post

Pages: 1

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

BD_Man
Stranger

Registered: 01/28/02
Posts: 4
Loc: IN
Last seen: 21 years, 9 months

Black Dots Under Mycelium(sp) in petri plates

#534402 - 01/28/02 08:33 PM (21 years, 10 months ago)

Under the mycellium there's several little black dots they're about the size of lead for a mechanical pencil (.07). you can't see them unless you look from the bottom of the plate, or cut it open as i did when i made some new petri plates (with care to avoid the dots). any help would be great as it what they are and cause. Thanks

Extras:

Top

Suntzu
Geek

Registered: 10/14/99
Posts: 1,396
Last seen: 1 month, 17 days

Re: Black Dots Under Mycelium(sp) in petri plates

[Re: BD_Man]
#534520 - 01/28/02 11:09 PM (21 years, 10 months ago)

Are you growing on peroxide or antibiotic media?

Sometimes bacterial contaminants won't prosper in a treated petri dish, but find a nice little nook under growing mycelium. Worst case scenario, but still a possibility, is that it is an anaerobic/capnophilic bacterium that can ONLY thrive in the low O2 environment under the growing mycelium. This would also be consistent with the slow growth rate of the suspect contaminant.
No matter the case, you are 'probably' ok transferring mycelium as long as you stay away from the black points. 'Probably', meaning that the relative size and growth rate of fungal hyphae might allow a few bacterial cells to hitch a ride to the outer growth areas. If it is really an anaerobe, though, this is quite unlikely. If the antibiotic does its job, this is also quite unlikely.
Another likely scenario is that it is just a black precipitate, though such things are usually much smaller than a pencil lead.
If you have some more plates, it might be a good idea to attempt a tiny transfer of periphery tissue [perhaps while making some test inoculum].

**just read, you are doing transfers. . .I bet they'll be fine, take extra care to make it a small amount.

Edited by Suntzu (01/28/02 11:11 PM)

Extras:

Top

Seuss
Error: divide byzero

Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 1 month, 8 hours

Re: Black Dots Under Mycelium(sp) in petri plates

[Re: Suntzu]
#534817 - 01/29/02 09:24 AM (21 years, 10 months ago)

Was your agar lab grade or home brew? If it wasn't lab grade, you might have had some particles in suspension that settled as the agar cooled. As small as they are, you might not have noticed them until looking closely for contaminates. Just a thought....

--------------------
Just another spore in the wind.

Extras:

Top

Jump to top

Pages: 1

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

Similar Threads	Poster	Views	Replies	Last post
Black Mold! ( Little black dots )	shroomeritus	4,643	4	07/22/01 02:58 PM by Psilocybe Ryan
Black Dots/Inoculation Site	sawred	2,013	4	07/24/01 12:28 AM by auto59009
Black dots??	TheReverend	2,151	6	08/23/01 01:04 PM by TheReverend
3 little black dots	SHiZNO	1,456	5	02/01/04 11:08 AM by SHiZNO
black dots ( 1 2 all )	PuffPuffGive	4,638	24	08/06/03 04:46 PM by PuffPuffGive
Black dot.	SCIOpenEyedDream	988	3	09/29/04 01:11 PM by MAIA
What is the color of mycelium?	OCMsurfer	3,692	4	06/27/02 04:25 AM by OCMsurfer
Tiny black spot	holio1	1,274	2	04/27/04 04:45 PM by holio1

Extra information

You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,752 topic views. 20 members, 91 guests and 44 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]