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 PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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 black dots 
#1669393 - 06/28/03 11:55 AM (21 years, 9 months ago) |
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I keep getting black dots all over my popcorn jars. I was getting no contams but now every print i make seems contamed. I'm using the bulk neglect tek so that might have to do alittle with it. But the thing that confuses me is that if i dotn pc the jars right away they contam also but if I pc the jars their fine until i innoculate them. Always the same contam. I'm making my prints by wipping tinfoil with alchol and convering with a glass that is also steralized. Any ideas how to stop this? I ordered some syringes but I'm eventually going to need to be able to make prints again.
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Snobrdr311
outdoorenthusiast
Registered: 09/03/01
Posts: 1,468
Loc: Midwest
Last seen: 11 years, 6 months
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Popcorn may be to hard of a substrate, you might not be able to get all the possible contam spores out of the inside of the grain even with pressure cooking. Just stick to the proven methods dude. Use Birdseed or Rye instead of Popcorn.
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 2 months, 29 days
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Use a glove box to make the prints and/or the syringes.
MAIA
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Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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Re: black dots [Re: MAIA]
#1670303 - 06/28/03 09:19 PM (21 years, 9 months ago) |
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I dont think its the popcorn becuase i've used popcorn for a while now with no problems. I never needed a glove box before but i guess it wouldnt hurt.
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YellowPurpleHills
Stranger
Registered: 03/06/03
Posts: 85
Last seen: 22 years, 1 month
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Post deleted by Administrator [Re: PuffPuffGive]
#1671888 - 06/29/03 04:45 PM (21 years, 9 months ago) |
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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thanks for the idea, i'll give that a shot
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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Again with black dots, new jars, new syringes, i'm about to give up, what would be the difference with rye compared to popcorn to prevent contams?
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Anonymous
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as long as you properly sterilized it, doesnt matter.
rye does contaminate with bacteria, a forewarning 
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 2 months, 29 days
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Re: black dots [Re: daba]
#1730494 - 07/19/03 03:53 PM (21 years, 8 months ago) |
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I find popcorn less prone to contams, you should check out some teks.
I don't know how you prepare the rye but popcorn is basically letting it soak for a day, boil in slow flame for an hour, drain , fill jars and PC them. At least that's how i used to do it.
MAIA
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Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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Re: black dots [Re: MAIA]
#1731253 - 07/19/03 10:30 PM (21 years, 8 months ago) |
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Popcorn was duing great for me I. but instead of soaking it I pc it and then put in jars and pc them. But I've made 5 or 6 sets of jars all getting black dots. I dont know whats causing it.
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 2 months, 29 days
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Soak the popcorn for 24 hours, do it in some bleach:water solution.
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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Re: black dots [Re: MAIA]
#1732043 - 07/20/03 10:05 AM (21 years, 8 months ago) |
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That sounds like a good idea, what ratio would you recomend?
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 2 months, 29 days
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1 portion of bleach to 100 portions of water would be ok.
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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Re: black dots [Re: MAIA]
#1732976 - 07/20/03 05:51 PM (21 years, 8 months ago) |
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Ok Thanks, I cant beleive I forgot about that.
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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For some reason popcorn does not want to work anymore for me. I used a glove box, soaked it, new syringes, anything I can think of. Its weird becuase if I pc the jars and let them sit without innoculating them no problems but once innoculated 3 days later allitle white and alot more black dots. I was thinking about going back to vermaculite and rice flour. I mean could the syringes somehow get contamed in the fridge? Their in a zip lock. I have no clue. Moving to a new place in 2 weeks maybe that'll be the solution. Anymore ideas would be appreciated though.
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 2 months, 29 days
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Do you make you own syringes ?
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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Re: black dots [Re: MAIA]
#1773006 - 08/02/03 02:08 PM (21 years, 8 months ago) |
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I have and have done them succesfully but I ordered new syringes to try and eliminate any questions with them. The black dots have occured with the new syringes.
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 2 months, 29 days
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Go with PF Tek, just to be sure.
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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PuffPuffGive
journeyman
Registered: 01/29/03
Posts: 54
Last seen: 21 years, 1 month
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Re: black dots [Re: MAIA]
#1777866 - 08/04/03 10:15 AM (21 years, 8 months ago) |
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ya i think thats the plan, thanks for the help
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GregHimself
Just a guyobssesed withshroomies.
Registered: 07/18/03
Posts: 936
Loc: British Columbia, Canada
Last seen: 7 years, 4 months
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errrrm how big are these black dots...and do they grow at all? Just a thought that it just might be clumping of spores if they are tiny black dots and dont grow at all. Sorry if this is obvious...dont mean to be stupid.
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"The key to successful cultivation is to match the skills of the cultivator with the right strain on the proper substrate under ideal environmental conditions."
Paul Stamets
Growing Gourmet and Medicinal Mushrooms(Third Edition)
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