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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29442200 -

tree frog said:
Okay once more lol







One very cool thing i noticed when looking at these type of crosses; If you follow that exact section under a microscope you should be able to see the exact point where recombination occur.  When i start looking before the cross section meet, you’ll see weird hormonal things happening.  The mycelium will swell and appear to make way for the recombined nucleus, amongst a few other microscopically visible changes.

In my experience if you get pins that form from the hyphal knot groupings,  they are often not the same genetics as the parents, even if they seem to be growing near or on the parent agar wedge.

Edited by trippleblack (12/29/25 11:09 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack] * 1
    #29442280 -

trippleblack said:
tree frog said:
Okay once more lol







In my experience if you get pins that form from the hyphal knot groupings,  they are often not the same genetics as the parents, even if they seem to be growing near or on the parent agar wedge.


I was asking myself that, if the nuclei are transferred "long distance" to the "old" mycelium or not. So if you could really distinguish 12 o clock from 3 o clock mycelium anymore after the fusion. But how do you proof or disproof that for sure without sequencing? Just from looking at the phenotypes?

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Re: Official Copelandia/Panaeolus Thread [Re: shroboo] * 2
    #29442617 -

shroboo said:
trippleblack said:
tree frog said:
Okay once more lol







In my experience if you get pins that form from the hyphal knot groupings,  they are often not the same genetics as the parents, even if they seem to be growing near or on the parent agar wedge.


I was asking myself that, if the nuclei are transferred "long distance" to the "old" mycelium or not. So if you could really distinguish 12 o clock from 3 o clock mycelium anymore after the fusion. But how do you proof or disproof that for sure without sequencing? Just from looking at the phenotypes?


Mechanically the nucleus can travel back through port holes at the septa, i have no idea if that work in a real time scenario with what we are discussing.  Staining and chasing a nucleus around with a microscope sounds like a fun thing to look into.  I also ponder over the aerial mycelium, if a plate is well colonized, lot’s of things may get mixed up beyond just simple growth on a 2d surface. 

If you are very familiar with a particular dikaryon, Simply looking at the phenotypes is a good start.  I like plating the newly formed siblings next to each other and next to the parents on a new agar plate to check for original cultures.  Potency and or alkaloid testing is another good marker for analysis.

I plan to get into sequencing, but have not made that investment yet.

Edited by trippleblack (12/30/25 04:57 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack] * 9
    #29442835 -

Third year growing this Wild Coast. Loving the different phenotypes across the trays.


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Re: Official Copelandia/Panaeolus Thread [Re: old_fart_out_west] * 6
    #29443012 -

Panaeolus cyanescens var. Blue Springs red spore



old_fart_out_west, Wild Coast are amazing! They always been very potent and visual to me. What's your experience with them?


--------------------
Ps. Serbica official thread - serbica connoisseur :mushroom2: :smirk:
πŸ“¨ Journey of a Letter βœ‰οΈ the thread about how letters travel, how to address an envelope, and how to expedite delivery πŸ“¨πŸ“­
☞ Guide to the Shroomery - how to grow shrooms from A to Z guide ☜
If you want to dive in and grow some Pans, this is the place to start! β₯Ύ
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Bra] * 10
    #29443034 -

Happy Happy Pan-people!



--------------------
Shrumonoids

Panaeolus Cambodginiensis from Russia
Panaeolus Cyanescens Jamaica from Russia
Goliath.A new greenhouse.Customization

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Re: Official Copelandia/Panaeolus Thread [Re: angamshroom] * 3
    #29443040 -

Bra you're bispo Washington genetics just went into the fruiting chamber

It went spore plate, t1 - AIO bottles (thanks tree frog) - fruiting conditions

Phv spore plates are well overdue, but they're next.

I had 2/5 AIO jars contam. I'm just inexperienced and I'm trying to rule out vectors.

Tree Frog your AIO jars are epic thanks so much, and your casing Tek is very straightforward.

Kloakk thanks for just always being there when I needed help. Now we wait, hopefully these turn out better than my last attempt.


--------------------
I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

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Re: Official Copelandia/Panaeolus Thread [Re: el boso] * 7
    #29443074 -

I'm going to redo my AIO tek soon.  As I'm playing with it again recently :smile:

Not AIO, but really loving this Pan from Aguadilla, PR.



Thanks to Mr :yields: for finding the variety.  Because they put out some meaty frickin pans!


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/31/25 09:18 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 12
    #29443287 -

Photo dumping.  Four spored basidia.  Translucent violet spores.














--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29443323 -

Aguadilla, beautiful mushrooms and beautiful place, great job Tree Frog!


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer] * 11
    #29443760 -

Panaeolus cyanescens var. Blue Springs red spore






--------------------
Ps. Serbica official thread - serbica connoisseur :mushroom2: :smirk:
πŸ“¨ Journey of a Letter βœ‰οΈ the thread about how letters travel, how to address an envelope, and how to expedite delivery πŸ“¨πŸ“­
☞ Guide to the Shroomery - how to grow shrooms from A to Z guide ☜
If you want to dive in and grow some Pans, this is the place to start! β₯Ύ
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
    #29443767 -

my TTBVI has grown a large amount but its not good looking. I dont think its contamination it may be because it was from agar clone multiple times I purchased from someone. I was still going to try growing it but I can't afford a grow tent I just mist other mushrooms in tubs. I seen pretty neat automatic monotubs but its still pretty expensive to build.

I was thinking about getting spores and starting on agar fresh try 12oz LC grow and some A2G at the same time but im worried about the difficulty fruiting.

Im planing on using hydrated lime and cold water pasturize straw. Then I will heat black cow to 170F in a oven for 2 hours and mix in straw and use that as substrate.


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Official Copelandia/Panaeolus Thread [Re: CaptainTrips420] * 1
    #29443829 -

Why not pasteurize the straw and manure together?


--------------------
Tent for Tropical Species

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Re: Official Copelandia/Panaeolus Thread [Re: CaptainTrips420]
    #29443832 -

This worked well for me and is fairly inexpensive.  It's also as simple to operate as a monotub really, just spray the walls down once a day.

https://www.shroomery.org/forums/showflat.php/Number/29419466#29419466

You can also do monotubs.  You've seen baba's thread?

https://www.shroomery.org/forums/showflat.php/Number/27637997/page/1


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 4
    #29443861 -

Well, I've decided that I for sure like BVI! Had my first trial dose (~280mg) and wowie-zowie, that was just what I had been needing! Wasn't so much that I got carried away, but it provided me a *really* nice introduction to what they can do.

Bisporus has been enthusiastically recommended to me & is next on my grow list, so I am looking forward to trying them out.


--------------------
Peace to you, friend!
The HHG is a guide to getting started.
New User Welcome Wagon
GROWER’S BEST photo contest: join the fun!
CAKE WARS III - Starts SEPT 1, 2026

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Re: Official Copelandia/Panaeolus Thread [Re: flapping_bird] * 1
    #29443905 -

I really just find it interesting that Panaeolus contains psliocybin. I only have ever found Panaeolus foenisecii in the wild and always thought they look neat I eaten them a few times for 5-HTP it helps with insomnia.

If my TTBVI doesn't grow for whatever reason I will try spore to agar instead of agar clone but other clones I purchased preformed really well.


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Official Copelandia/Panaeolus Thread [Re: CaptainTrips420] * 10
    #29444230 -

a couple estero cyan pics.  got to go back in time for Ihese:smile:

and bvi bag, not a failure technically.  the estero bag hasn't done much yet either.




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Re: Official Copelandia/Panaeolus Thread [Re: stagger] * 11
    #29444239 -

Harvesting estero this very second.  What i love about Estero is the fruits are dense like chopsticks, potency nearly up there with the bvi i tested.   



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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack] * 9
    #29444334 -

Panaeolus cyanescens var. Blue Springs red spore. Printed. The 2nd flush incoming.


--------------------
Ps. Serbica official thread - serbica connoisseur :mushroom2: :smirk:
πŸ“¨ Journey of a Letter βœ‰οΈ the thread about how letters travel, how to address an envelope, and how to expedite delivery πŸ“¨πŸ“­
☞ Guide to the Shroomery - how to grow shrooms from A to Z guide ☜
If you want to dive in and grow some Pans, this is the place to start! β₯Ύ
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: trippleblack] * 14
    #29444401 -



Second flush.  Caps stayed tiny.  But I love the cream color and think I can get some nice coverage from these.

I'll probably remake and keep the f0 of this as I have both the parents.  Maybe some day I'll have time to run out more generations.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Jump to top Pages: < First | | 2001 | 2002 | 2003 | 2004 | 2005 | 2006 | 2007 | 2008 | 2009 | 2010 | 2011 | 2012 | 2013 | 2014 | 2015 | 2016 | 2017 | 2018 | 2019 | 2020 | 2021 | | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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