fahtster said:

First and second flush (respectively) of liquid amber in a 4oz jar.. this jar was grown in my jcm


Some citrus ridge.. this was grown with just the sandwich bag top with holes poked in it. 


Obv it needs tweaking.  It needs more air but that’s tough to do without drying the tiny sub out.  My fix plan is to wrap h2o2 wetted paper towel around the jar inside the bag to help keep the rh up so I can add more holes or slits to the bag for when the cycle fan comes on.  The h2o2 should help keep the paper towel clean and evaporate into harmless water and o2.

We’ll see if it works.

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Mackload said:

Where did you read these things, or think you read these things, or read things that went both ways recently? Idle curiosity

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tultedspoon said:

Is there a Panaeolus strain that needs to breed twice before regular fruits show up? I thought I read something along these lines, I read something interesting the other day regarding microscopic fruits forming and dropping spores, and I'm wondering if there is a species that has mycelium recombining first, then microscopic fruits, and then regular fruits.

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I think you are mixing up a few key processes.

Most spores germinate with a single nuclei (a monokaryon).  They need to find another monokaryon that has a compatible mating type in order to reproduce.  When they do they clamp and form a dikaryon (single cell with two nuclei). And generally this mated strain doesn't genetically recombine until it makes spores in the fruit body.

However, mycelium also has what is called a parasexual cycle.  Take the dikaryon above, and rarely (like 1/1000 times) when it meets another dikaryon, it will donate a nuclei.

But a cell can't have three nuclei, so what happens is one of the nuclei recombines.  And you now have a dikaryon with half the DNA of one spore, and a quarter of the DNA from two other spores.

And even though I said rarely, keep in mind that a multi-spore syringe can have a lot of spores in it. Which means there's a lot of opportunities for parasexual crosses to happen.

RR was under the Western assumption that a good isolate (which is two nuclei that never undergo a parasexual cycle) can be superior to any heavy MS inoculation that has undergone parasexual breeding.  But also admitted that finding such a isolate can take a lot of work. Meaning you might test 10 or 20 of them before you find that great one.

On the other hand, mycologists in Asia prefer heavy MS strains.  Believing that myceliym that has undergone many parasexual cycles is better suited for cultivation.

Personally, I don't worry about it too much. I take multiple transfers off of a germination plate to a single plate. And the one that runs the fastest I use. If I noticed any fruit bodies forming especially from parasexual crosses, I clone them off the plate and run those too.

The only time I worry about avoiding breeding on a plate, is if I'm trying to isolate a monokaryon to cross varieties from different locations.

Edit:  You can see on the top right of this plate where a parasexual cross happened between these two dikaryons.  That culture on the right is also knotting on the plate, so I will use this one.

tree frog said:

Edit:  You can see on the top right of this plate where a parasexual cross happened between these two dikaryons.  That culture on the right is also knotting on the plate, so I will use this one.

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