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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29419794 -

Yes plenty of folks will put more then one per plate or buy divided plates


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #29419812 -

I dont see how that would help except if ur trying to have plates as fast as possible. It will just fill the plate faster. Im trying to find the most symmetrical growth possible and transfer those to new plates.


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29419819 -

It looked like it worked out in your favor though, maybe if you did that for all your plates you would have twice as many good mycelium waves. Question does mycelium care what's happening 180 degrees from a transfer? If it's not symmetrical full circle does that mean the plate isn't as good? or is there other factors that could be at play like available agar nutrients in a certain spot, or microclimate in the petri (microscopic condensation on one side of plate, maybe your table or shelf isn't 100% level).

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29419843 -

I know what you mean but it shouldnt take THAT many plates to find some nice symmetrical plates from a clone allready. Could be good if ur doing some transfers from spores and need to transfer more quickly.

Also sometime it can take a bit longer where it needs to grow bigger to show if its truly symmetrical. I dont want them merging with each other too early.

With my 4 good plates Ill transfer them to like 20 plates and then let the best one grow and transfer some to grains and the rest will go in the fridge for longer storage and future transfers.


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris] * 1
    #29420010 -

I was surprised to see t2 bisporus looking so orderly. is there an explanation within the species?



whereas ttbvi t2 has still got a rather differentiated expression. would you go to grain with this?



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the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

Edited by chris77 (12/05/25 05:15 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29420077 -

Anomalocaris said:


Here they are. What do you think is going on with the not as nice looking ones? They are all transfers from a couple of nice looking and symmetrical plates. I have an FFU and sterilise my cork borer in PC to make transfers and flame it outside my FFU every about 3 transfers.

Either theres some bacterias riding along somehow or its just how some pan cyan grows im starting to think.

And/or maybe they also dont like charcoal that much?


Sectoring.  The charcoal is causing the mycelium to sector more.

Very cool :smile:  How much charcoal did you use?


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29420191 -

If I have a martha, would it be a bad idea to have a small fan running all the time and have the humidifier running all the time so that humidity is constantly high and there is a lot of FAE? Isn't that what Pans prefer?

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29420205 -

tree frog said:
Anomalocaris said:


Here they are. What do you think is going on with the not as nice looking ones? They are all transfers from a couple of nice looking and symmetrical plates. I have an FFU and sterilise my cork borer in PC to make transfers and flame it outside my FFU every about 3 transfers.

Either theres some bacterias riding along somehow or its just how some pan cyan grows im starting to think.

And/or maybe they also dont like charcoal that much?


Sectoring.  The charcoal is causing the mycelium to sector more.

Very cool :smile:  How much charcoal did you use?


I used 4g of activated charcoal in 400 ml of water


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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon] * 3
    #29420233 -

tultedspoon said:
Asura always a pleasure when you chime in. I read for several months before posting researching pans exclusively, and some of your advice is up there with RR.


Well, if I have ever given you or anyone else advice, I do apologize. I prefer to just talk about what I do and show the results I am getting.

And everything I think I know, probably goes back to RR in some way :smile:


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Cultivating Panaeolus cyanescens


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Re: Official Copelandia/Panaeolus Thread [Re: chris77] * 1
    #29420294 -

chris77 said:
I was surprised to see t2 bisporus looking so orderly. is there an explanation within the species?




It germinates with two nuclei instead of one.  Which has a lot of effects on how quickly the gene pool changes, as well as how much genetic diversity there is in a streak or a transfer.  As well as their preferences for outcrossing.

I don't know if that's specifically why  they organize so quickly.

It could also be nutrient related, maybe it sectors less because it likes or dislikes the nutrients :shrug:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29420510 -

how you know dat shit


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris] * 1
    #29420518 -

:rollsafe:

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Re: Official Copelandia/Panaeolus Thread [Re: Asura]
    #29420546 -

Asura said:
tultedspoon said:
Asura always a pleasure when you chime in. I read for several months before posting researching pans exclusively, and some of your advice is up there with RR.


Well, if I have ever given you or anyone else advice, I do apologize. I prefer to just talk about what I do and show the results I am getting.

And everything I think I know, probably goes back to RR in some way :smile:


Some people on here think that everything is some form of advice or manipulation on here and they got me using the terminology improperly now. I guess some people get triggered when others speak, prob just some type of mental disorder.

I appreciate your contributions I mean and detailed writeups! I don't feel threatened by your words being "advice", but your scientific contributions and experiences are worthy I guess I am trying to say.

Recently stumbled onto tregar and am looking at some of his genius as well.

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris] * 2
    #29420992 -

Anomalocaris said:
how you know dat shit


I have a copy of this book.  Much of it is available online.  This part discusses heterothallic and homothallic sexuality.

https://davidmoore.org.uk/21st_Century_Guidebook_to_Fungi_PLATINUM/Ch08_01.htm

I've also read a lot about pseudo homothallic (two spored) mushrooms on Wikipedia and in research papers.  Because I have a lot of passion for pan bisporus in particular.

My theory though is that it would be more disorganized on a different media.  (Maybe charcoal?)



This is mystery sa.  Sectors heavily into vegetative and fruiting strains on my media recipe (0.5% lme).  It's also two spored/pseudo homothallic.



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29421204 -

Cool stuff.


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29421209 -

I have a few grain spawn of ttbvi that are almost fully colonized but they have a few spots with a couple of grains that seem wet that just wont colonize. Is it ok to spawn these jars anyway?


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29421290 -

Pick the uncolonized ones out, because that's a fun place to live if you're mold spores trying to germinate.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/06/25 01:37 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29421307 -

Gonna be a bit hard to find them once I shake them before spawning :frown: but I guess ill try


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29421326 -

Oh, I was thinking dry grains on top.  If stuff won't colonize lower down it's possibly bacterial or just wet from condensation.  You can shake it and see if it recovers, top fruit it, or spawn it and wish for the best.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/06/25 02:05 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 4
    #29421452 -


Oops forgot I did weza bags ...I'm not even close to ready 😂 , no manure no tub set up


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras: Filter Print Post Top
Jump to top Pages: < First | | 1992 | 1993 | 1994 | 1995 | 1996 | 1997 | 1998 | 1999 | 2000 | 2001 | 2002 | 2003 | 2004 | 2005 | 2006 | 2007 | 2008 | 2009 | 2010 | 2011 | 2012 | | Last >

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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