phytophthora said:

Soo… how long would we venture bisporous spawn is viable for? Appreciate everyone’s perspective!

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Anomalocaris said:

tree frog do you also get irregular growth patterns on your pan cyan agar? Some of my plates are very symmetrical and others start with thicker mycelium then becomes thin mycelium at random spots. Ive seen others that seem to get weird patterns like that too.

Is it just how pan cyan on agar acts?

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blueprint said:

Thank you!

How many Flushes you make normaly with Pan's?

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PuffM4gicDr4gon said:

Anyone notice Pan mycelium get puffy after a cabin sequester?

I'm not at home, so I'll post pictures later in the week.

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With cyans yes.  Here's bbar and weza.



But pan mycelium can vary a lot across species.  Undescribed African Pan I'm still trying to fruit and olivaceous.

tree frog said:

I tend to see some variability in density.  But if it's a lot it could be sectoring or bacteria riding along.

Either way I select for organized and vigorous growth.  This is heavy MS so a bit disorganized.





The colony on the left is slow, so I will take wedges from these three spots.

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Here is some of my plates. This is ttbvi. Notice how top left, top right and bottom right have some thin mycelium that starts growing.



Also this one.

Do you think its caused by bacterias?

tree frog said:

It's probably just sectoring.  Mycelium will do that without bacteria.

I'm just saying given the choice between organized growth and not, for inoculation take the organized growth.

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Krs_bmx said:

hardhead43 said:

Pan Cambos are very forgiving and easy to grow.  Had a great trip on 1.6g dry of these bad boys.  These do well in a seed tray with humidity dome.  Easier to harvest than ttbvi because they are much larger fruits.

These tolerate heavy misting twice daily as well.  Real troopers these things.

And you better put your seat belt on, I'll tell you that much.  - Chong.

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Hey bro do you have any advice on how to grow these. I’ve tried ttbvi and I keep failing. Do I need to get a tent and have the temp up to 78-80°F and a humidifier keeping it at 90% like I’ve seen people say. I’ve seen people just do normal tubs on a shelf and they grow but mine keep failing

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hardhead43 said:

Krs_bmx said:

hardhead43 said:

Pan Cambos are very forgiving and easy to grow.  Had a great trip on 1.6g dry of these bad boys.  These do well in a seed tray with humidity dome.  Easier to harvest than ttbvi because they are much larger fruits.

These tolerate heavy misting twice daily as well.  Real troopers these things.

And you better put your seat belt on, I'll tell you that much.  - Chong.

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Hey bro do you have any advice on how to grow these. I’ve tried ttbvi and I keep failing. Do I need to get a tent and have the temp up to 78-80°F and a humidifier keeping it at 90% like I’ve seen people say. I’ve seen people just do normal tubs on a shelf and they grow but mine keep failing

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I have been using seed trays 10x20 with 7 inch humidity domes.  I have also grew ttbvi in shoebox.  Look for fastest growing "wispy" (not matted up thick) genetics on agar. 1 or 2 transfers is enough if things look clean.  Make sure you have clean spawn.  I like millet or WBS that is mostly millet due to larger number of inoculation points which makes for faster colonization of substrate.  Sure that other grains would be fine.  Pasteurized 4:2:1 coir:black kow:vermiculite  +/- gypsum for substrate.  I use 1 mycoquart of spawn to 3 quarts of substrate.  I have been just dumping the mycoquart into the bag and resealing it so I can mix the heck out of it without touching it.  Then either let is colonize in bag, break it up and then dump in tray....or just dump it in tray and let it colonize in the tray.  Only advantage I see to letting it colonize in bag is you are delaying introduction of room air contaminates until substrate is fully colonized.  You only have the mycelium and whatever survived pasteurization. Plus with it being fully colonized on transfer to the tray, you can go ahead and case it.  Pasteurized field capacity jiffy mix works great and is easy to prep.  To pasteurize, I just put my bags of substrate or casing into plastic bags (unicorn for substrate) and vacuum bags for casing.  Ziplocks would be fine for casing. Ziplocks would be fine for substrate if you weren't going to try to colonize in the bag.  Place in water bath at 160 degrees for 3 hours and move material around in bag several times throughout to achieve even heating.

I open vents on the humidity domes from the beginning now and just mist the jiffy mix when it starts looking dry (lighter color) which ends up being once or twice daily.  Also spray top and sides of dome when misting.  After pinning starts, leave vents open and cock the lid a bit to increase airflow more. 

Genetics matter.  I have probably a 50-66% success rate with ttbvi and pan cambos, with pan cambos being the more successful of the two.  I don't get super dense canopies like many of the better growers on here, but I just harvested 175g wet off that pan cambo tray pictured above.  That will be about 12g freeze dried which equates to about 7 or 8 very intense experiences (1.6g dry will rock your world)

PM with any specific questions. Keep trying different genetics until you find something that will grow in conditions you provide,  also change up your technique based on tips on here if you are getting repeated failures...  keep plugging away at it.

Going back to spores can help if you end up with crappy genetics.

Oh.  I have not been running humidifiers, just misting.  Room temp set to cool at 82 and heat at 78 (mini split).  I've had them fruit outside this temp range.  Warmer better for pans I think though.

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