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Re: Official Copelandia/Panaeolus Thread [Re: shroboo]
    #29408040 -

Some folks sterilize casing and do well with it, I don't suggest sterilizing anything that will be exposed to open air prior to full colonization personally.

Even a contamination resistant substrate like coir can change enough by being cooked to allow molds to germinate.  I tried this recently in fact after remembering an old Pasty grow doing that.

My cooked coir contaminated prior to spawn just from mold spores in the house.

Anyway, cyans don't really require bacteria to pin.  So open air inoculation aside, a sterile casing would be as good as a pasteurized one for that species as far as I know.

Not sure on others but my suspicion is the bispo 'clad' is more interested in microbial interactions, even if all strains aren't dependent on them.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (11/21/25 08:56 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29408059 -

tree frog said:
Some folks sterilize casing and do well with it, I don't suggest sterilizing anything that will be exposed to open air prior to full colonization personally.

Even a contamination resistant substrate like coir can change enough by being cooked to allow molds to germinate.  I tried this recently in fact after remembering an old Pasty grow doing that.

My cooked coir contaminated prior to spawn just from mold spores in the house.

Anyway, cyans don't really require bacteria to pin.  So open air inoculation aside, a sterile casing would be as good as a pasteurized one for that species as far as I know.

Not sure on others but my suspicion is the bispo 'clad' is more interested in microbial interactions, even if all strains aren't dependent on them.


Thanks for pointing that out.

My assumption was regarding the general contamination risk. If the grass seeds with their microbes might lower the contam risk of the casing, no matter if sterilized, pasteurized or out of the box? Independently from the pans. Just a thought, don't know if that makes sense.

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Re: Official Copelandia/Panaeolus Thread [Re: shroboo]
    #29408067 -

I have yet to have a properly pasteurized casing contaminate on me prior to late flushes.

To give you an idea.



This was after the fourth flush. And a slime mold decided to live here. Still no green.

It's almost always the spawn that costs me projects before the third flush.

But I don't sterilize my casings so I don't know. But I don't see any way to inoculate a sterile casing with grass seed without exposing it to mold spores too.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29408086 -

yea mold would sporulate at the same time of course.

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Re: Official Copelandia/Panaeolus Thread [Re: shroboo] * 3
    #29408662 -



TTBVI plates. Are the pac-man looking ones top left, top right and bottom right caused by moisture making the mycelium thinner or can it be contam?


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29408690 -

Anomalocaris said:


TTBVI plates. Are the pac-man looking ones top left, top right and bottom right caused by moisture making the mycelium thinner or can it be contam?


The 4 best plates could knock up +30 jars, use them :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29408693 -

fahtster said:
I don’t even bother with anything other than wa for cloning anymore.  It’s too reliable.  Like, works every time kind of reliable.  I do take em to a CS after they’ve grown out but I would do another transfer anyway, might as well be to a CS


Have you tried clone straight to cabin? That’s what I’ve been doing lately and works well not quite 100% success but close to. Pulling from top of cabin could let you skip a step. But I back WA too just curious if you tried.

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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk]
    #29408694 -

Kloakk said:
Anomalocaris said:


TTBVI plates. Are the pac-man looking ones top left, top right and bottom right caused by moisture making the mycelium thinner or can it be contam?


The 4 best plates could knock up +30 jars, use them :rockon:



I know but a lot of some other plates I made also have that pattern with thick and thinner mycelium at other places. Im mostly wondering if I screwed up a bit in my technique and its some hidden contam.


--------------------

Edited by Anomalocaris (11/21/25 08:14 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29408703 -

kirkeng said:
fahtster said:
I don’t even bother with anything other than wa for cloning anymore.  It’s too reliable.  Like, works every time kind of reliable.  I do take em to a CS after they’ve grown out but I would do another transfer anyway, might as well be to a CS


Have you tried clone straight to cabin? That’s what I’ve been doing lately and works well not quite 100% success but close to. Pulling from top of cabin could let you skip a step. But I back WA too just curious if you tried.


WA cabin for clones :strokebeard:

Anomalocaris said:
Kloakk said:
Anomalocaris said:


TTBVI plates. Are the pac-man looking ones top left, top right and bottom right caused by moisture making the mycelium thinner or can it be contam?


The 4 best plates could knock up +30 jars, use them :rockon:



I know but a lot of some other plates I made also have that pattern with thick and thinner mycelium at other places. Im mostly wondering if I screwed up a bit in my technique and its some hidden contam.


Hard to say, and if you're into knowing, then keep at it, might find something interesting.

:havesomescience:

:superscience:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 2
    #29408714 -

Kloakk said:
kirkeng said:
fahtster said:
I don’t even bother with anything other than wa for cloning anymore.  It’s too reliable.  Like, works every time kind of reliable.  I do take em to a CS after they’ve grown out but I would do another transfer anyway, might as well be to a CS


Have you tried clone straight to cabin? That’s what I’ve been doing lately and works well not quite 100% success but close to. Pulling from top of cabin could let you skip a step. But I back WA too just curious if you tried.


WA cabin for clones :strokebeard:



I don’t know if we’re ready for someone to harness so much power…

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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #29408724 -

kirkeng said:
I don’t know if we’re ready for someone to harness so much power…


:rofl:

I told Faht I'd try it the next time I was cloning, but haven't tried it yet. But as I recently started my Semilanceata project, and did my first wild fruit cloning. Lord did I get my ass kicked, I took about 50 clones from 10 different fruits, 90% was so bacterial that I couldn't salvage any of it, the last 10% was 3% luck and 7% water agar plate :lol:

So that got me thinking that combining wild fruit cloning with AW cabin might be cool :strokebeard:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 1
    #29408745 -

It’s a good idea I think, gotta do anything you can to tip the scales in your favor! Good luck in the project, honestly I might have to bother you for a trade for that. I’ve been having my mom hunt for me all season but she’s older and not having much luck, I have no idea when I’ll be able to go back and visit her either.

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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #29408800 -

kirkeng said:
It’s a good idea I think, gotta do anything you can to tip the scales in your favor! Good luck in the project, honestly I might have to bother you for a trade for that. I’ve been having my mom hunt for me all season but she’s older and not having much luck, I have no idea when I’ll be able to go back and visit her either.



Just say the word man, got some small, faint prints, that did germinate, and I got some wedges, just hit me with a :pm: and I'll help the best I can. You know, to spare your mother and all :rofl:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 2
    #29408994 -

How are my agars looking this is T1 from spore - it's very front and these pictures represent 16 total that all look like this.








--------------------
I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 2
    #29409040 -

Kloakk said:
kirkeng said:
I don’t know if we’re ready for someone to harness so much power…


:rofl:

I told Faht I'd try it the next time I was cloning, but haven't tried it yet. But as I recently started my Semilanceata project, and did my first wild fruit cloning. Lord did I get my ass kicked, I took about 50 clones from 10 different fruits, 90% was so bacterial that I couldn't salvage any of it, the last 10% was 3% luck and 7% water agar plate :lol:

So that got me thinking that combining wild fruit cloning with AW cabin might be cool :strokebeard:


I wonder if the inside of wild fruit is sterile if you avoid edge contaminates......  Perhaps wiping down outside of fruit very lightly with alcohol or lysol might be helpful for the below procedure, but I haven't found it helpful with indoor grown fruits.

Let me tell you what I've been doing for cloning that has been working well for me so far.  My main issue was how to I get a clean section. I've tried wiping down with isopropyl, cutting stem with red hot scalpel....all a bunch of pain in the butt...


Work in front of flow hood.  (SAB would be fine too I think.)
Flame sterilize tweezers and set them in front of hood on sterile surface.  I wipe everything in front of hood down with alcohol or lysol before starting.
Cut cap and base off stem of fruit.

Start at one end and pull the stem apart.  For pans this has worked well and usually rips all the way down leaving you with 2 semicircles if you look at it end on.  Middle is as sterile as it is going to be.

Now...here is the key part.  Bend the stem away from the flat part.  In the middle of the bend, the very middle usually will break up and start sticking up like a splinter on a piece of broken wood. 

Now you can take your sterile tweezers and grab the base of this "splinter" and pull it out without touching the edges of the stem.

Transfer this immediately to agar and you are done. 

I got 3/4 clean plates last time I did this and pretty sure the one that contammed was me touching something with the tweezers.

Sometimes splinter sticks to tweezers.  You might want to have something to help get it off tweezers, but I usually just stuff it into the agar so i don't have to use both hands. Tweezers in one hand and petri lid in other hand.  I don't like sitting the lids down.  Think I've had some edge contams from sitting the lid down.  I like to work fast and not wipe things down a whole lot and not as careful as I could be sometimes.  If I had higher contam rates, I would be more careful.


--------------------
I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

Edited by Hardhead (11/22/25 08:21 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead] * 5
    #29409214 -

This has pictures to go along with what you’re describing

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29409611 -

Is charcoal agar fine with pan cyan?


--------------------

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris] * 13
    #29409813 -

First chlorocystis attempt




--------------------
what i've been growing

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Re: Official Copelandia/Panaeolus Thread [Re: arlo.arlo] * 8
    #29409894 -

Nice.  Couldn’t get my chloros to fruit.  Tried a few times.  Those are pretty

Recent pics

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29409919 -

Bisporus staying like this for about two weeks now. Unmodified tub. No knots yet. Should I be more patient or recase? I thought of recasing with grass seeds as treefrog decribed some posts ago.


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Jump to top Pages: < First | | 1983 | 1984 | 1985 | 1986 | 1987 | 1988 | 1989 | 1990 | 1991 | 1992 | 1993 | 1994 | 1995 | 1996 | 1997 | 1998 | 1999 | 2000 | 2001 | 2002 | 2003 | | Last >

Shop: North Spore Bulk Substrate   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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