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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris] * 1
    #29377528 -

I'll have to post a picture tomorrow but I found yet another Panaeolus species growing where my normal flushes grow.

I can't tell if it's a Panaeolus cyanescens wild print south africa or the australia grasstree version. The stem is white and it bruised where I pinched it I'm takin a print.

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris] * 4
    #29377932 -

Yup.

Standard amount of agar agar.

0.5% full nutrient plates
500ml filtered water
10g agar
2.5g lme

Here's a bvi back cross on the recipe.



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29378011 -

I am having the darndest time getting Pan Cyan Thai to pin.  I have played with the temp between 75-80F (is there a real spot I need this at), I have my FAE set to clear the grow area every 15 minutes and I have my humidity at 95-99% (is there a perfect, this actually is sometimes as low as 85-99 as a fluctuation), I also mist.  Where do I need to tune in more?  I am not getting any pinning and this has been a problem in the past (Rye to Manure, no contams as well as PFtek cakes with manure and just no pins until they eventually contamed with Trich).

Not sure what else I can do.  I have also tried Ryza pods (I am testing them now) and while they colonized, I have not been getting pins with them either (1 week so far since switching to fruiting chambers).

Is my mistake that I'm working with Suphanburi/Thai?  I did these as MS, do I have to have an isolate on Agar?

I don't know what else I should be doing to improve my results.

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29378214 -

Treefrog are your petri dishes vented or non-vented? I want to get into agar and was just wondering what kind of dishes people use here mostly.

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon] * 1
    #29378565 -

Not vented.  They're glass and wrapped in grafting tape.

@IamAlot, there's no perfect temperature.  And different strains of cyans prefer a bit higher or lower.  I keep my fruiting chambers around 77f.

For humidity, I keep the humidity very high until pins set using a flipped lid over the tray in my fruiting chamber.  Probably 95-99 rH in the tray.  Very little or no misting needed.  Once the first flush pins set, I take the lid off the rest of the grow.

Conditions are like 90-80 rH in the chamber for good evaporation and cap development.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29378643 -

A question: Do you all pasteurize your substrates? I'm switching from tubes to pan.cyan. My first attempt with cow manure, coconut mix, and MIB strain was successful. And that was it. Since then, everything has gone wrong, and when I recently tried alfalfa, sterilized, I had a 100% contamination rate. It's slowly getting frustrating. I had always read that sterilization actually opens the way for contamination. Is that true? I don't really dare to do it, I'm afraid to just pasteurize. Nevertheless, I want to try it, once with pellets of straw and alfalfa, and once I thought I'd make a manure-coconut substrate in a container and put it in the oven. With an Inkbird or meat thermometer, I can then check that the temperature stays at 70 degrees inside for 4 hours. Would this be a way to go?
To document my steps and possibly get more professional help, would this be the right thread to post in and get support? Thank you for any answers, dear mushroom community.


--------------------
Go deeper and take more!!!

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Re: Official Copelandia/Panaeolus Thread [Re: Maormuisir] * 1
    #29378650 -

@Tree Frog - Thank you.  I just moved my temp from 74.5 or so to 77 and I'll keep it there and work on leaving the humidity up high until pinset and not mist.  I appreciate you and your help <3

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Re: Official Copelandia/Panaeolus Thread [Re: Maormuisir] * 1
    #29378675 -

Strawboy is using 10% alfalfa and 90% coir by dry weight.  His prep is pretty simple if you search his name in this thread you should be able to find it.  But he's basically taking alfalfa pellets and putting them in boiling water and then hydrating his coir.

Pasteurization works well with some things, but if something has a lot of nitrogen in it, I'm not sure if that applies to alfalfa, pasteurization might not be enough.

Sterilization on the other hand can basically work with most things, but you have to do your inoculations in a way where you are not introducing mold spores.  Which means in front of a flow hood or in a still air box, some people can get away with it open air but they really know what they're doing.

140f for an hour or longer it's what I usually recommend to people. You can go much longer, I have pasteurized things overnight. You're essentially composting. Developing a thermophilic microorganism profile, that will then go dormant at room temperature. But because they've colonized the substrate, there's no space for contaminants to germinate.

That said, if your spawn is dirty or wet, or not fully colonized, when you introduce it to a pasteurized substrate in open air you are still going to have problems.  I think this has personally been an issue for me, I switched to larger grain jars which makes my grains too wet and a vector for contamination.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: IamAlot]
    #29378677 -

Happy to help!  I hope they work out for you.  But yeah, knots forn well at like 98 humidity. A lot of folks will have strategies to manage that, either misting the surface or using high lipped trays.  Aggressive fog cycles can do it too, essentially using the fogger to mist.

I gave up on all of that, I had too many trays stall from overmisting or under misting. And found that a flipped lid works just fine.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 6
    #29379038 -

My first attempt clone samples have grown out pretty good on the lme agar. I'm going to make a transfer to h2o agar tonight just in case.




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Re: Official Copelandia/Panaeolus Thread [Re: Centerfinger]
    #29379125 -

Nice

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29379167 -

Thanks, I'm super impressed that it went as well as it did. I will admit I skipped the hydrogen peroxide wipe. I was in a hurry and it was a last minute, "hey I should clone that nice looking cluster" vibe.

Who knows if my LI's will work next time. All in all, my fist stab at pans generated some fruits so I feel blessed.

I should write up a trip report, my 2.75g was great, but totally different than my 10g cube trips. The pans wanted me in nature and the visuals were more tight patterned if that makes any sense.


--------------------




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Re: Official Copelandia/Panaeolus Thread [Re: Centerfinger]
    #29379179 -

Centerfinger said:
Thanks, I'm super impressed that it went as well as it did. I will admit I skipped the hydrogen peroxide wipe. I was in a hurry and it was a last minute, "hey I should clone that nice looking cluster" vibe.

Who knows if my LI's will work next time. All in all, my fist stab at pans generated some fruits so I feel blessed.

I should write up a trip report, my 2.75g was great, but totally different than my 10g cube trips. The pans wanted me in nature and the visuals were more tight patterned if that makes any sense.


The peroxide wipe is mainly for ffu/hoods (at least that’s the thinking).. it’s just so the flow doesn’t blow stuff on the outside onto the inner tissue.  Probably not a huge issue either way.  Glad it’s working out for ya :thumbup:

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29379438 -

tree frog said:
Happy to help!  I hope they work out for you.  But yeah, knots forn well at like 98 humidity. A lot of folks will have strategies to manage that, either misting the surface or using high lipped trays.  Aggressive fog cycles can do it too, essentially using the fogger to mist.

I gave up on all of that, I had too many trays stall from overmisting or under misting. And found that a flipped lid works just fine.


A year ago I had success with fruiting Pan Cyans, but since then I changed the apartment and I had to change my setup and now I'm struggling to set it right. After I mix spawn with substrate should I immediately put it into fruiting chamber (I have small JCM type of setup) or wait to full colonization and pin formation (with the lid flipped) and then put it into fruiting chamber?

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Re: Official Copelandia/Panaeolus Thread [Re: oktopus] * 1
    #29379475 -

For cyans, I let them colonize the substrate completely, case them, and then give them about a day to start colonizing that.

Then into the heated chamber.

The idea is full colonization of the substrate, plus the heat shift should initiate knotting and first flush pins.  And the flipped lid allows some GE while keeping the humidity high.

I only do this for the first flush though, once the knots are set they will keep flushing fine without the lid.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29379487 -

Thank you so much for the clarification! That is really helpful, and I'll do that with my next grow.
I love this community! :heart:

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Re: Official Copelandia/Panaeolus Thread [Re: oktopus]
    #29379887 -

Hi y'all. It's my first time tryna grow pans and have these two jars



The one in the right is from september 28th (from my I had in the freezer) and the one in the left from October 5th

The myc looks a little anemic compared to cubes but not bacterial (unlike my cube jar, which always develop droplets but still fruit in shoeboxes). I'd appreciate your opinion since I think pans have a weirder myc. Thanks in advance


--------------------


Give away list

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Re: Official Copelandia/Panaeolus Thread [Re: mush_monkey] * 3
    #29380362 -




"Polaris Panaeolopsis"
https://mushroomobserver.org/observations/574189


--------------------
In this life, trust is a luxury we cannot afford.

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29380701 -

tree frog said:
Yup.

Standard amount of agar agar.

0.5% full nutrient plates
500ml filtered water
10g agar
2.5g lme

Here's a bvi back cross on the recipe.




Where do you get your glass dishes from?


--------------------
Tent for Tropical Species

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Re: Official Copelandia/Panaeolus Thread [Re: strawboy] * 6
    #29380765 -


TTBVI taking leave of it's rehydrating dry grain host, after being placed on MEA for 48h. (Thanks to Fahtster for sharing this storage method.) Red winter wheat.


--------------------
Peace to you, friend!
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New User Welcome Wagon
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Jump to top Pages: < First | | 1966 | 1967 | 1968 | 1969 | 1970 | 1971 | 1972 | 1973 | 1974 | 1975 | 1976 | 1977 | 1978 | 1979 | 1980 | 1981 | 1982 | 1983 | 1984 | 1985 | 1986 | | Last >

Shop: Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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