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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Wild P. Sub. Germination on Malt Extract Agar Plates (trying to make mycelium bricks)
    #29315803 -

Hi all,

First time poster so if I'm doing it wrong, point fingers and yell!

Currently too busy and don't have a car to go out and hunt anymore (also end of season in the close to city hills? (Went out yesterday and saw very limited number of shrooms)

I'm in South Australia, but we'll keep the identifiables vague.

My workaround is to germinate some spores I got from some P. Subs. I found at a creek (I'm 100% sure they're P. Subs. But realistically I should've gotten a 2nd person's ID), transfer whatever is clean and strong growing on the agar to bulk/spawn whatever the correct term is, essentially just make bricks of P. Sub. Mycelium which I can place outside for next season (and a mate is wanting to see if under his bed is the right climate for fruiting (I doubt it but it doesn't hurt anyone)

Spores were purple/brown.

I inoculated (right word?) 6 initial plates because I assumed the chance would be low of any plate being sterile after introducing wild spores which I printed in the least sanitary way possible (and only printed for about 2 hours) along with using a sterile cotton tip to collect some amount of spores within my dusty dirty room.

After 10 days here are my results. Where should I go from here? One plate looks munted and the others all look pretty clean. Should I get some wood chips or sawdust, boil it, let cool, and add pieces of the germinated agar to it?

Or should I start smaller like with a bag if rice?

Main goal atm is just to make bricks of mycelium so I have a good chance of having an outdoor (mother natures) setup ready for the next season or season after.

But for now, what are peeps thoughts on the plates? Clean? gross? I've got the wrong shrooms? Curiously watching by?

Cheers,

Photos below:













Edited by 009GetRandom (06/08/26 03:26 AM)

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Re: Wild P. Sub. Germination on Malt Extract Agar Plates (trying to make mycelium bricks) [Re: 009GetRandom]
    #29315858 -

Normally you'd use larger plates and spread them out more, so you can see what you've got. (there are 'cell spreaders')  And they should have been started on water agar (7g agar-agar to 500ml H2O) to reduce the potential for mold, bacteria.

You have a very nutritious media there.  I say that because they don't have to go far for food.  Idea is at the second stage (After water agar) is to use low nutrient media (1% MEA or 2% LMEA), to see what their nature is as they spread looking for nutrients.

Each one of those mounds may or may not be mushroom and you'd need to isolate them to find out, assuming your ID of the species is correct.

Edited by anders (08/17/25 09:22 AM)

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Re: Wild P. Sub. Germination on Malt Extract Agar Plates (trying to make mycelium bricks) [Re: anders]
    #29316703 -

Cheers for the tips.

I'll grab some larger plates and begin isolating a couple of the colonies!

The seller listed these plates as light malt extract (so LMEA) and I found it more cost effective to buy more LMEA compared to water agar and MEA separately.(I dont have that sterile of an environment or setup to make doing myself worth while).

I'll send a message asking what the recipe was as (first timer) I never thought that the plates could be too nutrient, hindering isolation

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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