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Mystery #1 and #2 species test grow * 3
    #29267667 -

So I had received two prints on foil, one Mystery #1 and Mystery #2 (as well as an ochra print and one ATL7 print).

The story behind it, is a mislabled strain/species I think? I have to ask them if it's okay if I mention them!
Barely got around to doing it, finally but after this, #2 is gonna come into play also.
They needed some help and I was willing to do so. So now we're trying to figure out what species and variety it could be! :shocked:
So it went Agar>grains>substrate>casing layer.

Rather than a typical CV layer I added way more coarse vermiculite to it,
to sorta get the texture of like a BRF cake or like wet sand (think of building sand castles)
The grain jars (quart) took a bit, about a month to colonize, more or less. Upon S2B in a shoebox,
the spawn DID have the same aroma as stone producers I've worked on before. Slightly different though, maybe just my imagination.
Been in the unmodified tub for four days now, waiting on pins!

Cased!

Will update when something happens, also have a few more jars about three, still waiting for spawning but I'm out of shoe boxes o_o and substrate (sorta)
Fingers crossed! :tongue:


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Nature Reclaims All.

Edited by Wedgie (08/19/25 01:16 AM)

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Re: Mystery #1 species test grow [Re: Wedgie] * 1
    #29267709 -

Very unique lid you have, I had never thought of using foil.
quite clever. :irishtoast:


--------------------
The Hitchhikers Guide
Milkboy's French Press Tek
AIO Rescue Thread
Everything about Agar and it's uses
Guide to Surface Conditions


       
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ
        FRENCHPRESS GANG

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Re: Mystery #1 species test grow [Re: meta_mmxxii] * 1
    #29267712 -

Were they unlabelled prints or marked mystery #1/2?

Any idea if they are cubes?


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Mystery #1 species test grow [Re: KlarnEvil9] * 1
    #29267744 -

meta_mmxxii said:
Very unique lid you have, I had never thought of using foil.
quite clever. :irishtoast:



LOL ohh the foil is there with five toothpick holes for GE
(dunno if that info's outdated) and thank you, an old schoolish way to let them colonize
in a clean dark closet and still get gas exchange while colonzing AND keeping the newly installed substrate or casing moist for
the mycelia.

KlarnEvil9 said:
Were they unlabelled prints or marked mystery #1/2?

Any idea if they are cubes?


Greetings! There were 2, Mystery #1 was a faint and light print, sorta for one use swabbing on agar with a makeshift innoc.
loop (paper clip lol) then the foil was squeaky clean!

Mystery #2 was I believe a darker print, able to be used to inoculate a few agar plates! :laugh: I will go to this one after!

Also sorry went off topic lol, also yes they suspected they were "probably just tamps" and they DO smell like ATL7, but they smell slightly different.

Here are the jars, already making pre truffles just taken seriously a few minutes ago! I'm almost outta substrate though o_o and no shoebox,
might have to make a mini monotub again with Mystery #1. It says I inoculated it June 5th of this year. These are M#1 (Mystery#1)
but needing a new home! These need to be made into a shoebox pie :grin:


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Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie] * 1
    #29267760 -

Most folk go straight into fruiting conditions when preparing their tub.
The mycelium benefits light in all stages of growth, there is no reason to put them in a dark closet.
Your jars do not look very good to me, if this is Cube mycelium, your jars are quite contaminated.
Why is there foil underneath your lids? How did you inoculate your jars.
Seeing a SHIP on your lid, leads me to believe you injected them with a syringe.
If that's the case, did you check them on agar before you injected the grains?


--------------------
The Hitchhikers Guide
Milkboy's French Press Tek
AIO Rescue Thread
Everything about Agar and it's uses
Guide to Surface Conditions


       
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ
        FRENCHPRESS GANG

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Re: Mystery #1 species test grow [Re: meta_mmxxii]
    #29267764 -

This is just how I do it and how it works for me :laugh:
True sorta, it was a inoculated with a syringe. An agar slurry :crazy:

Will keep you guys updated! :cheers:


--------------------
Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: meta_mmxxii]
    #29267809 -

How about actual : CLINGWRAP ?
Clingwrap with some holes poked into.
:ducklol:


--------------------
Psilocybe Semilanceata Experiment

Saving GT contaminated spawn

Golden Teacher 3rd grow

Golden Teacher 2nd grow

πŸ…ƒπŸ„΄πŸ„°πŸ„Ό  πŸ„²πŸ„»πŸ„ΈπŸ„½πŸ„ΆπŸ…†πŸ…πŸ„°πŸ„Ώ

The Hitchhiker's Guide To The Shroomery

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Re: Mystery #1 species test grow [Re: AlexandrDughin]
    #29267814 -

Well that would work for a tub that is dry and the contents/substrate tends to dry out quickly, then clingwrap would apply there and closely to the substrate too, as in right against it.
Besides have you ever tried to ever that for anything at all, not easy getting a nice plastic wrap with out it getting tiny and crumpled up :lol:
All very good info too both of you! :laugh:


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Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie]
    #29271208 -

Update, mystery #1 is pinning good now! She truly is a mystery, she
could even be cubes possibly, I don't know!!
:awejeez:

July5th


July 7th, today!

Of course they're a mystery but possibly tampanensis so this is fun!


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Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie]
    #29272480 -

Soooo anyway these fruits are pretty sweet!
Glad I could help the user figure out what these are but ahah I have no idea.
Like not sure so, when the first harvest comes I'll figure it out.

"FM" mushroom isolate square small container, Mystery #1 in shoebox, long container.

So much stuff to do, I think it's time to mist them again. I figure when the
first wave or "flush" of Mystery #1 I'll harvest. The harvest specimens are going to be photographed, gills, fruits, caps, etc.
Possibly spore prints or spore swabs, though swabs are meh. Mid. I don't bother with them, who does?
No offense. It takes soaking the cotton tip and stuff.



Also started up another SB/shoebox of M1 yo, Mystery #1. This truly
does just seem like a tampanensis o_o but I have my doubts. Don't ask why, but I do because there's
slight similarities yet slight differences that are too difficult to tell without DNA, sequencing yadda yadda RNA? that spinny machine.

Same substrate, spawn, everything. Mystery #1 again!!!
Still colonizing


:cheers:


--------------------
Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie] * 1
    #29272508 -

Tamps or mexi.  They're very closely related (like cubes and ochra) and can be hard to tell apart without microscopy/sequencing.

But I would guess tamps


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Mystery #1 species test grow [Re: tree frog]
    #29272577 -

tree frog said:
Tamps or mexi.  They're very closely related (like cubes and ochra) and can be hard to tell apart without microscopy/sequencing.

But I would guess tamps


Oh my :facepalm: lol oh so true. Ohhh that makes sense,
very true, biology is amazing.

I was kinda thinking the same thing, good on ya! ^^


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Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie] * 1
    #29273476 -

Looking great so far! Tubs and fruits look super happy. The smell told me they were def tamps or mex, also the slender fruit shape and color. Was hoping with pics of fruits that there would be ways to differentiate but based on what TreeFrog is saying, that may not be possible. Thanks for being up to take the journey!

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Re: Mystery #1 species test grow [Re: Actionsaurusrex]
    #29273479 -

Actionsaurusrex said:
Looking great so far! Tubs and fruits look super happy. The smell told me they were def tamps or mex, also the slender fruit shape and color. Was hoping with pics of fruits that there would be ways to differentiate but based on what TreeFrog is saying, that may not be possible. Thanks for being up to take the journey!



Thank you bud :laugh: you're pretty cool!

Hmm sure I agree, but I can also try to take more shots and gills, etc, when I harvest. It looks like it's ready for harvest
though I'm never sure :confused: but it's always a sorta guess when I do with these x_x
OH lol do you think they're ready yet? :laugh:

EDIT: Forgot to mention, yes sir Actionsaur, dude the smell is such a, like,
a very distinct and very familiar, I love it! Almost like a smell that gives me a tiny head rush!

From yesterday Mystery #1 again!

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Re: Mystery #1 species test grow [Re: Wedgie]
    #29275398 -

So an update 😎 went to see my original prints that were sent, something
seemed a bit off though?... maybe, not sure what happened, they looked
different last I checked in the folded paper, in the drawer I left them in.


Oh good point I forgot to mention, DNA testing may be in order I'm thinking.
Here's Mystery #1 again 🀩a new shoebox pin for M#1 again,
I still have one more quart jar of spawn from Mystery#1 but I'm out of
the usual substrate I receive 😿

So I made another shoebox a short while back, πŸ˜‹here she is yesterday
so

Now for the original shoebox with Mystery#1 in it, fruits harvested
(ohhh man hard work without any help) I was also trying to get a few shots
and close up shots of M#1.

A lot of pretty stripes and very photogenic, glad to hear others appreciate
them stripes 😊 speaking of their appearance these mysteries that are #1 seem to have flat caps to sorta "buttercup" shaped caps, indentation downward?


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Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie]
    #29278875 -

:facepalm: I double posted again but both threads are still on topic :jester: 

Pics from today, July 12, MYSTERY #1 🦊


Mush love @Actionsaurusrex 🦊😎 I owe you a drink or a smoke XD

Will update the flush as it mature and the new shoebox!!! Thanks everyone!


...still two ice creams! 😹So awesome thanks so much
Also went ahead with this, the casing mixture is different now.

CVSG (Coir, Vermiculite, Sand and Gypsym) so this might be interesting!
Still this is a new Mystery #1 shoebox so we can still have her around 🐺


Let's add this new casing mix!


Ah well that fun is done, now pinning has been initiated! 🦊


SOOOO while we wait for Mystery #1 OP second flush/wave, and
for the new shoebox, I want to say I'll be attempting
Mystery #2 on agar and then I can go from there!
I'll start some plates soon 🐺 in about a day or two.


--------------------
Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie]
    #29280395 -

I'm thinking this is tampanensis or chincon nindo? 😸πŸ₯³
Although growing closely together they bred possibly genetics? 😹

Isolate "FM" labled ATL#7 (mushroom genetics, mostly yes) but grown close to ATL#7
the isolate N2 I was working on haha on agar.

Ohhh that would explain why Numtoo looked so different after!!!

So this went to> then to >

So grown side by side with "FM" ATL#7 shown here:

I think close breeding, they then created this (numtoo, N2 isolate)


I was guessing this lately but wasn't sure if it sounded too far fetched
to be true πŸ₯³
Still not entirely sure with these genetics or mushroom specimens hah


--------------------
Nature Reclaims All.

Edited by Wedgie (07/13/25 07:20 PM)

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Re: Mystery #1 species test grow [Re: Wedgie] * 1
    #29284460 -

Not entirely sure with the whole genetic breeding thing with the species...


No worries...
Okay after a bit of a break! An update, I still have not started Mystery #2
yet due to epic mysterious reasons for the short break!

None of the caps seem to able to get a print from with
(and I am admittedly awful with swabs and using them) but could still
keep an isolate sample for a while for fun :smile: if that's fine and everything.
Still waiting for the second shoebox to fruit, nothing yet, way more
washed sand in the casing, hahah and gypsym (CaSO 4), that's the only difference. Substrate, everything else is the same.
Plus it doesn't help that it slightly dried out after I forgot about it :facepalm: she's recovered nicely :laugh:

the smell of stone producers, like...
I've mentioned with a few cool cats here, the scent of the mycelium gets me like "whoa!?"

So instead again the OG M#1 shoebox, on flushy two!

Mystery #1 again! Oh yeah definitely a Tampanensis
:awejeez:



and this morning!

Vote yes on more love and support for twisty striped stipes!


--------------------
Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie] * 1
    #29286146 -

Left these a bit longer for today, I couldn't help it, someone else
mentioned how them and/or their family member love the sight of them growing!
So true, who needs a vase and flowers or bowl of fruit with these center pieces?
:awelol:

But this is why also, Mystery #1 reminds me of Ps. Cyanescens haha 😹 
it truly looks amazing! Haven't started Mystery #2 yet forgive me I
keep putting it off x_x
Ps. Cyampanensis 😹
As far as the other shoebox, I think I let her dry out a bit so
she is very focused on the sclerotia πŸ™€ but there ARE pins and truffles
prairie dogging from the surface. Also thinking about to save these fancy little genetics for a bit, so probably simple agar if so.

This worked pretty good for me! The species lives in humid sandy wetlands.

Harvesting from the heavy sand, "feels different" (sand, coir, vermiculite, and gypysum...)
The sand is for gardening but was pressure cooked of course.
Cleaning the stem-butts was a bit messy though :lol:

What treefrog mentioned the Mexicana family, soooo obvious but yeah I
overlooked it. Pretty funny for me though, my brain was just thinking tampanensis.


--------------------
Nature Reclaims All.

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Re: Mystery #1 species test grow [Re: Wedgie] * 1
    #29286178 -

Nice one, cool thread.
:vaped:

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