callmehippie said:

ThurmanMurman said:

Smellyhobbit said:

noomer said:

one more question I guess, how do I know the cake is done done? Just from no more growth? Does the mycelium just die?

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When it no longer suits your needs production wise or it contams out

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I always feel bad treated a sub like it's no longer useful. I wish there was a way for me to let the culture know it did a damn good job and me throwing it in the garbage isn't a reflection of my opinion of it. Sad shit, man.

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Well there  is a re use your spent subs endlessly tek I believe. 

That could be somthin to consider.

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misterswisher said:

Too much moisture or too little can cause that.

Second flush will be a lot better if it was a too much moisture issue. Looks like they’re aborting. That’s why they’re dark

Almost also looks like you got some bacterial blotching, idk if anyone here uses that term but gormet growers do. Water touching the tissue causes it. Avoid letting the fruits get water on them.

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Jrey16 said:

misterswisher said:

Too much moisture or too little can cause that.

Second flush will be a lot better if it was a too much moisture issue. Looks like they’re aborting. That’s why they’re dark

Almost also looks like you got some bacterial blotching, idk if anyone here uses that term but gormet growers do. Water touching the tissue causes it. Avoid letting the fruits get water on them.

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Thanks. Any potency lost you think?

And yes I used bacterial grain spawn that metabolites in them.

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chris77 said:

Jrey16 said:

misterswisher said:

Too much moisture or too little can cause that.

Second flush will be a lot better if it was a too much moisture issue. Looks like they’re aborting. That’s why they’re dark

Almost also looks like you got some bacterial blotching, idk if anyone here uses that term but gormet growers do. Water touching the tissue causes it. Avoid letting the fruits get water on them.

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Thanks. Any potency lost you think?

And yes I used bacterial grain spawn that had metabolites in them.

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misterswisher said:

They’ll do the job still trust. IMO smaller fruits hit harder but other disagree.

Just make tea out of the slimy ones if you keep them lol. Don’t recommend eating them raw.

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noomer said:

Can I put spores from a print directly into agar? Sorry if that’s a no brainer question.

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Yes. I usually hold print at an angle so not really over the plate and scrape print with scalpel to drop a little cloud of spores over the plate.

Flame sterilize the scalpel first, then quench near outer edge of receiving clean plate so you're not cooking your spores.

Some use more precise methods but this makes a wide variety of germination plate choices to transfer from.

Here's a plate I did this with, then the same plate after growth. Left side of plate I actually pressed print into the plate since I'd used print a few times and was about to toss it. I do that holding plate sideways. Never want to have hand over plate. Hand is gloved and wiped with 70 percent ISO alcohol.

hyperspace_viking said:

spawned this tub 1 week ago, 1:4 wheat spawn to coir (95% coir and 5% verm). Looks like the fastest and most successful monotub i have done. So i dont want to fuck up now. Is it ready for mist and cracking the lid or should i give it a couple of days more? Water is starting to pool in the corners from condensation and i am worried that the excess water will fuck things up if it doesnt evaporate soon.

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if water is pooling don't mist. if water is pooling you could def crack the lid. looks great though. mostly litfa. imo. looks like it's gonna pop!

ThurmanMurman said:

Smellyhobbit said:

noomer said:

one more question I guess, how do I know the cake is done done? Just from no more growth? Does the mycelium just die?

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When it no longer suits your needs production wise or it contams out

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I always feel bad treating a sub like it's no longer useful. I wish there was a way for me to let the culture know it did a damn good job and me throwing it in the garbage isn't a reflection of my opinion of it. Sad shit, man.

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Phaethon said:

nocap said:

BRF teks seems to be for niche usage - agar to grain is general purpose, especially once you have a good culture.

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Just so it's not lost here, one of the things the linked tek is great for is getting a good culture from a bacterial one, or from spores that don't want to germinate on agar. It's a pretty easy way to get clones, too, with a little more idea of how they will express than plate pins, since they grow on a more typical fruiting medium and with more space to grow.

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