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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope] * 2
    #29143484 -

Even 100% clean spawn can stall if it's too clumpy or too wet.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29143486 -


This is still my first grow, this is my biggest shroom so far, but it looks kinda funky, is the head gonna fold out and become a regular looking shroom? Or is something wrong with it? Its cubensis, strain should be mckennaii.

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 2
    #29143545 -

Thanks all.

tree frog said:
Even 100% clean spawn can stall if it's too clumpy or too wet.


What I then wondered is when mycelium is colonizing grains, it can not revert to colonizing liquid? So, did I just now understand there are liquid and non-liquid vegetative grow states? OK, I looked it up. Thanks to GPT (awesome! If I say so myself):

//
Q:
Mycelium can grow in liquid culture, but when growing on grain, it seems to not go back to growing in liquid like water. How come?

CGPT:
Mycelium behaves differently in liquid culture compared to solid substrates like grain due to how it adapts to its environment. Here are some reasons why mycelium struggles to revert to liquid growth after colonizing grain:

Structural Adaptation – In liquid culture, mycelium grows in a free-floating, filamentous form, often as strands or clumps. On solid substrates like grain, the mycelium becomes more structured, forming dense networks that anchor to the grains. Once it has adapted to this denser, more interconnected form, it doesn’t easily transition back to the free-floating state required for liquid culture.

Protective Coating & Hydrophobicity – Mycelium grown on grain can develop hydrophobic properties, meaning it resists water absorption. This is particularly true for some species that produce extracellular substances or hydrophobic mycelial surfaces that make it harder to disperse in liquid.

Lack of Agitation & Oxygen Availability – In liquid culture, mycelium is typically stirred or shaken to keep it dispersed and oxygenated. When transferring from grain back to liquid, the mycelium might clump together or sink, limiting oxygen exposure and slowing growth.

Nutrient Availability – Mycelium on grain has adapted to breaking down starches and complex nutrients. If transferred to plain water or a simple liquid medium, it may lack the necessary enzymes or may not recognize the new environment as a suitable growth medium.

Developmental Stage – Mycelium on grain is often closer to the fruiting stage, meaning its metabolic pathways may have shifted away from the type of growth needed for expansion in liquid culture.

If you want to get mycelium from grain back into a liquid culture, you can try:

Blending or chopping it finely before adding it to the liquid.
Adding nutrients similar to those found in grain.
Using gentle agitation to encourage dispersion.
//



Maybe, if you have water in your jar, make sure the LC hits the water. Maybe also make sure it has some nutes in it. Hm. Might it speed up thing? Is it a really bad idea to give my next batch some sugar water added and see whether this 'LC X grains' jars work? Or am I'm just being stupid here? Probably the latter, but eager to try. Not sure how many days advantage this will give, also relative to higher contam risk. Eh, I forgot the question.

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork] * 2
    #29143562 -

u would have to have alot of water in the jar to even be worried :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Re: Ask Quick Questions, Get Quick Answers [Re: tripdawg420]
    #29143573 -

Man. This thread moves quick!  Also, unfortunately my unintentional post (that also belonged here) is now gone too.  So I'm gonna ask again(I guess that is what i get for saying "wrong thread" 
Anyways, it's been a while since I have grown, and Never PE.  How long until these guys are probably ready to harvest the first flush.  Only my 3rd grow, other 2 were PF based and I hear the dickbois are a whole different animal.


(My appologizes ahead of time if my 3 times skimming for my posts got glossed by my absent-minded arse.

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Re: Ask Quick Questions, Get Quick Answers [Re: sk8cracka] * 1
    #29143579 -

With PE you want them to soften up like Marshmallows. They don’t throw many spores and when they do it’s only a few fruits. Typically the largest of the bunch.

I’ve had them go up to two weeks after pinning but I’d say my average is 7-10 days from pin and 5-7 from the veil breaking. This timeline is also dependent on the starting culture. Some are just faster than others.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Ask Quick Questions, Get Quick Answers [Re: OLDDAD77]
    #29143641 -

Mwj12977 said:
With PE you want them to soften up like Marshmallows. They don’t throw many spores and when they do it’s only a few fruits. Typically the largest of the bunch.

I’ve had them go up to two weeks after pinning but I’d say my average is 7-10 days from pin and 5-7 from the veil breaking. This timeline is also dependent on the starting culture. Some are just faster than others.


:whathesaid:
I've had PE tubs take 2 months to consolidate, and then another 2 weeks from pin to being ready to harvest.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29143656 -

I got my hands or a really fast PE7 culture last year. It was one of the fastest fruiters I’ve ever grown, didn’t blob either.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #29143699 -


mushboy said:
nah just dump some water in the box let it sit and pour out what doesnt get soaked up. like 2/3 cup should be good.


Sweet thanks. Do you let it sit for 12+ hours or???

Also what do you guys do with side pins or fruits growing on the bottom of the sub in a shoebox?  Is it safe to remove the substrate to harvest the fruits?  Or just leave them alone and count it as a loss?

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Re: Ask Quick Questions, Get Quick Answers [Re: suicideprevention]
    #29143741 -

12 hrs is fine.


Fruits on bottom...If sub is easily moved during harvest I will pick them .

If not they should be fine to leave

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Re: Ask Quick Questions, Get Quick Answers [Re: mush_monkey]
    #29143917 -

I'm using this type of jars (Nescafe repurposed ground coffee ones) to store my dried shrooms.




I'm pretty sure that the lid is made from PP5.  If I modified it with a hole for GE, do you think it would be good as a spawn jar? I mean in terms of the lid preventing bacteria from getting in.


--------------------


Give away list

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Re: Ask Quick Questions, Get Quick Answers [Re: mush_monkey] * 1
    #29143921 -

You could always wrap the edge with cling wrap or parafilm to seal between lid and container.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29143925 -

Thanks! That's what I just started doing with my screw-top jars. I was wondering it this jars would be safer


--------------------


Give away list

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Re: Ask Quick Questions, Get Quick Answers [Re: mush_monkey] * 2
    #29143926 -

I only use canning jars for grain spawn. I also wrap the lid/jar combo once they're inoculated.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29144051 -

Spikey mycelium in a jar is a sign of bacteria, correct?








This is my first round of jars since moving away from oats. While it is an improvement over how the oat jars look, if it's still bacterial it means that my source is from my culture or sterile procedures.

Oat jar for reference:



I've already tried cabin sequestering and josex poke, I'm thinking of trying 3% water agar next. Any suggestions are welcome.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 1
    #29144081 -

Do you still have the spores?

I've been liking starting spores on brf pucks, makes it easy to take a tiny transfer and it naturally sequesters bacteria.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29144091 -

tree frog said:
Do you still have the spores?

I've been liking starting spores on brf pucks, makes it easy to take a tiny transfer and it naturally sequesters bacteria.


Not of this strain, but that's actually how I started out the inoculation.

I'm only assuming that the bacteria is from the culture. I don't get any biofilm, but I'm assuming that I'm dealing with the kind that grows in conjunction with the mycelium.

I believe my SAB work is good as well, because I haven't seen any biofilm at all on a transfer plate in about 5 months. I used to get it all the time.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (03/02/25 07:49 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29144209 -

Can somebody link me to some good sfds to buy. Like the big ones that don't have adhesive.


Or do these look good and a have  good price?



--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: mush_monkey] * 1
    #29144259 -

mush_monkey said:
I'm using this type of jars (Nescafe repurposed ground coffee ones) to store my dried shrooms.




Invest in a vacuum sealer

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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet]
    #29144283 -

Pc'd two jars with about the same amount. . .why did ((both) end up so damn full, but one to the brim  and all funky gunky inside?!

2 is the ugly one 1 is normal.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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