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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: 2020twicebaked]
    #29132986 -

2020twicebaked said:
Need some help, new to agar. These two are from agar to agar, bumpy edges not symmetrical not sure if it's contam or not.







Some of that looks like mold to me


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 2
    #29132987 -

Jrey16 said:
I made some transfers onto LME agar that was overcooked and caramelized and a few of them look like this. The mycelium is very puffy and it grew upwards and it's touching the roof of the plate. Are these plates still okay?





And all of these plates in the pic below are also transfers that I put onto caramelized and overcooked agar. It looks clear in the picture but there's actually a bunch of caramelized LME on the bottom of the plates.
I am used to uniform rhizomorphic growth. Do these plates look okay? Do they look like this because I used overcooked agar? or maybe because these are new strains I am trying out for the first time that I'm not used to? Or am I just OCD with this shit and want symmetrical rhizomorphic growth? They are all cubensis




Erythrosine..


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29132988 -

Had to look up what that meant lol. Yes I used McCormicks red food coloring. A little too much ended up spilling out when I put it into the agar bottle actually. Is that a problem?

I always used the same red dye and never had any problems.


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29132993 -

I dunno, probably just an old Rum tale.
But food dyes are chems and unnecessary for the process foe the little help they MAY give. That myce looks good and strong. Just looks like it hates the agar. Let it grow out more, make your next dishes without coloring, pour them thin, and transfer again is my advice.


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133000 -

Ahh damn I just made 40 plates of blue colored agar smh. Yea I'll skip the dye next time.

Do you mean I should make transfers from these plates because all of them might not be clean enough to put to grain once fully colonized?


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133003 -

These are the last plates I made with red dye but I barely put any dye in. Big difference





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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 2
    #29133007 -

Damn i would've taken those to grain from there. Don't worry about the dye use what ya got. But I ment transfer to another plate again cau the growths dont look very uniform. But if they came from those plates it makes me wonder about the agar composition even more. See how they grow out, if they get more uniform then id test to grain as well as transferring to new plates. Spaghetti against the wall ftw


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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29133009 -

Ahh ook.now it makes sense.  Thanks.


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133019 -

Wasn’t there discussion about the red dye being the one that could affect growth the most?

Second question.
Should I have used effect in that sentence?


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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 1
    #29133021 -

I used to talk about it a lot but it's so unconfirmed. Shit was just made illegal to use in the US as well as other countries. It's bad, all colorings are bad imo even if the work. Colouration isnt the same as verification or some smart thing Stipe would say. Keep agar simple, baseline, without variables is the best way to achieve your own version of consistency of growth.


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Edited by rumfor69 (02/21/25 01:19 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133023 -

Can you confirm for me that red 40 is illegal now?
:deathrush:


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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29133024 -

GenesisCorrupted said:
Wasn’t there discussion about the red dye being the one that could affect growth the most?

Second question.
Should I have used effect in that sentence?



Effect and affect are both correct but one can mean past tense and present tense better...not sure which one. The mushroom effects affected me..


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133026 -

I thought it was red 5


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133027 -

Yeah, that’s what I was thinking. The red dye is going to affect what you’re working on. Which will have a negative effect on your results.
rumfor69 said:
I thought it was red 5


That one’s shitty too. Red 40s still toxic though.
That’s your code red an MnMs, tons of stuff actually.


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Edited by GenesisCorrupted (02/21/25 01:00 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 2
    #29133030 -

I'll boof em all. Push mnms up there like a reverse pez dispenser


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133031 -

rumfor69 said:
Damn i would've taken those to grain from there. Don't worry about the dye use what ya got. But I ment transfer to another plate again cau the growths dont look very uniform. But if they came from those plates it makes me wonder about the agar composition even more. See how they grow out, if they get more uniform then id test to grain as well as transferring to new plates. Spaghetti against the wall ftw


Oh I did take these to grain and had nice grows. I was just pointing out the big difference between less red dye vs. too much dye because this time I put way too much dye and they're growing all weird now with too much dye.
Idk if it's really the food coloring or other factors like my new Presto PC I'm not used to that actually gets up to 15 PSI lol. But i'm betting it's the dye. I put a shit ton on accident. I guess i'll be making more plates again and definitely not putting any dye in it.


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133032 -

Did you know that you can make flavored vodka out of each individual color of skittle?


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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29133033 -

Like putting a jolly rancher into a Zema?


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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #29133034 -

I don’t see why not.
Takes a while for them to dissolve, then you just strain out the bits with a coffee filter.


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29133035 -

Ahhhhhhh.....omg.   

What could've happened here?

This is a pf jar straight out the PC. U  Sat  with foil on grow room shelf for 2days un- inoculated. I go to inoculate it and see a bunch of white. Jar goes in quarantine. This how it looks a few days later.



..


Thats one outta 12 jars.

3 more that were inoculated are showing black pin nold. But that's not unusual or confusing. 





Im just super  confused about the one that hadn't been inoculated. Where did the contam come from?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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